2 research outputs found
Additional file 1: of The immune suppressive microenvironment of human gliomas depends on the accumulation of bone marrow-derived macrophages in the center of the lesion
Table S1. Cell populations analyzed in tumor tissues of glioma patients. Supplementary materials and methods. Description of patient characteristics, multiparametric flow cytometry, functional assay, t-SNE analysis and experiments with nanoparticles. (DOCX 22 kb
CO<sub>2</sub>‑Driven Nebulization of pH-Sensitive Supramolecular Polymers for Intraperitoneal Hydrogel Formation and the Treatment of Peritoneal Metastasis
Because peritoneal metastasis (PM) from ovarian cancer
is characterized
by non-specific symptoms, it is often diagnosed at advanced stages.
Pressurized intraperitoneal aerosol chemotherapy (PIPAC) can be considered
a promising drug delivery method for unresectable PM. Currently, the
efficacy of intraperitoneal (IP) drug delivery is limited by the off-label
use of IV chemotherapeutic solutions, which are rapidly cleared from
the IP cavity. Hence, this research aimed to improve PM treatment
by evaluating a nanoparticle-loaded, pH-switchable supramolecular
polymer hydrogel as a controlled release drug delivery system that
can be IP nebulized. Moreover, a multidirectional nozzle was developed
to allow nebulization of viscous materials such as hydrogels and to
reach an even IP gel deposition. We demonstrated that acidification
of the nebulized hydrogelator solution by carbon dioxide, used to
inflate the IP cavity during laparoscopic surgery, stimulated the in situ gelation, which prolonged the IP hydrogel retention. In vitro experiments indicated that paclitaxel nanocrystals
were gradually released from the hydrogel depot formed, which sustained
the cytotoxicity of the formulation for 10 days. Finally, after aerosolization
of this material in a xenograft model of PM, tumor progression could
successfully be delayed, while the overall survival time was significantly
increased compared to non-treated animals