Homing of stem cells to sites of inflammatory brain injury after intracerebral and intravenous administration: a longitudinal imaging study

Introduction This study aimed to determine the homing potential and fate of epidermal neural crest stem cells (eNCSCs) derived from hair follicles, and bone marrow-derived stem cells (BMSCs) of mesenchymal origin, in a lipopolysaccharide (LPS)-induced inflammatory lesion model in the rat brain. Both eNCSCs and BMSCs are easily accessible from adult tissues by using minimally invasive procedures and can differentiate into a variety of neuroglial lineages. Thus, these cells have the potential to be used in autologous cell-replacement therapies, minimizing immune rejection, and engineered to secrete a variety of molecules. Methods Both eNCSCs and BMSCs were prelabeled with iron-oxide nanoparticles (IO-TAT-FITC) and implanted either onto the corpus callosum in healthy or LPS-lesioned animals or intravenously into lesioned animals. Both cell types were tracked longitudinally in vivo by using magnetic resonance imaging (MRI) for up to 30 days and confirmed by postmortem immunohistochemistry. Results Transplanted cells in nonlesioned animals remained localized along the corpus callosum. Cells implanted distally from an LPS lesion (either intracerebrally or intravenously) migrated only toward the lesion, as seen by the localized MRI signal void. Fluorescence microscopy of the FITC tag on the nanoparticles confirmed the in vivo MRI data, Conclusions This study demonstrated that both cell types can be tracked in vivo by using noninvasive MRI and have pathotropic properties toward an inflammatory lesion in the brain. As these cells differentiate into the glial phenotype and are derived from adult tissues, they offer a viable alternative autologous stem cell source and gene-targeting potential for neurodegenerative and demyelinating pathologies. </br

Dynamics of Tunneling Centers in Metallic Systems

Dynamics of tunneling centers (TC) in metallic systems is studied, using the technique of bosonization. The interaction of the TC with the conduction electrons of the metal involves two processes, namely, the screening of the TC by electrons, and the so-called electron assisted tunneling. The presence of the latter process leads to a different form of the renormalized tunneling frequency of the TC, and the tunneling motion is damped with a temperature dependent relaxation rate. As the temperature is lowered, the relaxation rate per temperature shows a steep rise as opposed to that in the absence of electron assisted process. It is expected that this behavior should be observed at very low temperatures in a careful experiment. The present work thus tries to go beyond the existing work on the {\it dynamics} of a two-level system in metals, by treating the electron assisted process.Comment: REVTeX twocolumn format, 5 pages, two PostScript figures available on request. Preprint # : imsc 94/3

Intratympanic methylprednisolone versus gentamicin in patients with unilateral Ménière's disease: a randomised, double-blind, comparative effectiveness trial

Background Ménière’s disease is characterised by severe vertigo attacks and hearing loss. Intratympanic gentamicin,the standard treatment for refractory Ménière’s disease, reduces vertigo, but damages vestibular function and can worsen hearing. We aimed to assess whether intratympanic administration of the corticosteroid methylprednisolone reduces vertigo compared with gentamicin. Methods In this double-blind comparative eff ectiveness trial, patients aged 18–70 years with refractory unilateral Ménière’s disease were enrolled at Charing Cross Hospital (London, UK) and Leicester Royal Infirmary (Leicester, UK). Patients were randomly assigned (1:1) by a block design to two intratympanic methylprednisolone(62·5 mg/mL) or gentamicin (40 mg/mL) injections given 2 weeks apart, and were followed up for 2 years. All investigators and patients were masked to treatment allocation. The primary outcome was vertigo frequency over the final 6 months (18–24 months after injection) compared with the 6 months before the first injection. Analyses were done in the intention-to-treat population, and then per protocol. This trial is registered with ClinicalTrials.gov, number NCT00802529. Findings Between June 19, 2009, and April 15, 2013, 256 patients with Ménière’s disease were screened, 60 of whom were enrolled and randomly assigned: 30 to gentamicin and 30 to methylprednisolone. In the intention-to-treat analysis (ie, all 60 patients), the mean number of vertigo attacks in the fi nal 6 months compared with the 6 months before the fi rst injection (primary outcome) decreased from 19·9 (SD 16·7) to 2·5 (5·8) in the gentamicin group (87% reduction) and from 16·4 (12·5) to 1·6 (3·4) in the methylprednisolone group (90% reduction; mean diff erence –0·9,95% CI –3·4 to 1·6). Patients whose vertigo did not improve after injection (ie, non-responders) after being assessed by an unmasked clinician were eligible for additional injections given by a masked clinician (eight patients in the gentamicin group vs 15 in the methylprednisolone group). Two non-responders switched from methylprednisolone to gentamicin. Both drugs were well tolerated with no safety concerns. Six patients reported one adverse event each: three in the gentamicin group and three in the methylprednisolone group. The most common adverse event was minor ear infections, which was experienced by one patient in the gentamicin group and two in the methylprednisolone group. Interpretation Methylprednisolone injections are a non-ablative, effective treatment for refractory Ménière’s disease. The choice between methylprednisolone and gentamicin should be made based on clinical knowledge and patient circumstances

Evidence of Electromagnetic Absorption by Collective Modes in the Heavy Fermion Superconductor UBe13

We present results of a microwave surface impedance study of the heavy fermion superconductor UBe13. We clearly observe an absorption peak whose frequency- and temperature-dependence scales with the BCS gap function. Resonant absorption into a collective mode, with energy approximately proportional to the superconducting gap, is proposed as a possible explantation

Tax avoidance as an anti-austerity issue: the progress of a protest issue through the public sphere

Theorists of left and right agree that periods of crisis are fertile times at which to precipitate change. However, protesters on the periphery of the public sphere must overcome barriers, or what Habermas called ‘sluice gates’, if their discourse is to be publicly and politically influential. This study of newspaper discourse and activity in parliament and the public sphere over a six year period takes tax justice campaigning in the UK as a case study, and in particular protest group UK Uncut’s attempt to mobilize opposition to austerity by advocating a crackdown on tax avoidance as an alternative to cuts. It finds that whilst UK Uncut successfully amplified existing arguments previously raised by experts, trade unions and the left-leaning press, austerity barely figured in debate about tax avoidance once it was picked up by other actors in the public sphere on the other side of the 'sluice gates'. The reasons for this were structural and discursive, related to the role and interests of receptive actors at the institutional centre of the public sphere, and their ability, along with the conservative press, to transform the moral framing of tax avoidance from the injustice of making the poor pay for the financial crisis through cuts, into the 'unfairness' of middle class earners paying higher taxes than wealthier individuals and corporations. The latter reifies the 'hardworking taxpayer', and implies a more instrumental and clientalistic relationship to the state, and an essentially neoliberal sense of fairness. Where neoliberal ideology was challenged, it was in social conservative terms – nationalist opposition to globalisation, framing multinational corporations as a threat to the domestic high street – rather than protesters’ social democratic challenge to market power and social injustice. This indicates how a progressive message from the periphery can be co-opted into the currently resurgent right-wing populism

The Intrinsic Quantum Excitations of Low Temperature Glasses

Several puzzling regularities concerning the low temperature excitations of glasses are quantitatively explained by quantizing domain wall motions of the random first order glass transition theory. The density of excitations agrees with experiment and scales with the size of a dynamically coherent region at $T_g$, being about 200 molecules. The phonon coupling depends on the Lindemann ratio for vitrification yielding the observed universal relation $l/\lambda \simeq 150$ between phonon wavelength $\lambda$ and mean free path $l$. Multilevel behavior is predicted to occur in the temperature range of the thermal conductivity plateau.Comment: 4 pages, submitted to PR

Two-Dimensional Polymers with Random Short-Range Interactions

We use complete enumeration and Monte Carlo techniques to study two-dimensional self-avoiding polymer chains with quenched ``charges'' $\pm 1$. The interaction of charges at neighboring lattice sites is described by $q_i q_j$. We find that a polymer undergoes a collapse transition at a temperature $T_{\theta}$, which decreases with increasing imbalance between charges. At the transition point, the dependence of the radius of gyration of the polymer on the number of monomers is characterized by an exponent $\nu_{\theta} = 0.60 \pm 0.02$, which is slightly larger than the similar exponent for homopolymers. We find no evidence of freezing at low temperatures.Comment: 4 two-column pages, 6 eps figures, RevTex, Submitted to Phys. Rev.

Scaling and universality in the anisotropic Kondo model and the dissipative two-state system

Scaling and universality in the Ohmic two-state system is investigated by exploiting the equivalence of this model to the anisotropic Kondo model. For the Ohmic two-state system, we find universal scaling functions for the specific heat, $C_{\alpha}(T)$, static susceptibility, $\chi_{\alpha}(T)$, and spin relaxation function $S_{\alpha}(\omega)$ depending on the reduced temperature $T/\Delta_{r}$ (frequency $\omega/\Delta_{r}$), with $\Delta_{r}$ the renormalized tunneling frequency, and uniquely specified by the dissipation strength $\alpha$ ($0<\alpha<1$). The scaling functions can be used to extract $\alpha$ and $\Delta_{r}$ in experimental realizations.Comment: 5 pages (LaTeX), 4 EPS figures. Minor changes, typos corrected, journal reference adde