Effects of sub-lethal single, simultaneous, and sequential abiotic stresses on phenotypic traits of Arabidopsis thaliana

Plant responses to abiotic stresses are complex and dynamic, and involve changes in different traits, either as the direct consequence of the stress, or as an active acclimatory response. Abiotic stresses frequently occur simultaneously or in succession, rather than in isolation. Despite this, most studies have focused on a single stress and single or few plant traits. To address this gap, our study comprehensively and categorically quantified the individual and combined effects of three major abiotic stresses associated with climate change (flooding, progressive drought and high temperature) on 12 phenotypic traits related to morphology, development, growth and fitness, at different developmental stages in four Arabidopsis thaliana accessions. Combined sub-lethal stresses were applied either simultaneously (high temperature and drought) or sequentially (flooding followed by drought). In total, we analyzed the phenotypic responses of 1782 individuals across these stresses and different developmental stages. Overall, abiotic stresses and their combinations resulted in distinct patterns of effects across the traits analyzed, with both quantitative and qualitative differences across accessions. Stress combinations had additive effects on some traits, whereas clear positive and negative interactions were observed for other traits: 9 out of 12 traits for high temperature and drought, 6 out of 12 traits for post-submergence and drought showed significant interactions. In many cases where the stresses interacted, the strength of interactions varied across accessions. Hence, our results indicated a general pattern of response in most phenotypic traits to the different stresses and stress combinations, but it also indicated a natural genetic variation in the strength of these responses. Overall, our study provides a rich characterization of trait responses of Arabidopsis plants to sub-lethal abiotic stresses at the phenotypic level and can serve as starting point for further in-depth physiological research and plant modelling efforts

Effects of sublethal single, simultaneous and sequential abiotic stresses on phenotypic traits of Arabidopsis thaliana

Plant responses to abiotic stresses are complex and dynamic, and involve changes in different traits, either as the direct consequence of the stress, or as an active acclimatory response. Abiotic stresses frequently occur simultaneously or in succession, rather than in isolation. Despite this, most studies have focused on a single stress and single or few plant traits. To address this gap, our study comprehensively and categorically quantified the individual and combined effects of three major abiotic stresses associated with climate change (flooding, progressive drought and high temperature) on 12 phenotypic traits related to morphology, development, growth and fitness, at different developmental stages in four Arabidopsis thaliana accessions. Combined sublethal stresses were applied either simultaneously (high temperature and drought) or sequentially (flooding followed by drought). In total, we analysed the phenotypic responses of 1782 individuals across these stresses and different developmental stages. Overall, abiotic stresses and their combinations resulted in distinct patterns of effects across the traits analysed, with both quantitative and qualitative differences across accessions. Stress combinations had additive effects on some traits, whereas clear positive and negative interactions were observed for other traits: 9 out of 12 traits for high temperature and drought, 6 out of 12 traits for post-submergence and drought showed significant interactions. In many cases where the stresses interacted, the strength of interactions varied across accessions. Hence, our results indicated a general pattern of response in most phenotypic traits to the different stresses and stress combinations, but it also indicated a natural genetic variation in the strength of these responses. This includes novel results regarding the lack of a response to drought after submergence and a decoupling between leaf number and flowering time after submergence. Overall, our study provides a rich characterization of trait responses of Arabidopsis plants to sublethal abiotic stresses at the phenotypic level and can serve as starting point for further in-depth physiological research and plant modelling efforts

The legal, historical and industrial context of underwater heritage:Introduction

Interactions between commercial and industrial exploitation of the seabed and archaeological and scientific investigation have been at the heart of developments in the understanding of Europe’s submerged landscapes and prehistory since at least the early twentieth century. This introduction considers some of the ways in which that relationship has evolved since that time, including the adoption of international laws under the aegis of United Nations Conventions, the development of close relationships between Dutch fishermen operating beam-trawl fishing nets in the North Sea and a network of private collectors specialising in Pleistocene fossils and artefacts, the imposition of European Union regulations on offshore industrial projects to include monitoring of underwater archaeology and palaeoenvironments and most recently the incorporation of seabed mapping and underwater cultural heritage in the European Union’s 2020 Blue Growth agenda. These developments have played an important role in the growth of knowledge about the underwater cultural heritage notwithstanding the potentially destructive effects of offshore industrial activity. The impact of economic growth and industrial exploitation in the coastal zone, coupled with sea-level rise, is likely to intensify the threats to the underwater cultural heritage in the coming decades, posing new challenges as well as opportunities in the further development of relationships between industrial operators, government agencies and scientific and archaeological researchers

Kaart van den loop der rivieren de Rhijn, de Maas, de Waal, de Merwede, de Lek, en de Linge, waarop alle door die rivieren veroorzaakte belangrijke dijkbreuken, alsmede de zich in dezelve vast gezet hebbende ijsdammen, sedert de XV. eeuw, met het jaartal zijn aangeduid

Nog een typisch voorbeeld van pre-lithografische thematische kaarten, waarop de thematische informatie met de hand werd ingekleurd en daardoor pas ook werkelijk opviel. Op deze kaart (zowel west- als oostblad zijn opgenomen) zijn alle dijkdoorbraken met een rode kleur aangegeven, naast het jaartal. Alleen in de legenda is die rode kleur bij de dijkdoorbraken niet ook ingekleurd, wat natuurlijk wél had gemoeten. Tevens toegevoegd zijn gekleurde biezen die de verschillende provincies begrenzen: geel voor Gelderland, blauw voor (Noord-)Brabant, oranje voor Zuid-Holland en paars voor Utrecht. Het is ook in dìe zin een vroege thematische kaart dat er met symbolen gebeurtenissen of verschijnselen gekenmerkt worden, zonder dat er van groepering of karakterisering sprake is

Psychopathy, maladaptive learning and risk taking

Individuals with psychopathy present with maladaptive tendencies that have been linked to disturbed processing of outcomes during decision making, in particular with respect to aversive outcomes. In general, individuals with psychopathy show risk-seeking behaviour, as well as excessive reward-oriented behaviour. This chapter provides an overview of theories and empirical work on maladaptive behaviour in psychopathy in the context of reinforcement learning and risky decision making. In addition, we capitalise on recent neuroscientific advances to discuss the empirical results and propose how the use of personalised rewards in experimental designs may help to create a more ecologically valid reflection of real-world maladaptive behaviour in psychopathy

Relevance of somatosensory evoked potential amplitude after cardiac arrest

Contains fulltext : 220543.pdf (publisher's version ) (Open Access)Objective: We present relations of SSEP amplitude with neurological outcome and of SSEP amplitude with EEG amplitude in comatose patients after cardiac arrest. Methods: This is a post hoc analysis of a prospective cohort study in comatose patients after cardiac arrest. Amplitude of SSEP recordings obtained within 48-72 h, and EEG patterns obtained at 12 and 24h after cardiac arrest were related to good (CPC 1-2) or poor (CPC 3-5) outcome at 6 months. In 39% of the study population multiple SSEP measurements were performed. Additionally, SSEP amplitude was related to mean EEG amplitude. Results: We included 138 patients (77% poor outcome). Absent SSEP responses, a N20 amplitude <0.4 μV within 48-72 h, and suppressed or synchronous EEG with suppressed background at 12 or 24 h after cardiac arrest were invariably associated with a poor outcome. Combined, these tests reached a sensitivity for prediction of poor outcome up to 58 at 100% specificity. N20 amplitude increased with a mean of 0.55 μV per day in patients with a poor outcome, and remained stable with a good outcome. There was no statistically significant correlation between SSEP and EEG amplitudes in 182 combined SSEP and EEG measurements (R (2) < 0.01). Conclusions: N20 amplitude <0.4 μV is invariably associated with poor outcome. There is no correlation between SSEP and EEG amplitude. Significance: SSEP amplitude analysis may contribute to outcome prediction after cardiac arrest