Biochemical markers for the detection and classification of Aspergillus

The genus Aspergillus includes a diverse group of filamentous fungi that are widely distributed in nature, commonly found in soil. The Aspergilli include species that can be beneficial or detrimental to humans, so detection and accurate identification of these organisms can be very important. Morphology and genetic sequence analysis are well established methods for classifying and identifying fungi, but morphology remains a widely used technique that generally works well for Aspergilli. However, some organisms may be misidentified due to atypical morphology and some hidden (cryptic) species may not be recognized as different from named species based on readily observable traits. In this study, reference strains of different Aspergillus species, Penicillium chrysogenum, Candida albicans, and Cryptococcus neoformans were characterized using LC/MS and GC/MS biochemical profiling techniques in order to find specific small molecules, peptides or biochemical profiles that can be used in addition to established methods to detect and classify Aspergilli to the species level. Subsequently, analytical methods developed for characterizing the reference strains were applied, along with morphology and PCR, to characterize and identify several laboratory and field isolates. Some unique compounds and biochemical patterns did emerge from small molecule profiling that could be used for classifying Aspergilli, but protein profiling by LC/MS/MS was a much more effective approach. Tandem mass spectra from LC/MS/MS of tryptic peptides from fungal proteins were searched against protein databases and matched to theoretical spectra derived from those databases. Many of the amino acid sequences detected were taxonomically diagnostic for classifying Aspergillus species. Protein profiling also provided a great deal of additional biochemical information on the test organisms by identifying the predominant enzymes and structural proteins present under different experimental conditions and may find broader application for identifying and studying other organisms

A TARGETED LOAD-CARRIAGE TRAINING PROGRAM ELICITS POSITIVE ADAPTATIONS AFTER 10-WEEKS

The purpose of this study was to identify and characterise physical performance responses to a targeted 10-week load-carriage physical training intervention in males. Performance measures of maximal strength, heart rate, rating of perceived exertion, and basic fitness from nine male civilians before and after the 10-week training intervention are presented. There were significant increases in maximal force (~200 N) and aerobic performance (Level. Shuttle 8.9 vs 9,4 variables). Small-to-large effect sizes were shown for basic fitness and perceptual responses. The 10-week load-carriage physical training intervention elicited physical performance improvements and may facilitate load-carriage task performance

Life cycle assessment and feasibility study of small wind power in Thailand

The Thai government’s implementation of its 10 year renewable energy plan was done to help increase energy independence and reduce emissions resulting from energy production. Due to Thailand’s wind regime, wind turbines which can operate in low wind speeds will be needed to meet this goal. Small wind turbines typically operate at higher efficiency in lower winds, and thus they might prove to be a good option for wind power production in Thailand. Incorporating small wind turbines into power production can be difficult because of the perception of high investment costs and because their net benefit has not been adequately studied. Using a functional unit of producing 50 kWh per month for 10 years we conducted a Life Cycle Assessment comparing the global warming potential (GWP), embodied energy (EE), and levelized cost of electricity (LCOE) of four small wind turbines (≤20 kW), a diesel generator, and the Thai Grid. When analyzing GWP of the turbines it was found that they had a lower overall GWP than the diesel generator when in areas with reasonable wind resources. The same is true for embodied energy. Interestingly, in most available wind speed categories in Thailand the LCOE for wind turbines was lower than for the diesel generator. However, neither could compare to the LCOE of the Thai Grid, except in the areas with the highest average wind speeds (7.0 -­‐9.4 m/s). With this in mind, it is clear that the most important factor when considering wind power generation is the wind regime available in an area. Because of the increased cost relative to the Thai grid, implementation of wind turbines in Thailand was not found to be economically viable. This could be changed given lower costs for turbines and/or government incentives

Precision of the Integrated Cognitive Assessment for the assessment of neurocognitive performance in athletes at risk of concussion

Choice reaction time tests are commonly used for the assessment of cognitive function, and may be useful to assess the effect of mild traumatic injuries or concussions. This study investigated the precision of the Integrated Cognitive Assessment (ICA; Cognetivity Neurosciences Ltd., Vancouver, Canada) test for the assessment of cognitive function in athletes. Thirty-one participants volunteered to take part in this study, from both contact (n = 22) and non-contact sports (n = 9). Participants performed the ICA test consecutively both before and after normal training session to simulate resting and post-sport conditions. Precision errors were calculated for three variables, ICA Index (overall information processing ability), ICA Speed (information processing speed) and ICA Accuracy (information processing accuracy). ICA precision errors [root mean squared-standard deviation, RMS-SD (coefficient of variation, %CV)] pre-sport were ICA Index: 5.18 (7.14%), ICA Speed: 3.98 (4.64%), and ICA Accuracy: 3.64 (5.00%); and post-sport were ICA Index: 3.96 (4.94%), ICA Speed: 2.14 (2.32%), and ICA Accuracy 3.40 (4.25%). The ICA test demonstrates high in-vivo precision with all variables, with all variables except ICA Index (7.14%) demonstrating an acceptable precision error of ≤5% %CV. The ICA test is suitable for the assessment of cognitive function pre- and post-sport

THE SYMMETRY ANGLE IDENTIFIES LESS CLINICALLY RELEVANT INTER-LIMB ASYMMETRIES THAN THE SYMMETRY INDEX IN HEALTHY ADULTS

There are several methods for calculating inter-limb symmetry, an inter-limb difference ≥15% has been suggested as an indicator of sporting injury risk. The purpose of this study was to compare three common methods for determining symmetry: the Symmetry Index (percentage difference; SI) when referenced to the left limb (SILeft) or the average of both limbs (SIAverage), and the Symmetry Angle (vector difference; SA). 15 recreationally active participants completed a sprint protocol on a non-motorised treadmill. Accelerometers were positioned on both tibias to measure peak resultant acceleration (PRA). The SA identified less clinically relevant PRA inter-limb asymmetries than the SI in healthy adults. Once an appropriate level of asymmetry as measured by the SA is determined, this may help to more correctly identify asymmetry in athletes and patients than the SI

Measurement of lower-limb asymmetry in professional rugby league: a technical note describing the use of inertial measurement units

Background. Quantifying lower-limb load and asymmetry during team sport match play may be important for injury prevention and understanding performance. However, current analysis methods of lower-limb symmetry during match-play employ wearable microtechnology that may not be best suited to the task. A popular microtechnology is global positioning systems (GPS), which are torso worn. The torso location, and the summary workload measures calculated by GPS are not suited to the calculation of lower-limb load. Instead, research grade accelerometers placed directly on the lower-limb may provide better load information than GPS. This study proposes a new technique to quantify external mechanical load, and lower-limb asymmetry during on-field team sport play using inertial measurement units. Methods. Four professional rugby league players (Age: 23.4 ± 3.1 years; Height: 1.89 ± 0.05 m; Mass: 107.0 ± 12.9 kg) wore two accelerometers, one attached to each foot by the boot laces, during match simulations. Custom Matlab (R2017b, The Mathworks Inc, Natick, MA) code was used to calculate total time, area under the curve (AUC), and percentage of time (%Time) spent in seven acceleration categories (negative to very high, <0 g to >16 g), as well as minimum and maximum acceleration during match simulations. Lower-limb AUC and %Time asymmetry was calculated using the Symmetry Angle Equation, which does not require normalization to a reference leg. Results. The range of accelerations experienced across all participants on the left and right sides were 15.68–17.53 g, and 16.18–17.69 g, respectively. Clinically significant asymmetry in AUC and %Time was observed for all but one participant, and only in negative (<0 g) and very high accelerations (>16 g). Clinically significant AUC differences in very high accelerations ranged from 19.10%–26.71%. Clinically significant %Time differences in negative accelerations ranged from 12.65%–25.14%, and in very high accelerations from 18.59%–25.30%. All participants experienced the most AUC at very low accelerations (2–4 g), and the least AUC at very high accelerations (165.00–194.00 AU vs. 0.32–3.59 AU). The %Time results indicated that all participants spent the majority of match-play (73.82–92.06%) in extremely low (0–2 g) to low (4–6 g) acceleration intensities, and the least %Time in very high accelerations (0.01%–0.05%). Discussion. A wearable located on the footwear to measure lower-limb load and asymmetry is feasible to use during rugby league match-play. The location of the sensor on the boot is suited to minimize injury risk occurring from impact to the sensor. This technique is able to quantify external mechanical load and detect inter limb asymmetries during match-play at the source of impact and loading, and is therefore likely to be better than current torso based methods. The results of this study may assist in preparing athletes for match-play, and in preventing injury

It's OK to talk about suicide

Available from Devon County Council via the links in this recordDevon County Council factsheet. How to support someone who may be feeling suicidal. We are a group of people with personal or working experience of those who have either had suicidal thoughts or feelings or have lost someone to suicide. We want to share our knowledge and experience, so you are not alone when you are worried about someone who you think may be feeling suicidal. Suicidal thoughts are common but not permanent and can go away. Anyone can have suicidal thoughts, at any time in their life, whatever their background or situation. Suicide has many causes that are unique to each person and we must always take them seriously. To request a hard copy of this resource please email [email protected]. This information has been developed by Devon County Council (N Glassbrook) in partnership with Exeter University (P Xanthopoulou), Devon Partnership NHS Trust (G Adams and D Frances), Parental Minds (S Glover) and people with lived experience

The Demands of Professional Rugby League Match-Play: a Meta-analysis

Background Rugby league is a collision sport, where players are expected to be physically competent in a range of areas, including aerobic fitness, strength, speed and power. Several studies have attempted to characterise the physical demands of rugby league match-play, but these studies often have relatively small sample sizes based on one or two clubs, which makes generalisation of the findings difficult. Therefore, the aim of this review was to synthesise studies that investigated the physical demands of professional rugby league match-play. Methods SPORTDiscus, CINAHL, MEDLINE (EBSCO) and Embase (EBSCO) databases were systematically searched from inception until October 2018. Articles were included if they (1) recruited professional rugby league athletes aged ≥ 18 years and (2) provided at least one match-play relevant variable (including playing time, total and relative distance, repeat high-intensity efforts (RHIE), efforts per RHIE, accelerations and decelerations, total and relative collisions). Meta-analyses were used to provide pooled estimates ± 95% confidence intervals. Results A total of 30 studies were included. Pooled estimates indicated that, compared to adjustables and backs, forwards have less playing time (− 17.2 ± 5.6 and − 25.6 ± 5.8 min, respectively), cover less ‘slow-speed’ (− 2230 ± 735 and − 1348 ± 655 m, respectively) and ‘high-speed’ distance (− 139 ± 108 and − 229 ± 101 m, respectively), but complete more relative RHIEs (+ 0.05 ± 0.05 and + 0.08 ± 0.04 per minute, respectively), and total (+ 12.0 ± 8.1 and + 12.8 ± 7.2 collisions, respectively) and relative collisions (+ 0.32 ± 0.22 and + 0.41 ± 0.22 collisions per minute, respectively). Notably, when the distance was expressed relative to playing time, forwards were not different from adjustables and backs in slow-speed (P ≥ 0.295) and high-speed (P ≥ 0.889) relative distance. The adjustables and backs subgroups were similar in most variables, except playing time (shorter for adjustables, − 8.5 ± 6.2 min), slow-speed distance (greater for adjustables, + 882 ± 763 m) and total relative distance (greater for adjustables, + 11.3 ± 5.2 m·min−1). There were no significant differences between positional groups for efforts per RHIE, accelerations and decelerations (P ≥ 0.745). Conclusions These results indicate the unique physical demands of each playing position and should be considered by strength and conditioning and tactical coaches when planning for professional rugby league performance

The Challenge of Applying and Undertaking Research in Female Sport.

In recent years there has been an exponential rise in the professionalism and success of female sports. Practitioners (e.g., sport science professionals) aim to apply evidence-informed approaches to optimise athlete performance and well-being. Evidence-informed practices should be derived from research literature. Given the lack of research on elite female athletes, this is challenging at present. This limits the ability to adopt an evidence-informed approach when working in female sports, and as such, we are likely failing to maximize the performance potential of female athletes. This article discusses the challenges of applying an evidence base derived from male athletes to female athletes. A conceptual framework is presented, which depicts the need to question the current (male) evidence base due to the differences of the "female athlete" and the "female sporting environment," which pose a number of challenges for practitioners working in the field. Until a comparable applied sport science research evidence base is established in female athletes, evidence-informed approaches will remain a challenge for those working in female sport