Production of extracellular aspartic protease in submerged fermentation with Mucor mucedo DSM 809

Fungal milk-clotting enzymes have gained value as bovine Chymosin substitutes in the cheese industry. In this work, the effects of culture conditions on the production of extracellular milk clotting enzymes from Mucor mucedo DSM 809 in submerged fermentation were studied. The maximum activity was observed after 48 h of cultivation at 24°C in Erlenmeyer flasks. The optimized initial pH and shaking speed for enzyme production were 4.5 and 220 rpm, respectively. Glucose at a concentration of 1% (w/v) was the best carbon source for the production of enzyme among the carbohydrates examined (glucose, fructose, lactose, maltodextrin). On the other hand casein at a concentration of 0.5% (w/v) was the selected nitrogen source in the media formulation. Under optimized conditions enzyme levels reached 130 SU per ml fermentation broth. The inoculum type and size has also affected biomass production and the biosynthesis of the enzyme. The preferred method was the inoculation of the culture media with spores at a total load of 6x105 spores per flask.Key words: Milk clotting enzyme, Aspartic protease, Mucor mucedo, Sub-merged fermentation

Decolorization and partial mineralization of a polyazo dye by Bacillus firmus immobilized within tubular polymeric gel

The degradation of C.I. Direct red 80, a polyazo dye, was investigated using Bacillus firmus immobilized by entrapment in tubular polymeric gel. This bacterial strain was able to completely decolorize 50 mg/L of C.I. Direct red 80 under anoxic conditions within 12 h and also degrade the reaction intermediates (aromatic amines) during the subsequent 12 h under aerobic conditions. The tubular gel harboring the immobilized cells consisted of anoxic and aerobic regions integrated in a single unit which was ideal for azo dye degradation studies. Results obtained show that effective dye decolorization (97.8%), chemical oxygen demand (COD) reduction (91.7%) and total aromatic amines removal were obtained in 15 h with the immobilized bacterial cell system whereas for the free cells, a hydraulic residence time of 24 h was required for an equivalent performance in a sequential anoxic and aerobic process. Repeated-batch experiments indicate the immobilized cells could decolorize C.I. Direct red 80 and reduce medium COD in five successive batch runs with enhanced activity obtained after each consecutive run, thus suggesting its stability and potential for repeated use in wastewater treatment. UV–visible spectrophotometry and HPLC analysis were used to confirm the partial mineralization of the dye. Data from this study could be used as a reference for the development of effective industrial scale biotechnological process for the removal of dyes and their metabolites in textile wastewater

CARBOHYDRATE POLYMERS

The production of pullulan from hydrolysed potato starch waste by Aureobasidium pullulans P56 was investigated. The liquefaction of potato starch was done by Ca-alginate immobilized amyloglucosidase and pullulanase enzymes in a packed bed bioreactor. Various organic nitrogen sources were tested and none of the nitrogen sources gave pullulan concentrations as high as that obtained with yeast extract. Response surface methodology was used to investigate the effects of three factors (incubation time, initial substrate concentration and initial pH) on the concentration of pullulan in batch cultures of A. pullulans. No previous work has used statistical analysis on the optimization of process parameters in pullulan production from hydrolysed potato starch waste. Maximum pullulan concentration of 19.2 g/l was obtained at the optimum levels of process variables (incubation time 111.8 h, initial substrate concentration 79.4 g/l, initial pH 7.26). The optimization led to a 20% increase in pullulan concentration. (C) 2010 Elsevier Ltd. All rights reserved

Production of lactic acid from beet molasses by calcium alginate immobilized Lactobacillus delbrueckii IFO 3202

WOS: 000078820500005Lactic acid was produced from pretreated beet molasses by the homofermentative organism Lactobacillus delbrueckii subsp delbrueckii IFO 3202 entrapped in calcium alginate gel using batch, repeated batch and continuous fermentation systems. In batch fermentation studies successful results were obtained with 2.0-2.4 mm diameter beads prepared from 2% sodium alginate solution. The highest effective yield (82.0%) and conversion yield (90.0%) were obtained from substrate concentrations of 52.1 and 78.2 g dm(-3) respectively. The gel beads produced lactic acid for 14 consecutive batch fermentations without marked activity loss and deformation. In the continuous fermentation, the highest lactic acid (4.22%) was obtained at a dilution rate of 0.1 h(-1) while the highest productivity (13.92 g dm(-3) h(-1)) was obtained at a dilution rate of 0.4 h(-1). (C) 1999 Society of Chemical Industry

Natural red pigment production by monascus purpureus in submerged fermentation systems using a food industry waste: Brewer's spent grain

This paper studies the production of natural red pigments by Monascus purpureus CMU001 in the submerged fermentation system using a brewery waste hydrolysate, brewer's spent grain (BSG). The chemical, structural and elemental characterization of the BSG was performed with Van-Soest method, Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), respectively. The lignocellulosic structure of BSG was hydrolyzed with a dilute sulfuric acid solution (2% (w/v)) followed by detoxification with Ca(OH)2. Maximum red pigment production (22.25 UA500) was achieved with the following conditions: 350 rpm shake speed, 50 mL fermentation volume, initial pH of 6.5, inoculation ratio of 2% (v/v), and monosodium glutamate (MSG) as the most effective nitrogen source. Plackett-Burman design was used to assess the significance of the fermentation medium components, and MSG and ZnSO47H2O were found to be the significant medium variables. This study is the first study showing the compatibility of BSG hydrolysate to red pigment production by Monascus purpureus in a submerged fermentation system. © 2019 MDPI Multidisciplinary Digital Publishing Institute. All rights reserved.Ege Üniversitesi: 16-MÜH-031 16-MUH-031ammonia, which increased the pH of the fermentation broth. Author Contributions: Conceptualization, Y.G.; Methodology, S.S. and Y.G.; Validation, Y.G. and S.S.; Data Curation, S.S. and Y.G.; Writing-Original Draft Preparation, S.S.; Writing-Review and Editing, Y.G. 4. Conclusions Funding: This research was funded by the EGE UNIVERSITY SCIENTIFIC RESEARCH PROJECT FOUNDATION, 16-MÜH-031.Monascus pigments have received worldwide attention for their multiple health benefits; they appear to have anti-mutagenic, anti-cancer, anti-obesity, anti-inflammation, anti-diabetes, and Acknowledgments: We appreciate Ege University Scientific Research Project Fund (Project No: 16-MUH-031) cholesterol-lowering mechanisms. This research article is the first on the evaluation of hydrolyzed for supporting this research. The researchers are thankful to Professor Saisamorn Lumyong for providing the and detoxified brewer’s spent grain for red pigment production by Monascus purpureus in submerged Perendeci and Laboratory of Environmental Biotechnology of Akdeniz University for opening their laboratory and supporting the Van Soest analysis and Huriye Goksungur for proofreading the manuscript. -