Intensified training increases salivary free light chains in trained cyclists:Indication that training volume increases oral inflammation

Periods of short-term intensified training (IT) are often used by athletes during training cycles over the season and undergoing phases of increased physical stress may impact upon the immune system. This study investigated the effects of a period of IT on free light chains (FLCs) in saliva - an emerging immune biomarker of oral inflammation - and matched serum samples in well-trained athletes. It also examined if IT influences basal FLC levels and FLC flux during acute exercise. Highly trained male cyclists (n = 10) underwent a 9-day period of IT; before and after IT participants performed a 1 h time trial (TT) on a cycle ergometer, with blood and saliva samples collected pre- and post-exercise. FLCs were assessed in serum and saliva, and IgG, IgA, IgM and creatinine were also measured in serum. Weekly training volume increased by 143% (95% CI 114–172%), p &lt; 0.001, during IT compared with pre-trial baseline training. Following IT, the cyclists demonstrated higher salivary FLC levels. Both salivary lambda FLC concentrations (p &lt; 0.05, η2 = 0.384) and secretion rates, and kappa FLC concentrations and secretion rates increased after IT. Salivary FLCs concentration and secretion rates decreased in response to the TT following IT (p &lt; 0.05, η2 = 0.387–0.428), but not in response to the TT prior to IT. No significant effects of IT on serum FLCs were observed. There were no significant changes in serum FLCs in response to the TT, before or after the IT period, nor did IT impact upon other serological responses to the TT. In conclusion, IT increased basal salivary FLC parameters and amplified decreases in salivary FLCs in response to acute exercise. Increases in salivary FLC concentration likely reflects alterations to oral inflammation during times of heavy training, and we show for the first time that FLCs may have utility as a marker of exercise stress and oral health status.</p

Turbulent diffusion and drift in galactic magnetic fields and the explanation of the knee in the cosmic ray spectrum

We reconsider the scenario in which the knee in the cosmic ray spectrum is explained as due to a change in the escape mechanism of cosmic rays from the Galaxy from one dominated by transverse diffusion to one dominated by drifts. We solve the diffusion equations adopting realistic galactic field models and using diffusion coefficients appropriate for strong turbulence (with a Kolmogorov spectrum of fluctuations) and consistent with the assumed magnetic fields. We show that properly taking into account these effects leads to a natural explanation of the knee in the spectrum, and a transition towards a heavier composition above the knee is predicted.Comment: 17 pp., 6 figures; revised version with minor changes. To appear in JHE

The Search for the Sidereal and Solar Diurnal Modulations in the Total MACRO Muon Data Set

We have analyzed 44.3M single muons collected by MACRO from 1991 through 2000 in 2,145 live days of operation. We have searched for the solar diurnal, apparent sidereal, and pseudo-sidereal modulation of the underground muon rate by computing hourly deviations of the muon rate from 6 month averages. We find evidence for statistically significant modulations with the solar diurnal and the sidereal periods. The amplitudes of these modulations are <0.1%, and are at the limit of the detector statistics. The pseudo-sidereal modulation is not statistically significant. The solar diurnal modulation is due to the daily atmospheric temperature variations at 20 km, the altitude of primary cosmic ray interactions with the atmosphere; MACRO is the deepest experiment to report this result. The sidereal modulation is in addition to the expected Compton-Getting modulation due to solar system motion relative to the Local Standard of Rest; it represents motion of the solar system with respect to the galactic cosmic rays toward the Perseus spiral arm.Comment: 18 pages, 8 of which are figures, 1 is a table. Accepted by Phys. Rev.

Longitudinal lung function assessment of patients hospitalised with COVID-19 using 1H and 129Xe lung MRI

BACKGROUND: Microvascular abnormalities and impaired gas transfer have been observed in patients with COVID-19. The progression of pulmonary changes in these patients remains unclear. RESEARCH QUESTION: Do patients hospitalised due to COVID-19 without evidence of architectural distortion on structural imaging show longitudinal improvements in lung function measured using 1H and 129Xe magnetic resonance imaging between 6-52 weeks after hospitalisation? STUDY DESIGN AND METHODS: Patients who were hospitalised due to COVID-19 pneumonia underwent a pulmonary 1H and 129Xe MRI protocol at 6, 12, 25 and 51 weeks after hospital admission in a prospective cohort study between 11/2020 and 02/2022. Imaging protocol: 1H ultra-short echo time, contrast enhanced lung perfusion, 129Xe ventilation, 129Xe diffusion weighted and 129Xe spectroscopic imaging of gas exchange. RESULTS: 9 patients were recruited (57±14 [median±interquartile range] years, 6/9 male). Patients underwent MRI at 6 (N=9), 12 (N=9), 25 (N=6) and 51 (N=8) weeks after hospital admission. Patients with signs of interstitial lung damage were excluded. At 6 weeks, patients demonstrated impaired 129Xe gas transfer (red blood cell to membrane fraction) but lung microstructure was not increased (apparent diffusion coefficient and mean acinar airway dimensions). Minor ventilation abnormalities present in four patients were largely resolved in the 6-25 week period. At 12 weeks, all patients with lung perfusion data (N=6) showed an increase in both pulmonary blood volume and flow when compared to 6 weeks, though this was not statistically significant. At 12 weeks, significant improvements in 129Xe gas transfer were observed compared to 6-week examinations, however 129Xe gas transfer remained abnormally low at weeks 12, 25 and 51. INTERPRETATION: 129Xe gas transfer was impaired up to one year after hospitalisation in patients who were hospitalised due to COVID-19 pneumonia, without evidence of architectural distortion on structural imaging, whereas lung ventilation wa normal at 52 weeks

Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen

Classical swine fever (CSF) is a highly contagious and severe viral disease of swine resulting in substantial production losses in different farming systems in many regions of the world. The accurate and rapid detection of CSF outbreaks is reliant on sensitive and specific laboratory testing and is a key component of disease control. Specific detection of CSF virus can be achieved by virus isolation in tissue culture, antigen capture or the detection of viral RNA using molecular techniques. In order to reduce the time taken to achieve a diagnostic result and simplify testing methods, an antigen capture ELISA using immunomagnetic beads (IMB) as the solid phase was developed and compared to a microplate-based antigen capture (AC)-ELISA. The IMB-ELISA has up to 64-fold greater analytical sensitivity than the AC-ELISA and initial estimates of diagnostic sensitivity and specificity are 100%. The IMB-ELISA has a highly robust, rapid and stable test format and is simpler to perform than the AC-ELISA. The IMB-ELISA has the added advantage that a result can be sensitively and specifically determined by eye, lending it to the possibility of adaptation to a near-to-field test with minimal equipment or expertise needed

An investigation of 11 outbreaks of foot-and-mouth disease in villages in northern Thailand

The results of investigations of 11 outbreaks of foot-and-mouth disease in villages in northern Thailand are described. The causative virus was Asia in one in seven outbreaks, Type O in two outbreaks and unknown in two outbreaks. The most probable sources of the outbreaks were co-mingling of cattle and/or buffalo with livestock from an infected neighbouring village (four) and recent introductions of infected cattle from a public livestock market (two) while the probable source could not be determined in five outbreaks. Attack rates in cattle and buffalo ranged from 0.28% to 50.9% but no pigs became sick during any of the outbreaks. Most outbreaks lasted 4 weeks or less. Adult cattle and buffalo were at higher risk of becoming a case when compared with work cattle. Beef cattle were at higher risk than buffalo and adult cattle and buffalo were at higher risk than calves less than 1 year of age. There was significant clustering of cases within households. Serological investigations indicated that many unaffected animals were probably not exposed to virus during the outbreaks. We concluded that close contact between animals was the main method of spread and that differences in attack rates between animal classes reflected differences in animal management. We further concluded that simple quarantine of early cases during outbreaks is likely to be effective in reducing spread within and between villages