'I think you understand me' : studying the associations between actual, assumed, and perceived understanding within couples

The current study examined the associations between actual, assumed, and perceived understanding and partners' levels of dyadic adjustment. One hundred fifty-two couples provided questionnaire data (assumed and perceived understanding), participated in a videotaped conflict interaction, and in a video-review task to assess actual understanding (empathic accuracy). The data were analyzed by means of the Actor-Partner Interdependence Model. The results suggest that (a) some aspects of how well someone assumes that (s)he has understood the partner during a preceding conflict interaction were positively associated with his/her own objective level of understanding (actor effect), (b) that someone's perception of how understood (s)he feels was not associated with the partner's objective level of understanding (partner effect), and (c) perceived understanding, but not actual understanding, was positively associated with dyadic adjustment

Multilevel autoregressive models when the number of time points is small

The multilevel autoregressive model disentangles unobserved heterogeneity from state-dependence. Statistically, the random intercept accounts for the dependence of all measurements at different time points on an observed underlying factor, while the lagged dependent predictor allows the outcome to depend on the outcome at the previous time point. In this paper, we consider different implementations of the simplest multilevel autoregressive model, and explore how each of them deals with the endogeneity assumption and the initial conditions problem. We discuss the performance of the no centering approach, the manifest centering approach, and the latent centering approach in the setting where the number of time points is small. We find that some commonly used approaches show bias for the autoregressive parameter. When the outcome at the first time point is considered predetermined, the no centering approach assuming endogeneity performs best

Multilevel autoregressive models for longitudinal dyadic data

In social and behavioral science, dyadic research has become more and more popular. In case of cross-sectional dyadic data, one can apply the actor-partner interdependence model (APIM). When dyads are measured repeatedly over time, applied researchers are often hesitant to analyze such data due to the statistical complexity. In this paper, we introduce a user-friendly Shiny-application, called the LDDinSEM-application. The app automatically fits the lagged dependent actor-partner interdependence model (LD-APIM), a multilevel autoregressive model extension of the APIM within the structural equation modeling (SEM) framework. The application allows the researcher to investigate the effects of an antecedent on an outcome, given the previous outcome. We illustrate the app using an empirical example assessing the actor and partner effects of positive relationship feelings on next day's intimacy in heterosexual couples

Drosophila Models of Tauopathies: What Have We Learned?

Aggregates of the microtubule-associated protein Tau are neuropathological hallmark lesions in Alzheimer's disease (AD) and related primary tauopathies. In addition, Tau is genetically implicated in a number of human neurodegenerative disorders including frontotemporal dementia (FTD) and Parkinson's disease (PD). The exact mechanism by which Tau exerts its neurotoxicity is incompletely understood. Here, we give an overview of how studies using the genetic model organism Drosophila over the past decade have contributed to the molecular understanding of Tau neurotoxicity. We compare the different available readouts for Tau neurotoxicity in flies and review the molecular pathways in which Tau has been implicated. Finally, we emphasize that the integration of genome-wide approaches in human or mice with high-throughput genetic validation in Drosophila is a fruitful approach

New insights on the clinical variability of FKBP10 mutations

To date 45 autosomal recessive disease-causing variants are reported in the FKBP10 gene. Those variant were found to be associated with Osteogenesis Imperfecta (OI) for which the hallmark phenotype is bone fractuers or Bruck Syndrome (BS) where bone fractures are accompanied with contractures. In addition, a specific homozygous FKBP10 mutation (p.Tyr293del) has been described in Yup'ik Inuit population to cause Kuskokwim syndrome (KS) in which contractures without fractures are observed. Here we present an extended Palestinian family with 10 affected individuals harboring a novel homozygous splice site mutation, c.391+4A > T in intron 2 of the FKBP10 gene, in which the three above mentioned syndromes segregate as a result of skipping of exon 2 and absence of the FKBP65 protein. At the biochemical level, Hydroxylysyl pyridinoline (HP)/lysyl pyridinoline (LP) values were inversely correlated with OI phenotypes, a trend we could confirm in our patients. Our findings illustrate that single familial FKBP10 mutations can result in a phenotypic spectrum, ranging from fractures without contractures, to fractures and contractures and even to only contractures. This broad intrafamilial clinical variability within one single family is a new finding in the field of bone fragility

Developmental expression of 4-repeat-Tau induces neuronal aneuploidy in Drosophila tauopathy models

Tau-mediated neurodegeneration in Alzheimer's disease and tauopathies is generally assumed to start in a normally developed brain. However, several lines of evidence suggest that impaired Tau isoform expression during development could affect mitosis and ploidy in post-mitotic differentiated tissue. Interestingly, the relative expression levels of Tau isoforms containing either 3 (3R-Tau) or 4 repeats (4R-Tau) play an important role both during brain development and neurodegeneration. Here, we used genetic and cellular tools to study the link between 3R and 4R-Tau isoform expression, mitotic progression in neuronal progenitors and post-mitotic neuronal survival. Our results illustrated that the severity of Tau-induced adult phenotypes depends on 4R-Tau isoform expression during development. As recently described, we observed a mitotic delay in 4R-Tau expressing cells of larval eye discs and brains. Live imaging revealed that the spindle undergoes a cycle of collapse and recovery before proceeding to anaphase. Furthermore, we found a high level of aneuploidy in post-mitotic differentiated tissue. Finally, we showed that overexpression of wild type and mutant 4R-Tau isoform in neuroblastoma SH-SY5Y cell lines is sufficient to induce monopolar spindles. Taken together, our results suggested that neurodegeneration could be in part linked to neuronal aneuploidy caused by 4R-Tau expression during brain development