Relação da aterosclerose carotídea com mediadores inflamatórios e o óxido nítrico circulantes em pacientes ambulatoriais idosos do Distrito Federal

Trabalho de Conclusão de Curso (graduação)—Universidade de Brasília, Faculdade de Ceilândia, 2017.Introdução: As doenças cardiovasculares (DCV) ainda são consideradas a principal causa de morte no mundo. Tendo conhecimento de que o principal achado clínico associado ao desenvolvimento de uma DCV corresponde à aterosclerose, o presente estudo buscou avaliar a possível associação entre as variáveis clínicas, bioquímicas e inflamatórias com níveis circulantes totais de óxido nítrico (NOx) em pacientes idosos que não apresentavam nenhum quadro inflamatório grave ou doença com elevada progressão. Métodos: 168 indivíduos idosos participantes do estudo de coorte prospectivo denominado Prognóstico e Terapêutica em Geriatria (ProTeGer) tiveram traçados os seus perfis clínicos, bioquímicos, antropométricos e metabólicos, sem distinção de sexo, que seguiram os critérios de inclusão do estudo e assinaram termo de consentimento livre e esclarecido. Além disso, os níveis circulantes de 10 mediadores inflamatórios e de NO foram medidos. Por testes de correlação de Spearman ou Pearson, foram analisadas características categóricas ou contínuas (respectivamente) de acordo com NO sérico. Foi adotado P <0,005 como limite de significância conforme convenção de Bonferroni. Resultados: Apesar de não terem sido encontradas associações entre o óxido nítrico (NO) e variantes clínicas, bioquímicas e antropométricas, foi demonstrada uma correlação negativa entre as concentrações plasmáticas de NO e os níveis do mediador inflamatório Interleucina (IL)17a (r = -0,236; P = 0,004). Conclusão: Sendo o NOx um regulador das IL 17, uma interleucina inflamatória, infere-se que essa molécula gasosa corresponde a um fator protetor para aterosclerose, quando expresso em concentrações ideias.Introduction: Cardiovascular diseases (CVD) are still considered the leading cause of death in the world. Bearing in mind that the main clinical finding associated with the development of CVD is atherosclerosis, the present study sought to evaluate the possible association between clinical, biochemical and inflammatory variables with total circulating levels of nitric oxide (NOx) in elderly patients who did not present a severe inflammatory or disease progression. Methods: 168 elderly individuals enrolled in the prospective cohort study, Prognostic and Therapeutic in Geriatrics (ProTeGer), had their clinical, biochemical, anthropometric and metabolic profiles, without gender distinction, which followed the inclusion criteria of the study and signed a term of Free and informed consent. In addition, circulating levels of 10 inflammatory mediators and NO mediators were measured. Through the Spearman or Pearson correlation tests, categorical or continuous characteristics (respectively) were analyzed according to serum NO. P <.005 was adopted as the limit of significance according to the Bonferroni convention. Results: Although there were no associations between nitric oxide (NO) and clinical, biochemical and anthropometric variants, a negative correlation was observed between plasma NO concentrations and levels of the inflammatory mediator Interleukin (IL) 17a (r = -0.236; P = 0.004). Conclusion: Being the NOx a regulator of IL-17, an inflammatory interleukin, it is inferred that this gas molecule corresponds to a protective factor for atherosclerosis, when expressed in ideal concentrations

Análise de mediadores inflamatórios sistêmicos em pacientes portadores de câncer de próstata e de hiperplasia prostática benigna

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Medicina, Programa de Pós-Graduação em Ciências Médicas, 2020.A investigação do perfil inflamatório sistêmico de pacientes afetados por câncer de próstata (CaP) ou hiperplasia prostática (HPB) pode contribuir para caracterizar o perfil patológico, bem como permitir a identificação de marcadores e promover alternativas para tratamentos adequados e menos invasivos. Neste aspecto, este trabalho se propôs a comparar os níveis séricos de 10 mediadores inflamatórios clássicos em pacientes com 50 anos ou mais de idade afetados por CaP ou HPB. Para isso, foram considerados aspectos clínicos, bioquímicos, metabólicos, antropométricos e inflamatórios de cada paciente. A partir da análise estatística, foi encontrada uma correlação positiva fraca (d = 0,16) entre a IL-2 e os valores séricos de PSA total. Além disso, os valores séricos medianos de IL-2 foram três vezes maiores nos pacientes com CaP em comparação aos pacientes com HPB. Pela interpretação da literatura atual, levantamos a hipótese de que a atividade de macrófagos do tipo M1 infiltrados e células citotóxicas ativadas no meio neoplásico pode explicar esse aumento da IL-2 como parte de uma resposta antineoplásica endógena.Investigation on the systemic inflammatory profile of patients affected by prostate cancer (PCa) or prostatic hyperplasia (BPH) may contribute to characterize the pathological profile as well as enable identification of markers and promote alternatives for appropriate, less invasive treatments. This research compared the serum levels of 10 classic inflammatory mediators among patients aged 50 years or older affected by PCa or BPH. For this, clinical, biochemical, metabolic, anthropometric and inflammatory aspects of each patient were considered. From statistical analysis, a weak positive correlation (r = 0.16) between IL-2 with serum total PSA values was found. In addition, median serum IL-2 values were three times higher in patients with PCa compared to BPH patients. By interpretation of current literature, we hypothesize that the activity of infiltrated type M1 macrophages and activated cytotoxic cells in the neoplasm milieu might explain this increase of IL-2 as part of an endogenous anti-neoplastic response

Sleep profile, clinical variables, and working hours in elderly and middle-aged long-haul truck drivers

This cross-sectional study aimed to investigate the association of variables representative of the sociodemographic profile, working hours, and general health status of long-haul truck drivers with their reported sleep regimen. Questionnaires provided by the Transportation Social Service and the National Transportation Learning Service, in which data were collected from professional truckers aged &ge; 45 years, were analyzed. The results found allow us to infer that the sleep profile of these ground freight transportation workers seems to correlate negatively with general health (prevalence of arterial hypertension and drug use) and the number of working hours per day.</p

Serum Levels of Matrix Metalloproteinase-1 in Brazilian Patients with Benign Prostatic Hyperplasia or Prostate Cancer

Metalloproteinases (MMPs) are involved in metastatic tumor processes, with changes in circulating levels detected in several cancer types. Here, we compare serum concentrations of metalloproteinase-1 (MMP-1) across individuals clinically diagnosed with prostate cancer (PCa) or benign prostatic hyperplasia (BPH), correcting results for the rs495366 single nucleotide polymorphism (SNP) that predisposes to differential MMP-1 levels. 196 men aged ≥50 years were followed at a university hospital urology outpatient clinic, with clinical, anthropometric, and rectal examinations performed by one urologist. Blood samples obtained prior to any clinical intervention provided baseline MMP-1 and total/free PSA levels as well as metabolic, hormonal, and inflammatory markers. The SNP was genotyped by real-time PCR. Participants with medical and/or laboratory profile compatible with malignancy composed the PCa group when confirmed by the Gleason scale. As expected, A-allele homozygotes showed reduced levels of MMP-1. Genotype-adjusted analyses revealed the mean MMP-1 level as 2-fold higher in PCa carriers compared to BPH patients. No other differences were found according to the prostatic condition or genotypic distribution, except for the expected raise in total and free PSA levels in PCa. In conclusion, increased serum levels of MMP-1 were observed in this context of prostatic malignancy compared to a benign phenotype, regardless of a genetic influence