Kinetics and Mechanism of Reduction of 4(p-Dimethylamino)-Benzylidene-3-Methyl-1-Phenyl-2-Pyrazolin-5-one by Bisulphite Ions in Aqueous Ethanolic Media

The kinetics of the title reduction studied in aqueous ethanolic media spectrophotometrically. The effect of hydrogen ion concentration on the reduction rate was investigated in buffer solutions at constant ionic strength of 1.0. The reaction was found to be of the first order in both reactants and inverse first order with respect to the hydrogen ion. The rate of the reaction decreased with increasing ethanol as well as hydrogen ion concentrations. Pseudo-first-order rate constant, kob was obtained by monitoring the disappearance of the [dye]. Activation parameters have been evaluated and a tentative reaction mechanism was discussed

Automatic Software Test Data Generation for Spanning Sets Coverage Using Genetic Algorithms

Software testing takes a considerable amount of time and resources spent on producing software. Therefore, it would be useful to have ways to reduce the cost of software testing. The new concepts of spanning sets of entities suggested by Marré and Bertolino are useful for reducing the cost of testing. In fact, to reduce the testing effort, the generation of test data can be targeted to cover the entities in the spanning set, rather than all the entities in the tested program. Marré and Bertolino presented an algorithm based on the subsumption relation between entities to find spanning sets for a family of control flow and data flow-based test coverage criteria. This paper presents a new general technique for the automatic test data generation for spanning sets coverage. The proposed technique applies to the algorithm proposed recently by Marré and Bertolino to automatically generate the spanning sets of program entities that satisfy a wide range of control flow and data flow-based test coverage criteria. Then, it uses a genetic algorithm to automatically generate sets of test data to cover these spanning sets. The proposed technique employed the concepts of spanning sets to limit the number of test cases, guide the test case selection, overcome the problem of the redundant test cases and automate the test path generation

Response of maize inbred lines to two European corn borer (Ostrinia nubilalis) strains in Canada

Six lignées de maïs-grain (CM47, A619, F2, CM107, CM7 et A654) ont été évaluées en 1986 et 1987 dans quatre localités de l'Ontario et du Québec pour leur résistance à des infestations artificielles de la pyrale du maïs, Ostrinia nubilalis, de la race univoltine et de la première génération de la race bivoltine. Trois critères ont été utilisés: la criblure du feuillage, les dégâts totaux des plantes à la récolte, et le rapport longueur des galeries creusées par les chenilles dans les tiges sur la hauteur des plantes. Des interactions importantes ont été observées pour les critères utilisés entre les années et localités, mais la réaction des lignées de maïs a été plutôt constante. Pour tous les critères, la race univoltine a souvent causé, d'une façon significative, plus de dégâts aux plantes que la race bivoltine. En général, la lignée A619 a démontré le plus de résistance-tolérance tout en conservant une bonne tenue des tiges jusqu'à la récolte. On aurait avantage à utiliser la race univoltine dans les programmes d'amélioration génétique du maïs pour sa résistance à la pyrale.In 1986 and 1987, six maize inbred lines (CM47, A619, F2, CM107, CM7, and A654) were evaluated at four locations across Ontario and Quebec for their resistance to artificially infested univoltine and lst generation bivoltine strains of the European corn borer, Ostrinia nubilalis. Three criteria were used: leaf feeding, total plant damage at harvest and length of tunnels/plant height ratio. Substantial interactions in borer damage measurements were observed between locations and years, but inbred reaction was relatively consistent. For all criteria, the univoltine strain often caused significantly more damage than the bivoltine borer. In general, A619 had the greatest resistance-tolerance with good standability until harvest. Wherever possible, evaluation of genetic resistance in maize germplasm should be conducted using the univoltine borer strain

Application of the group-theoretical method to physical problems

The concept of the theory of continuous groups of transformations has attracted the attention of applied mathematicians and engineers to solve many physical problems in the engineering sciences. Three applications are presented in this paper. The first one is the problem of time-dependent vertical temperature distribution in a stagnant lake. Two cases have been considered for the forms of the water parameters, namely water density and thermal conductivity. The second application is the unsteady free-convective boundary-layer flow on a non-isothermal vertical flat plate. The third application is the study of the dispersion of gaseous pollutants in the presence of a temperature inversion. The results are found in closed form and the effect of parameters are discussed

Indicators of physical stress during hindlimb suspension in Wistar rats / Indicadores de stress físico durante a suspensão dos membros inferiores em ratos Wistar

Hindlimb suspension (HS) has been used in animal research as a microgravity simulation model. However, physical stress to the animals during suspension has received little attention. The purpose of this work was to investigate physical stress, oxidative stress, and cross-sectional area (CSA) in rats submitted to hindlimb suspension. Twelve male Wistar rats were allocated into two groups: control (CON, n = 6); hindlimb suspension (HS, n = 6), for ten days. The following signs of physical stress were monitored: bristly hair, spleen hair, edema in the nose, edema or necrosis in the tail, edema in the hind legs, aggressiveness, redness in the dorsal region, body weight loss, and food intake. Samples of the soleus and tibialis anterior were prepared for CSA and carbonylated proteins analysis. The frequency of the signs of stress, except redness in the dorsal region, was significantly higher (P <.05) in the HS group. CSA of soleus (816.6µm2, 95%CI: 866.3-904.0 vs 1158.8mm2, 95%CI: 1171.9-1214.2) and tibialis anterior (1041.5µm2, 95%CI: 1119.1-1185.2 vs 1429.8µm2, 95%CI: 1479.2-1566.4) were lower in the HS group (P <.05). Carbonylated proteins in the soleus (2779.0 ± 1319.8 AU vs 1063.2 ± 289.2 AU) and tibialis anterior (1155.8 AU, 95%CI: 80.0-4064.5 vs 108.4 AU, 95%CI: 35.5-320.7) were higher in the HS group (P <.05). The suspension model evokes oxidative stress and reduced CSA. However, several signs of physical stress can occur. This indicates that hindlimb suspension models may introduce confounding factors that influence muscle atrophy and should be used cautiously.

Articular contact in a three-dimensional model of the knee

This study is aimed at the analysis of articular contact in a three-dimensional mathematical model of the human knee-joint. In particular the effect of articular contact on the passive motion characteristics is assessed in relation to experimentally obtained joint kinematics. Two basically different mathematical contact descriptions were compared for this purpose. One description was for rigid contact and one for deformable contact. The description of deformable contact is based on a simplified theory for contact of a thin elastic layer on a rigid foundation. The articular cartilage was described either as a linear elastic material or as a non-linear elastic material. The contact descriptions were introduced in a mathematical model of the knee. The locations of the ligament insertions and the geometry of the articular surfaces were obtained from a joint specimen of which experimentally determined kinematic data were available, and were used as input for the model. The ligaments were described by non-linear elastic line elements. The mechanical properties of the ligaments and the articular cartilage were derived from literature data. Parametric model evaluations showed that, relative to rigid articular contact, the incorporation of deformable contact did not alter the motion characteristics in a qualitative sense, and that the quantitative changes were small. Variation of the elasticity of the elastic layer revealed that decreasing the surface stiffness caused the ligaments to relax and, as a consequence, increased the joint laxity, particularly for axial rotation. The difference between the linear and the non-linear deformable contact in the knee model was very small for moderate loading conditions. The motion characteristics simulated with the knee model compared very well with the experiments. It is concluded that for simulation of the passive motion characteristics of the knee, the simplified description for contact of a thin linear elastic layer on a rigid foundation is a valid approach when aiming at the study of the motion characteristics for moderate loading conditions. With deformable contact in the knee model, geometric conformity between the surfaces can be modelled as opposed to rigid contact which assumed only point contact

Disparities in the Uptake of Telemedicine During the COVID-19 Surge in a Multidisciplinary Head and Neck Cancer Population by Patient Demographic Characteristics and Socioeconomic Status

This cohort study examines the association between patient demographic characteristics and socioeconomic status and engagement in telemedicine during the COVID-19 pandemic

1,25-Dihydroxycholecalciferol (calcitriol) modifies uptake and release of 25-hydroxycholecalciferol in skeletal muscle cells in culture

© 2017 Elsevier Ltd The major circulating metabolite of vitamin D 3 , 25-hydroxycholecalciferol [25(OH)D], has a remarkably long half-life in blood for a (seco)steroid. Data from our studies and others are consistent with the hypothesis that there is a role for skeletal muscle in the maintenance of vitamin D status. Muscle cells internalise vitamin D-binding protein (DBP) from the circulation by means of a megalin/cubilin plasma membrane transport mechanism. The internalised DBP molecules then bind to actin and thus provide an intracellular array of high affinity binding sites for its specific ligand, 25(OH)D. There is evidence that the residence time for DBP in muscle cells is short and that it undergoes proteolytic degradation, releasing bound 25(OH)D. The processes of internalisation of DBP and its intracellular residence time, bound to actin, appear to be regulated. To explore whether 1,25-dihydroxycholecalciferol (calcitriol) has any effect on this process, cell cultures of myotubes and primary skeletal muscle fibers were incubated in a medium containing 10 −10 M calcitriol but with no added DBP. After 3 h pre-incubation with calcitriol, the net uptake of 25(OH)D by these calcitriol-treated cells over a further 4 h was significantly greater than that in vehicle-treated control cells. This was accompanied by a significant increase in intracellular DBP protein. However, after 16 h of pre-incubation with calcitriol, the muscle cells showed a significantly depressed ability to accumulate 25(OH)D compared to control cells over a further 4 or 16 hours. These effects of pre-incubation with calcitriol were abolished in fibers from VDR-knockout mice. The effect was also abolished by the addition of 4,4\u27-diisothiocyano-2,2\u27-stilbenedisulfonic acid (DIDS), which inhibits chloride channel opening. Incubation of C2 myotubes with calcitriol also significantly reduced retention of previously accumulated 25(OH)D after 4 or 8 h. It is concluded from these in vitro studies that calcitriol can modify the DBP-dependent uptake and release of 25(OH)D by skeletal muscle cells in a manner that suggests some inducible change in the function of these cells