Endodontic Rotary Systems : Comparison between gentlefile and pro taper universal for removal of enterococcus faecalis

As a resistant bacterium species in infected root canals, Enterococcus faecalis needs to be removed in any endodontic treatment. So, we aimed to compare the effectiveness of two rotary systems, Gentlefile and Pro Taper Universal, in removing Enterococcu

Apical Microleakage in Root Canals Containing Broken Rotary Instruments

Introduction: Broken instruments in root canals complicate routine endodontic treatment. This study aimed to compare apical microleakage in root canals containing broken rotary instruments filled with mineral trioxide aggregate (MTA), calcium-enriched mixture (CEM) cement, laterally compacted gutta-percha and injected gutta-percha. Methods and Materials: In this in vitro, experimental study, 80 extracted human premolars were decoronated and then the roots were randomly divided into four groups (n=20). Root canals were instrumented with Mtwo rotary files. The files were scratched 3 mm from the tip by a high speed handpiece and they were intentionally broken in the apical third of the canals. The middle and coronal thirds of the canals were then filled with MTA, CEM cement, gutta-percha with lateral compaction technique and injected gutta-percha. Apical microleakage was measured using dye penetration method. Data were analyzed using ANOVA and Tukey’s test. Results: Root canals filled with CEM cement showed the lowest and those filled with injected gutta-percha showed the highest microleakage according to dye penetration depth. No significant difference was noted between the microleakage of CEM cement and MTA or between lateral compaction of gutta-percha and injected gutta-percha (P>0.05). However, CEM cement and MTA groups had significantly lower microleakage than laterally compacted and injected gutta-percha groups (P<0.05). Conclusion: Due to their superior sealing ability, MTA and CEM cement are suitable for filling of root canals containing a broken instrument compared to laterally compacted and injected gutta-percha.Keywords: Broken Instrument; Calcium-Enriched Mixture; Gutta-Percha; Leakage; Mineral Trioxide Aggregate; Root Canal Filling Material

Simulated Evaluation of Tooth Fracture Resistance during Instrumentation with Single-and Multi-file Rotary Systems

Introduction: Use of rotary files in root canal preparation is inevitable due to their high speed and reduced duration of treatment. However, microcracks and even root fracture may sometimes occur in teeth where canal(s) is/are prepared using these devices. The purpose of the current study was to compare NeoNiti single-file with RaCe multi-file rotary systems. Materials and Methods: An accurate scanner designed detailed three-dimensional file models, and a simulated model was developed using canal-specific features; including dentin thickness, root canal length, taper rate and root canal curvature. Cleaning and shaping conditions were simulated with appropriate speed and crack conditions for both types of files on the computer. Strain accumulation sites in the root dentin wall where microcracks was likely to occur were simulated and investigated. Abaqus and ANSYS software were used to perform finite element simulations. Results: RaCe files with 0.04 taper caused the lowest level of stress (34.33 MPa) to the simulated canal. Conversely, the highest level of stress (62.35 MPa) was applied by 8% NeoNiti file to the simulated canal. Conclusions: Based on the present experimental study, it can be concluded that RaCe multi-file rotary system may better be used in endodontic therapy, and the risk of microcracks in the tooth wall is less than that of NeoNiti single-file rotary system

Comparison of the Antibacterial Effect of 810 nm Diode Laser and Photodynamic Therapy in Reducing the Microbial Flora of Root Canal in Endodontic Retreatment in Patients With Periradicular Lesions

Introduction: The aim of this study was to compare the antibacterial efficacy of diode laser 810nm and photodynamic therapy (PDT) in reducing bacterial microflora in endodontic retreatment of teeth with periradicular lesion.Methods: In this in vivo clinical trial, 20 patients who needed endodontic retreatment were selected. After conventional chemo mechanical preparation of root canals, microbiological samples were taken with sterile paper point (PP), held in thioglycollate broth, and then were transferred to the microbiological lab. In the first group, PDT with methylene blue (MB) and diode laser (810 nm, 0.2 W, 40 seconds) was performed and in the second group diode laser (810 nm, 1.2 W, 30 seconds) was irradiated. Then second samples were taken from all canals.Results: CFU/ml amounts showed statistically significant reduction in both groups (P < 0.001). CFU/ml amounts were compared between the two groups and there was no statistical difference.Conclusion: PDT and diode laser 810 nm irradiation are effective methods for root canal disinfection. PDT is a suitable alternative for diode laser 810 nm irradiation, because of lower thermal risk on root dentin

Effect of Topical Application of Dentol Drop on the Success of Inferior Alveolar Nerve Block for Teeth with Irreversible Pulpitis: A Double-blind Randomized Clinical Trial

Introduction: The failure rate of inferior alveolar nerve (IAN) block is high for mandibular molars with irreversible pulpitis. This double-blind, randomized, clinical trial aimed to assess the effect of topical application of Dentol drop on the rate of successful anaesthesia of mandibular molars with irreversible pulpitis due to deep carious lesions. Methods and Materials: Seventy-two patients with mandibular first and second molars with irreversible pulpitis and deep cavitated carious lesions participated in this study. The patients were randomly assigned to the test and control groups (n=36). In the test group, a cotton pellet, dipped in Dentol drop, was placed in the cavity for 10 min. A placebo was used _in the same manner_ in the control group. Level of pain was measured before the intervention, 15 min after anaesthesia (when patients reported numbness at the corner of the mouth), during access cavity preparation, upon pulp exposure and after introduction of the initial file into the root canal; using a Heft-Parker “Visual Analog Scale” (VAS). Data were analysed using ANCOVA. Results: Levels of pain were recorded during access cavity preparation (P<0.001), pulp exposure (P<0.001) and file introduction into the canal (P=0.018). In the test (Dentol) group, the obtained levels of pain were significantly lower than those of the corresponding values in the control group. Conclusion: Topical application of Dentol drop increased the success rate of IAN block for root canal treatment of mandibular molars with irreversible pulpitis.Keywords: Dentol Drop; Inferior Alveolar Nerve Block; Irreversible Pulpiti

Association between IL-8 (-251T/A) and IL-6 (-174G/C) Polymorphisms and Oral Cancer Susceptibility: A Systematic Review and Meta-Analysis

Background and objective: Inflammation and cell-mediated immunity can have significant roles in different stages of carcinogenesis. The present meta-analysis aimed to evaluate the association between the polymorphisms of IL-8 (-251T/A) and IL-6 (-174G/C) and the risk of oral cancer (OC). Methods: PubMed/MEDLINE, Web of Science, Cochrane Library, and Scopus databases were searched until December 18, 2020 without any restrictions. RevMan 5.3 software was used to calculate the results of forest plots (odds ratios (ORs) and 95% confidence intervals (CIs)); CMA 2.0 software was used to calculate funnel plots (Begg’s and Egger’s tests), and SPSS 22.0 was used for the meta-regression analysis. Moreover, trial sequential analysis was conducted to estimate the robustness of the results. Results: Eleven articles including twelve studies were selected for the meta-analysis. The pooled ORs for the association between IL-8 (-251T/A) polymorphism and the risk of OC in the models of A vs. T, AA vs. TT, TA vs. TT, AA + TA vs. TT, and AA vs. TT + TA were 0.97 (p = 0.78), 0.86 (p = 0.55), 0.78 (p = 0.37), 0.83 (p = 0.45), and 1.10 (p = 0.34), respectively. The pooled ORs IL-6 (-174G/C) polymorphism and the risk of OC in the models of C vs. G, CC vs. GG, GC vs. GG, CC + GC vs. GG, and CC vs. GG + GC were 1.07 (p = 0.87), 1.17 (p = 0.82), 1.44 (p = 0.38), 1.28 (p = 0.61), and 0.96 (p = 0.93), respectively. There was no association between IL-8 (-251T/A) polymorphism and OC susceptibility, but the C allele and GC and CC genotypes of IL-6 (-174G/C) polymorphism were associated with the risk of OC based on subgroup analyses, that is to say, the source of control and the genotyping method might bias the pattern of association. Conclusions: The meta-analysis confirmed that there was no association between the polymorphisms of IL-6 (-174G/C) and IL-8 (-251T/A) and the susceptibility of OC. However, the source of control and the genotyping method could unfavorably impact on the association between the polymorphisms of IL-6 (-174G/C) and the risk OC

In vitro evaluation of coronal discoloration following the application of calcium-enriched mixture cement, Biodentine, and mineral trioxide aggregate in endodontically treated teeth

Background: This study sought to assess and compare coronal discoloration following the application of white mineral trioxide aggregate, Biodentine, and calcium-enriched mixture cement in endodontically treated teeth. Materials and Methods: In this in vitro experimental study, 64 freshly extracted sound human premolars were selected, cleaned, and stored in saline. After cleaning, shaping, and obturation the root canal of the teeth, the teeth were randomly assigned to one control (n = 4) and three experimental (n = 20) groups. In the experimental groups, the cement were applied over the canal orifices in 3-mm thickness. All teeth were then restored with composite resin. Color parameters, according to the CIE LFNx01aFNx01bFNx01 system, were measured using Vita Easyshade spectrophotometer before application of cement and at 1 week, 1 month, 2 months, and 3 months after the application of cement. The recorded values were statistically analyzed using descriptive and analytical statistics. For analytical statistics, Kolmogorov–Smirnov test was applied to assess normal distribution of data. ANOVA was used to compare the results at baseline and repeated measures. P < 0.05 was considered statistically significant Results: Significant differences were noted in color change (ΔE) between all time points except between ΔE4 (2 months) and ΔE5 (3 months) (P < 0.01). However, tooth discoloration caused by the three cement was not significantly different (P = 0.343). Conclusion: The three tested cement were not significantly different in terms of causing coronal discoloration in endodontically treated teeth

Association between IL-8 (-251T/A) and IL-6 (-174G/C) Polymorphisms and Oral Cancer Susceptibility: A Systematic Review and Meta-Analysis

Inflammation and cell-mediated immunity can have significant roles in different stages of carcinogenesis. The present meta-analysis aimed to evaluate the association between the polymorphisms of; IL-8 (-251T/A); and; IL-6 (-174G/C); and the risk of oral cancer (OC).; PubMed/MEDLINE, Web of Science, Cochrane Library, and Scopus databases were searched until December 18, 2020 without any restrictions. RevMan 5.3 software was used to calculate the results of forest plots (odds ratios (ORs) and 95% confidence intervals (CIs)); CMA 2.0 software was used to calculate funnel plots (Begg's and Egger's tests), and SPSS 22.0 was used for the meta-regression analysis. Moreover, trial sequential analysis was conducted to estimate the robustness of the results.; Eleven articles including twelve studies were selected for the meta-analysis. The pooled ORs for the association between; IL-8 (-251T/A); polymorphism and the risk of OC in the models of A vs. T, AA vs. TT, TA vs. TT, AA + TA vs. TT, and AA vs. TT + TA were 0.97 (; p; = 0.78), 0.86 (; p; = 0.55), 0.78 (; p; = 0.37), 0.83 (; p; = 0.45), and 1.10 (; p; = 0.34), respectively. The pooled ORs; IL-6 (-174G/C); polymorphism and the risk of OC in the models of C vs. G, CC vs. GG, GC vs. GG, CC + GC vs. GG, and CC vs. GG + GC were 1.07 (; p; = 0.87), 1.17 (; p; = 0.82), 1.44 (; p; = 0.38), 1.28 (; p; = 0.61), and 0.96 (; p; = 0.93), respectively. There was no association between; IL-8 (-251T/A); polymorphism and OC susceptibility, but the C allele and GC and CC genotypes of; IL-6 (-174G/C); polymorphism were associated with the risk of OC based on subgroup analyses, that is to say, the source of control and the genotyping method might bias the pattern of association.; The meta-analysis confirmed that there was no association between the polymorphisms of; IL-6 (-174G/C); and; IL-8 (-251T/A); and the susceptibility of OC. However, the source of control and the genotyping method could unfavorably impact on the association between the polymorphisms of; IL-6 (-174G/C); and the risk OC