End to End Deep Neural Network Frequency Demodulation of Speech Signals

Frequency modulation (FM) is a form of radio broadcasting which is widely used nowadays and has been for almost a century. We suggest a software-defined-radio (SDR) receiver for FM demodulation that adopts an end-to-end learning based approach and utilizes the prior information of transmitted speech message in the demodulation process. The receiver detects and enhances speech from the in-phase and quadrature components of its base band version. The new system yields high performance detection for both acoustical disturbances, and communication channel noise and is foreseen to out-perform the established methods for low signal to noise ratio (SNR) conditions in both mean square error and in perceptual evaluation of speech quality score

Drying and cracking mechanisms in a starch slurry

Starch-water slurries are commonly used to study fracture dynamics. Drying starch-cakes benefit from being simple, economical, and reproducible systems, and have been used to model desiccation fracture in soils, thin film fracture in paint, and columnar joints in lava. In this paper, the physical properties of starch-water mixtures are studied, and used to interpret and develop a multiphase transport model of drying. Starch-cakes are observed to have a nonlinear elastic modulus, and a desiccation strain that is comparable to that generated by their maximum achievable capillary pressure. It is shown that a large material porosity is divided between pore spaces between starch grains, and pores within starch grains. This division of pore space leads to two distinct drying regimes, controlled by liquid and vapor transport of water, respectively. The relatively unique ability for drying starch to generate columnar fracture patterns is shown to be linked to the unusually strong separation of these two transport mechanisms.Comment: 9 pages, 8 figures [revised in response to reviewer comments

Drying and cracking mechanisms in a starch slurry

Starch-water slurries are commonly used to study fracture dynamics. Drying starch-cakes benefit from being simple, economical, and reproducible systems, and have been used to model desiccation fracture in soils, thin film fracture in paint, and columnar joints in lava. In this paper, the physical properties of starch-water mixtures are studied, and used to interpret and develop a multiphase transport model of drying. Starch-cakes are observed to have a nonlinear elastic modulus, and a desiccation strain that is comparable to that generated by their maximum achievable capillary pressure. It is shown that a large material porosity is divided between pore spaces between starch grains, and pores within starch grains. This division of pore space leads to two distinct drying regimes, controlled by liquid and vapor transport of water, respectively. The relatively unique ability for drying starch to generate columnar fracture patterns is shown to be linked to the unusually strong separation of these two transport mechanisms.Comment: 9 pages, 8 figures [revised in response to reviewer comments

Controlling the drying-induced peeling of colloidal films

In this work, we investigated the effect of the suspension properties on the drying dynamics and the resulting film peeling instability. To do so, a comprehensive series of experiments were conducted using drops of aqueous mixtures of colloidal silica dispersions and polyethylene oxide (PEO) additives. Time- lapse digital microscope images of the evaporating droplets show that film peeling can be discouraged and eventually eliminated with an increase in PEO concentration and molecular weight. This is due to the additives modifying the suspension properties which in turn modify the drying front length across the evaporating surface. Our result extends the understanding of the physics of film failure which is relevant information for various industrial processes such as in inkjet printing and coating applications

Fundamental investigation of the drying of solid suspensions

In this work, a comprehensive series of experiments is conducted to investigate the drying behaviour of micro- and nano-sized particle dispersions. To this end, an acoustic levitator was used to study the drying kinetics of single droplets. The temporal evolution of the actual droplets was recorded using a CMOS camera and the solid grains produced at the end of drying were investigated by SEM imaging. At the end of drying, the grains show different morphologies as a function of the particle size, concentration and initial droplet volume. We combine these experimental data to show the drying behaviour is dependent on all the parameters and that the data all collapses when plotted against Péclet number. This resulted in a novel characteristic diagram which allows one to predict the shape of the dried colloidal droplet based on Pé. Our results extend the fundamental understanding of the mechanisms controlling drying of droplet suspensions

Regulated Activation of the PAR Polarity Network Ensures a Timely and Specific Response to Spatial Cues

How do cells polarize at the correct time and in response to the correct cues? In the C. elegans zygote, the timing and geometry of polarization rely on a single dominant cue-the sperm centrosome-that matures at the end of meiosis and specifies the nascent posterior. Polarization requires that the conserved PAR proteins, which specify polarity in the zygote, be poised to respond to the centrosome. Yet, how and when PAR proteins achieve this unpolarized, but responsive, state is unknown. We show that oocyte maturation initiates a fertilization-independent PAR activation program. PAR proteins are initially not competent to polarize but gradually acquire this ability following oocyte maturation. Surprisingly, this program allows symmetry breaking even in unfertilized oocytes lacking centrosomes. Thus, if PAR proteins can respond to multiple polarizing cues, how is specificity for the centrosome achieved? Specificity is enforced by Polo-like and Aurora kinases (PLK-1 and AIR-1 in C. elegans), which impose a delay in the activation of the PAR network so that it coincides with maturation of the centrosome cue. This delay suppresses polarization by non-centrosomal cues, which can otherwise trigger premature polarization and multiple or reversed polarity domains. Taken together, these findings identify a regulatory program that enforces proper polarization by synchronizing PAR network activation with cell cycle progression, thereby ensuring that PAR proteins respond specifically to the correct cue. Temporal control of polarity network activity is likely to be a common strategy to ensure robust, dynamic, and specific polarization in response to developmentally deployed cues

Anterior-enriched filopodia create appearance of asymmetric membrane microdomains in polarizing C. elegans zygotes

The association of molecules within membrane microdomains is critical for the intracellular organization of cells. During polarization of the C. elegans zygote, both polarity proteins and actomyosin regulators associate within dynamic membrane-associated foci. Recently, a novel class of asymmetric PIP2 membrane-associated structures was described, suggesting that PIP2 domains could constitute signaling hubs to promote cell polarization and actin nucleation. Here we probe the nature of these domains using a variety of membrane- and actin cortex-associated probes. These data demonstrate that these domains are filopodia, which are stimulated transiently during polarity establishment and accumulate in the zygote anterior. The resulting membrane protrusions create local membrane topology that quantitatively accounts for observed local increases in fluorescence signal of membrane-associated molecules, suggesting molecules are not selectively enriched in these domains relative to bulk membrane and that the PIP2 pool as revealed by PHPLCδ1 simply reflects plasma membrane localization. Given the ubiquity of 3D membrane structures in cells, including filopodia, microvilli, and membrane folds, similar caveats are likely to apply to analysis of membrane-associated molecules in a broad range of systems

Effects of dried distillers grains with solubles on sow carcass fat quality

A pilot experiment was conducted to determine the effects of feeding nonpregnant (open) sows a diet containing 50% dried distillers grains with solubles (DDGS) on growth and carcass fat quality. A total of 8 open sows were allotted to 1 of 2 diets by parity and BW. One diet was a standard corn-soybean meal-based gestation diet; the second diet was a corn-soybean meal-based diet that contained 50% DDGS. All sows were fed 5 lb/d of feed in a single feeding for 92 d. All sows were harvested on d 92 at the Kansas State University Meat Laboratory for determination of carcass fat quality. As expected, no differences in BW or backfat change were found (P \u3e 0.62) for the feeding period. Additionally, no differences (P \u3e 0.23) in lipid oxidation as measured by 2-thiobarbituric acid reactive substances (TBARS) assay were reported either initially or after 5 d of retail display for sows fed 50% DDGS compared with controls. Lipid oxidation increased (P \u3c 0.003) as measured by TBARS assay for both treatments from d 1 to 5 as expected. Jowl fatty acid analysis revealed an increase in linoleic acid (P \u3c 0.01), total polyunsaturated fatty acids (P \u3c 0.01), and the ratio of polyunsaturated fatty acids to saturated fatty acids (P \u3c 0.03). Also, there was a trend for increased jowl iodine value (P \u3c 0.08) for sows fed 50% DDGS compared with the controls. In summary, feeding 50% DDGS to open sows for 92 d did not significantly affect BW, backfat, and lipid oxidation compared with controls. However, feeding 50% DDGS increased the concentration of linoleic acid and total polyunsaturated fatty acids and tended to increase jowl iodine value compared with controls.; Swine Day, 2008, Kansas State University, Manhattan, KS, 200

Mechanochemical feedback regulates the dynamics of the PAR system in C. elegans zygotes

The interplay between regulatory biochemistry and cell mechanics is critical for a broad range of morphogenetic changes. Cell mechanics can induce transport via growth and flow-fields, which in turn affect concentration-fields of regulators. Such systems exhibit an intrinsic feedback-architecture between regulators of cell mechanics and mechanical deformation. While we anticipate that this feedback between biochemistry and cell mechanics is widespread in Morphogenesis, there are few examples that are studied with respect to their potential for generating spatiotemporal patterns. Here we establish at a quantitative level that PAR polarization of C. elegans zygotes represents a coupled mechanochemical system. Using Fluorescence Recovery After Photobleaching (FRAP) and RNA interference (RNAi), we first demonstrate that the biochemistry in form of the PAR domains feeds back on the mechanics by establishing and maintaining a non-muscle myosin II (nmy-2) gradient. Additionally, we characterize the effect of the polarity cue associated with the centrosome of the male pronucleus on the local myosin concentration at the posterior pole. We show that it induces a reduction in myosin concentration and thereby triggers the onset of cortical flows. Furthermore we measure the spatiotemporal profile of the anterior and posterior PAR concentration, the myosin II concentration and the induced flow-field. Finally, we capture the feedback-architecture of the coupled actomyosin – PAR system in a quantitative model, based on coupling a thin film active fluid description of cortical mechanics [1] to a reaction-diffusion PAR patterning system [2]. We show that this mathematical model can quantitatively recapitulate the spatiotemporal profile of PAR polarity establishment. Furthermore, we demonstrate that the model predicts the existence of a threshold in cortical flow velocity, which separates the nonpolarizing and the polarizing regime and confirm the existence of this threshold velocity in the living C. elegans zygote

Novel Role of Phosphorylation-Dependent Interaction between FtsZ and FipA in Mycobacterial Cell Division

The bacterial divisome is a multiprotein complex. Specific protein-protein interactions specify whether cell division occurs optimally, or whether division is arrested. Little is known about these protein-protein interactions and their regulation in mycobacteria. We have investigated the interrelationship between the products of the Mycobacterium tuberculosis gene cluster Rv0014c-Rv0019c, namely PknA (encoded by Rv0014c) and FtsZ-interacting protein A, FipA (encoded by Rv0019c) and the products of the division cell wall (dcw) cluster, namely FtsZ and FtsQ. M. smegmatis strains depleted in components of the two gene clusters have been complemented with orthologs of the respective genes of M. tuberculosis. Here we identify FipA as an interacting partner of FtsZ and FtsQ and establish that PknA-dependent phosphorylation of FipA on T77 and FtsZ on T343 is required for cell division under oxidative stress. A fipA knockout strain of M. smegmatis is less capable of withstanding oxidative stress than the wild type and showed elongation of cells due to a defect in septum formation. Localization of FtsQ, FtsZ and FipA at mid-cell was also compromised. Growth and survival defects under oxidative stress could be functionally complemented by fipA of M. tuberculosis but not its T77A mutant. Merodiploid strains of M. smegmatis expressing the FtsZ(T343A) showed inhibition of FtsZ-FipA interaction and Z ring formation under oxidative stress. Knockdown of FipA led to elongation of M. tuberculosis cells grown in macrophages and reduced intramacrophage growth. These data reveal a novel role of phosphorylation-dependent protein-protein interactions involving FipA, in the sustenance of mycobacterial cell division under oxidative stress