The ‘Evergrowing’ genotype of Corylus avellana is expressed in the offspring of ‘Tonda Gentile Romana’, ‘Nocchione’ and ‘Tonda di Giffoni’

The hazelnut (Corylus avellana L.) ‘evergrowing’ phenotype (EVG) fails both to cease growth and to enter dormancy under the dormancy-inducing (i.e., short days) conditions suitable for the wild type. The EVG phenotype is expressed by the homozygous genotype for the recessive allele EVG-d.L'articolo é disponibile sul sito dell'editore: http://www.ishs.or

Morphological, Molecular, and Nutritional Characterisation of the Globe Artichoke Landrace “Carciofo Ortano”

The present study focused on the molecular, morphological, and nutritional characterisation of a globe artichoke landrace at risk of genetic erosion still cultivated in the municipality of Orte (Lazio Region, Central Italy) and therefore named “Carciofo Ortano”. Molecular analysis based on SSR and ISSR markers was carried out on 73 genotypes selected at random from 20 smallholdings located in the Orte countryside and 17 accessions of landraces/clones belonging to the main varietal types cultivated in Italy. The results confirmed that “Carciofo Ortano” belongs to the “Romanesco” varietal typology and revealed the presence within the landrace of two distinct genetic populations named Orte 1 and Orte 2. Despite the high level of within-population genetic variation detected, the two populations were genetically differentiated from each other and from the landraces/clones of the main varietal types cultivated in Italy. Morphological and nutritional characterisation was performed on representative genotypes for each of the two populations of the “Carciofo Ortano” and the four landraces/clones included in the varietal platform of the PGI “CARCIOFO ROMANESCO DEL LAZIO” used as reference genotypes (“Campagnano”, “Castellammare”, “C3”, and “Grato 1”). Principal component analysis showed that, of the 43 morphological descriptors considered, 12, including plant height, head shape index, head yield, and earliness, allowed a clear grouping of genotypes, distinguishing Orte 1 and Orte 2 populations from the reference genotypes. Regarding the nutritional composition of heads, particular attention should be devoted to the Orte 2 genotypes for their high dietary fibre, inulin, flavonoid, and phenol content, a feature that could be highly appreciated by the market

Jasmonate-mediated defence responses, unlike salicylate-mediated responses, are involved in the recovery of grapevine from bois noir disease

Abstract Background Bois noir is an important disease of grapevine (Vitis vinifera L.), caused by phytoplasmas. An interesting, yet elusive aspect of the bois noir disease is “recovery”, i.e., the spontaneous and unpredictable remission of symptoms and damage. Because conventional pest management is ineffective against bois noir, deciphering the molecular bases of recovery is beneficial. The present study aimed to understand whether salicylate- and jasmonate-defence pathways might have a role in the recovery from the bois noir disease of grapevine. Results Leaves from healthy, bois noir-diseased and bois noir-recovered plants were compared, both in the presence (late summer) and absence (late spring) of bois noir symptoms on the diseased plants. Analyses of salicylate and jasmonate contents, as well as the expression of genes involved in their biosynthesis, signalling and action, were evaluated. In symptomatic diseased plants (late summer), unlike symptomless plants (late spring), salicylate biosynthesis was increased and salicylate-responsive genes were activated. In contrast, jasmonate biosynthesis and signalling genes were up-regulated both in recovered and diseased plants at all sampling dates. The activation of salicylate signalling in symptomatic plants might have antagonised the jasmonate-mediated defence response by suppressing the expression of jasmonate-responsive genes. Conclusions Our results suggest that grapevine reacts to phytoplasma infection through salicylate-mediated signalling, although the resultant full activation of a salicylate-mediated response is apparently ineffective in conferring resistance against bois noir disease. Activation of the salicylate signalling pathway that is associated with the presence of bois noir phytoplasma seems to antagonise the jasmonate defence response, by failing to activate or suppressing both the expression of some jasmonate responsive genes that act downstream of the jasmonate biosynthetic pathway, as well as the first events of the jasmonate signalling pathway. On the other hand, activation of the entire jasmonate signalling pathway in recovered plants suggests the potential importance of jasmonate-regulated defences in preventing bois noir phytoplasma infections and the subsequent development of bois noir disease. Thus, on one hand, recovery could be achieved and maintained over time by preventing the activation of defence genes associated with salicylate signalling, and on the other hand, by activating jasmonate signalling and other defence responses

Genetic Diversity and Population Structure of Common Bean (<i>Phaseolus vulgaris</i> L.) Landraces in the Lazio Region of Italy

Common bean cultivation has historically been a typical component of rural economies in Italy, particularly in mountainous and hilly zones along the Apennine ridge of the central and southern regions, where the production is focused on local landraces cultivated by small-scale farmers using low-input production systems. Such landraces are at risk of genetic erosion because of the recent socioeconomic changes in rural communities. One hundred fourteen accessions belonging to 66 landraces still being grown in the Lazio region were characterized using a multidisciplinary approach. This approach included morphological (seed traits), biochemical (phaseolin and phytohemagglutinin patterns), and molecular (microsatellite loci) analyses to investigate their genetic variation, structure, and distinctiveness, which will be essential for the implementation of adequate ex situ and in situ conservation strategies. Another objective of this study was to determine the original gene pool (Andean and Mesoamerican) of the investigated landraces and to evaluate the cross-hybridization events between the two ancestral gene pools in the P. vulgaris germplasm in the Lazio region. Molecular analyses on 456 samples (four for each of the 114 accessions) revealed that the P. vulgaris germplasm in the Lazio region exhibited a high level of genetic diversity (He = 0.622) and that the Mesoamerican and Andean gene pools were clearly differentiated, with the Andean gene pool prevailing (77%) and 12% of landraces representing putative hybrids between the two gene pools. A model-based cluster analysis based on the molecular markers highlighted three main groups in agreement with the phaseolin patterns and growth habit of landraces. The combined utilisation of morphological, biochemical, and molecular data allowed for the differentiation of all landraces and the resolution of certain instances of homonymy and synonymy. Furthermore, although a high level of homozygosity was found across all landraces, 32 of the 66 examined (49%) exhibited genetic variability, indicating that the analysis based on a single or few plants per landrace, as usually carried out, may provide incomplete information

Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces in the Lazio Region of Italy

Common bean cultivation has historically been a typical component of rural economies in Italy, particularly in mountainous and hilly zones along the Apennine ridge of the central and southern regions, where the production is focused on local landraces cultivated by small-scale farmers using low-input production systems. Such landraces are at risk of genetic erosion because of the recent socioeconomic changes in rural communities. One hundred fourteen accessions belonging to 66 landraces still being grown in the Lazio region were characterized using a multidisciplinary approach. This approach included morphological (seed traits), biochemical (phaseolin and phytohemagglutinin patterns), and molecular (microsatellite loci) analyses to investigate their genetic variation, structure, and distinctiveness, which will be essential for the implementation of adequate ex situ and in situ conservation strategies. Another objective of this study was to determine the original gene pool (Andean and Mesoamerican) of the investigated landraces and to evaluate the cross-hybridization events between the two ancestral gene pools in the P. vulgaris germplasm in the Lazio region. Molecular analyses on 456 samples (four for each of the 114 accessions) revealed that the P. vulgaris germplasm in the Lazio region exhibited a high level of genetic diversity (He = 0.622) and that the Mesoamerican and Andean gene pools were clearly differentiated, with the Andean gene pool prevailing (77%) and 12% of landraces representing putative hybrids between the two gene pools. A model-based cluster analysis based on the molecular markers highlighted three main groups in agreement with the phaseolin patterns and growth habit of landraces. The combined utilisation of morphological, biochemical, and molecular data allowed for the differentiation of all landraces and the resolution of certain instances of homonymy and synonymy. Furthermore, although a high level of homozygosity was found across all landraces, 32 of the 66 examined (49%) exhibited genetic variability, indicating that the analysis based on a single or few plants per landrace, as usually carried out, may provide incomplete information

Additional file 1: Table S1. of Jasmonate-mediated defence responses, unlike salicylate-mediated responses, are involved in the recovery of grapevine from bois noir disease

Vitis vinifera gene sequences coding for phenylalanine ammonia-lyase (PAL) and isochorismate synthase (ICS), identified in the National Center for Biotechnology Information (NCBI) database and used in the synthesis of oligonucleotides for expression analysis. Table S2. Vitis vinifera gene sequences coding for lipoxygenase (LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC), 12-oxo-phytodienoic acid (OPDA) reductase (OPR), and jasmonate carboxyl methyltransferase (JMT), identified in the NCBI database and used in the synthesis of oligonucleotides for expression analysis. Table S3. Vitis vinifera gene sequences involved in salicylate (NPR1.1, NPR1.2 and EDS1) or jasmonate (MYC2, JAZ1, JAZ2 and JAZ3) signalling pathways, identified in the NCBI database and used in the synthesis of oligonucleotides for expression analysis. Table S4. Vitis vinifera gene sequences coding for WRKY transcription factors, identified in the NCBI database and used in the synthesis of oligonucleotides for expression analysis. Table S5. Vitis vinifera gene sequences coding for pathogenesis-related proteins (PRP), identified in the NCBI database and used in the synthesis of oligonucleotides for expression analysis. Table S6. Vitis vinifera gene sequences coding for stilbene synthase (STS) and MYB transcription factors that specifically interact with the STS promoter, identified in the NCBI database and used in the synthesis of oligonucleotides for expression analysis. Table S7. Vitis vinifera gene sequences coding for chalcone synthase (CHS), identified in the NCBI database and used in the synthesis of oligonucleotides for expression analysis. Table S8. Vitis vinifera sequences coding for candidate reference genes, identified in the NCBI database and used in the synthesis of oligonucleotides for the normalisation of expression data in qRT-PCR analyses. (DOC 358 kb