Population structure and variance effective size of red snapper (Lutjanus campechanus) in the northern Gulf of Mexico*

We assayed allelic variation at 19 nuclear-encoded microsatellites among 1622 Gulf red snapper (Lutjanus campechanus) sampled from the 1995 and 1997 cohorts at each of three offshore localities in the northern Gulf of Mexico (Gulf). Localities represented western, central, and eastern subregions within the northern Gulf. Number of alleles per microsatellite per sample ranged from four to 23, and gene diversity ranged from 0.170 to 0.917. Tests of conformity to Hardy-Weinberg equilibrium expectations and of genotypic equilibrium between pairs of micro-satellites were generally nonsignificant following Bonferroni correction. Significant genic or genotypic heterogeneity (or both) among samples was detected at four microsatellites and over all microsatellites. Levels of divergence among samples were low (FST ≤0.001). Pairwise exact tests revealed that six of seven “significant” comparisons involved temporal rather than spatial heterogeneity. Contemporaneous or variance effective size (NeV) was estimated from the temporal variance in allele frequencies by using a maximum-likelihood method. Estimates of NeV ranged between 1098 and >75,000 and differed significantly among localities; the NeV estimate for the sample from the northcentral Gulf was >60 times as large as the estimates for the other two localities. The differences in variance effective size could ref lect differences in number of individuals successfully reproducing, differences in patterns and intensity of immigration, or both, and are consistent with the hypothesis, supported by life-history data, that different “demographic stocks” of red snapper are found in the northern Gulf. Estimates of NeV for red snapper in the northern Gulf were at least three orders of magnitude lower than current estimates of census size (N). The ratio of effective to census size (Ne/N) is far below that expected in an ideal population and may reflect high variance in individual reproductive success, high temporal and spatial variance in productivity among subregions or a combination of the two

Tales of the Olympic city: Memory, narrative and the built environment = Historias de la ciudad olímpica: memoria, narrativa y el entorno construido

The Olympics have a greater, more profound and more pervasive impact on the urban fabric of their host cities than any other sporting or cultural event. This paper is concerned with issues of memory and remembering in Olympic host cities. After a contextual introduction, it employs a case study of the Queen Elizabeth Olympic Park (QEOP), the main event space for the London 2012 Summer Games, to supply insight into how to read the urban traces of Olympic memory. Three key themes are identified when interpreting the memories associated with the Park and its built structures, namely: treatment of the area’s displaced past, memorializing the Games, and with memory legacy. The ensuing discussion section then adopts a historiographic slant, stressing the importance of narrative and offering wider conclusions about Olympic memory and the city. = El impacto de los Juegos Olímpicos en el tejido urbano de las ciudades anfitrionas es mayor, más profundo y más generalizado que el de cualquier otro evento deportivo o cultural. Este trabajo analiza temas relacionados con la memoria y el recuerdo en las ciudades anfitrionas de los Juegos Olímpicos. Tras introducir del contexto, se utiliza un estudio de caso del Parque Olímpico Queen Elizabeth (QEOP, por sus siglas en inglés), el principal espacio de los Juegos de Verano de Londres 2012, para plantear un nuevo enfoque sobre cómo leer las huellas urbanas de la memoria olímpica. Se identifican tres temas clave al interpretar los recuerdos asociados con el Parque y sus estructuras construidas, a saber: el tratamiento del pasado desplazado del área, la conmemoración de los Juegos y el legado de la memoria. La sección de discusión adopta un enfoque historiográfico, subrayando la importancia de la narrativa y ofreciendo gran variedad de conclusiones sobre la memoria olímpica y la ciudad

Historias de la ciudad olímpica: memoria, narrativa y el entorno construido

The Olympics have a greater, more profound and more pervasive impact on the urban fabric of their host cities than any other sporting or cultural event.  This paper is concerned with issues of memory and remembering in Olympic host cities.  After a contextual introduction, it employs a case study of the Queen Elizabeth Olympic Park (QEOP), the main event space for the London 2012 Summer Games, to supply insight into how to read the urban traces of Olympic memory.  Three key themes are identified when interpreting the memories associated with the Park and its built structures, namely: treatment of the area’s displaced past, memorializing the Games, and with memory legacy.  The ensuing discussion section then adopts a historiographic slant, stressing the importance of narrative and offering wider conclusions about Olympic memory and the city.El impacto de los Juegos Olímpicos en el tejido urbano de las ciudades anfitrionas es mayor, más profundo y más generalizado que el de cualquier otro evento deportivo o cultural. Este trabajo analiza temas relacionados con la memoria y el recuerdo en las ciudades anfitrionas de los Juegos Olímpicos. Tras introducir del contexto, se utiliza un estudio de caso del Parque Olímpico Queen Elizabeth (QEOP, por sus siglas en inglés), el principal espacio de los Juegos de Verano de Londres 2012, para plantear un nuevo enfoque sobre cómo leer las huellas urbanas de la memoria olímpica. Se identifican tres temas clave al interpretar los recuerdos asociados con el Parque y sus estructuras construidas, a saber: el tratamiento del pasado desplazado del área, la conmemoración de los Juegos y el legado de la memoria. La sección de discusión adopta un enfoque historiográfico, subrayando la importancia de la narrativa y ofreciendo gran variedad de conclusiones sobre la memoria olímpica y la ciudad

Microsatellite primers for red drum (Sciaenops ocellatus)

In this note, we document polymerase-chain-reaction (PCR) primer pairs for 101 nuclear-encoded microsatellites designed and developed from a genomic library for red drum (Sciaenops ocellatus). Details of the genomic library construction, the sequencing of positive clones, primer design, and PCR protocols may be found in Karlsson et al. (2008). The 101 microsatellites (GENBA NK Accession Numbers EU015882-EU015982) were amplified successfully and used to genotype 24 red drum obtained from Galveston Bay, Texas (Table 1). A total of 69 of the microsatellites had an uninterrupted (perfect) dinucleotide motif, and 30 had an imperfect dinucleotide motif; one microsatellite had an imperfect tetranucleotide motif, and one had an imperfect and compound motif (Table 1 ). Sizes of the cloned alleles ranged from 84 to 252 base pairs. A ‘blast’ search of the GENBANK database indicated that all of the primers and the cloned alleles were unique (i.e., not duplicated)

Population structure, long-term connectivity, and effective size of mutton snapper (Lutjanus analis) in the Caribbean Sea and Florida Keys

Genetic structure and average long-term connectivity and effective size of mutton snapper (Lutjanus analis) sampled from offshore localities in the U.S. Caribbean and the Florida Keys were assessed by using nuclear-encoded microsatellites and a fragment of mitochondrial DNA. No significant differences in allele, genotype (microsatellites), or haplotype (mtDNA) distributions were detected; tests of selective neutrality (mtDNA) were nonsignificant after Bonferroni correction. Heuristic estimates of average long-term rate of migration (proportion of migrant individuals/generation) between geographically adjacent localities varied from 0.0033 to 0.0054, indicating that local subpopulations could respond independently of environmental perturbations. Estimates of average longterm effective population sizes varied from 341 to 1066 and differed significantly among several of the localities. These results indicate that over time larval drift and interregional adult movement may not be sufficient to maintain population sustainability across the region and that there may be different demographic stocks at some of the localities studied. The estimate of long-term effective population size at the locality offshore of St. Croix was below the minimum threshold size considered necessary to maintain the equilibrium between the loss of adaptive genetic variance from genetic drift and its replacement by mutation. Genetic variability in mutton snapper likely is maintained at the intraregional level by aggregate spawning and random mating of local populations. This feature is perhaps ironic in that aggregate spawning also renders mutton snapper especially vulnerable to overexploitation

Mitochondrial DNA Diversity and Population Structure of Spotted Seatrout (Cynoscion nebulosus) in Coastal Waters of the Southeastern United States

Restriction-site variation in mitochondrial (mt)DNA was examined among 470 spotted seatrout (Cynoscion nebulosus) sampled from eight localities in the northern Gulf of Mexico (Gulf) and two localities along the southeastern (Atlantic) coast of the United States. mtDNA fragment patterns generated 81 distinct mtDNA haplotypes (genotypes), three of which were found in 64% of the individuals assayed. Nucleotide sequence divergence among mtDNA haplotypes ranged from 0.148 to 1.808% and averaged (±SD) 0.676 ± 0.296%. Significant heterogeneity in mtDNA haplotype frequencies was detected between regions (i.e., Gulf vs Atlantic) and among sample localities from the northern Gulf. The latter appears to stem primarily (but not exclusively) from heterogeneity among samples from the western Gulf. Frequency plots of two of the common mtDNA haplotypes revealed strong east-west clines across the northern Gulf, with distinctive frequency discontinuities ( steps ) between Gulf and Atlantic samples and between the westernmost sample in the Gulf (the lower Laguna Madre in Texas) and the next-most geographically proximate sample to the east. Spatial autocorrelation analysis of mtDNA haplotype frequencies among samples from the northern Gulf revealed a strong isolation-by-distance effect. These results support the hypothesis that spotted seatrout are spatially subdivided into discrete subpopulations or stocks. Divergence between Gulf and Atlantic subpopulations is likely related to historical vicariance, stemming from climatic changes occurring during glacial times. Other factors likely reduce present-day gene flow between subpopulations in the two regions. Divergence among subpopulations in the northern Gulf is more likely due to behavioral factors that limit (female) dispersal from a natal bay or estuary. The latter is consistent with studies of life history and returns from mark-and-recapture experiments. mtDNA diversity, an index of evolutionary effective (female) population size, also differed significantly among samples, with spotted seatrout from the lower Laguna Madre possessing appreciably reduced mtDNA diversity. This indicates a reduction in the effective number of females in the lower Laguna Madre and suggests that careful monitoring of spotted seatrout in the estuary may be warranted

From A to B: The Summer Olympics, 1896–2008

Providing a full overview of the changing relationship between cities and the Olympic events, this substantially revised and enlarged edition builds on the success of its predecessor. Its coverage takes account of important new scholarship as well as adding reflections on the experience of staging Beijing 2008 and Vancouver 2010, the state of preparations for London 2012, and the plans for the Games scheduled for Sochi in 2014 and Rio de Janeiro 2016. The book is divided into three parts that provide overviews of the urban legacy of the four component Olympic festivals; systematic surveys of five key aspects of activity involved in staging the Olympics; and ten chronologically arranged portraits of host cities. As controversy over the growing size and expense of the Olympics continues, this timely assessment of the Games’ development and the complex agendas that host cities attach to the event will be essential reading for urban and sports historians, urban geographers, planners and all concerned with understanding the relationship between cities and culture

Longevity and reinvention: Venetianization and the Biennale

This chapter explores the development and urban implications of the Biennale. It contains five main parts. After considering the events and circumstances that led to the Biennale’s foundation in 1895, the ensuing section examines the politically inspired festivalization that characterised the 1930s, its growth after 1945 and its increasing ‘Venetianization’ – the term used by Clarissa Ricci (2010, 105) to describe the festival’s tendency to spread spatially from its original hub in the Giardini into locations scattered throughout the rest of the city. The final parts look at current issues, including the problems facing the city’s historic core and the rising disquiet of citizens feeling themselves overwhelmed by the impact of mass cultural tourism on the city’s everyday life. It is noted that the hiatus in activity caused by the response to COVID-19 has fed calls for rethinking Venice’s relationship with art, tourism, and urban development. The desire for a ‘new normal’ that is expressed in some quarters, juxtaposed with the views of those who wish to restore the status quo ante, provides an important dynamic for future discourse and practice