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Spatial restriction of alpha4 integrin phosphorylation regulates lamellipodial stability and alpha4beta1-dependent cell migration.
Integrins coordinate spatial signaling events essential for cell polarity and directed migration. Such signals from alpha4 integrins regulate cell migration in development and in leukocyte trafficking. Here, we report that efficient alpha4-mediated migration requires spatial control of alpha4 phosphorylation by protein kinase A, and hence localized inhibition of binding of the signaling adaptor, paxillin, to the integrin. In migrating cells, phosphorylated alpha4 accumulated along the leading edge. Blocking alpha4 phosphorylation by mutagenesis or by inhibition of protein kinase A drastically reduced alpha4-dependent migration and lamellipodial stability. alpha4 phosphorylation blocks paxillin binding in vitro; we now find that paxillin and phospho-alpha4 were in distinct clusters at the leading edge of migrating cells, whereas unphosphorylated alpha4 and paxillin colocalized along the lateral edges of those cells. Furthermore, enforced paxillin association with alpha4 inhibits migration and reduced lamellipodial stability. These results show that topographically specific integrin phosphorylation can control cell migration and polarization by spatial segregation of adaptor protein binding
Superconducting properties of ultrathin Bi2Sr2CaCu2O8+x single crystals
We use Ar-ion milling to thin Bi2212 single crystals down to a few nanometers
or one-to-two (CuO2)2 layers. With decreasing the thickness, superconducting
transition temperature gradually decreases to zero and the in-plane resistivity
increases to large values indicating the existence of a
superconductor-insulator transition in ultrathin Bi2212 single crystals.Comment: 17 pages, 6 figures, to appear in J. Appl. Phys. 98(3) 200
Non-Additive Interactions Unlock Small-Particle Mobility in Binary Colloidal Monolayers
We examine the organization and dynamics of binary colloidal monolayers
composed of micron-scale silica particles interspersed with smaller-diameter
silica particles that serve as minority component impurities. These binary
monolayers are prepared at the surface of ionic liquid droplets over a range of
size ratios () and are studied with low-dose minimally
perturbative scanning electron microscopy (SEM). The high resolution of SEM
imaging provides direct tracking of all particle coordinates over time,
enabling a complete description of the microscopic state. In these bidisperse
size mixtures, particle interactions are non-additive because interfacial
pinning to the droplet surface causes the equators of differently sized
particles to lie in separate planes. By varying the size ratio we control the
extent of non-additivity in order to achieve phase behavior inaccessible to
strictly 2D systems. Across the range of size ratios we tune the system from a
mobile small-particle phase (), to an interstitial solid
(). These distinct
phase regimes are classified through measurements of hexagonal ordering of the
large-particle host lattice and the lattice's capacity for small-particle
transport. Altogether, we explain these structural and dynamic trends by
considering the combined influence of interparticle interactions and the
colloidal packing geometry. Our measurements are reproduced in molecular
dynamics simulations of 2D non-additive hard disks, suggesting an efficient
method for describing confined systems with reduced dimensionality
representations.Comment: 12 pages, 7 figures, also see supplementary ancillary fil
Cathodoluminescence-based nanoscopic thermometry in a lanthanide-doped phosphor
Crucial to analyze phenomena as varied as plasmonic hot spots and the spread
of cancer in living tissue, nanoscale thermometry is challenging: probes are
usually larger than the sample under study, and contact techniques may alter
the sample temperature itself. Many photostable nanomaterials whose
luminescence is temperature-dependent, such as lanthanide-doped phosphors, have
been shown to be good non-contact thermometric sensors when optically excited.
Using such nanomaterials, in this work we accomplished the key milestone of
enabling far-field thermometry with a spatial resolution that is not
diffraction-limited at readout.
We explore thermal effects on the cathodoluminescence of lanthanide-doped
NaYF nanoparticles. Whereas cathodoluminescence from such lanthanide-doped
nanomaterials has been previously observed, here we use quantitative features
of such emission for the first time towards an application beyond localization.
We demonstrate a thermometry scheme that is based on cathodoluminescence
lifetime changes as a function of temperature that achieves 30 mK
sensitivity in sub-m nanoparticle patches. The scheme is robust against
spurious effects related to electron beam radiation damage and optical
alignment fluctuations.
We foresee the potential of single nanoparticles, of sheets of nanoparticles,
and also of thin films of lanthanide-doped NaYF to yield temperature
information via cathodoluminescence changes when in the vicinity of a sample of
interest; the phosphor may even protect the sample from direct contact to
damaging electron beam radiation. Cathodoluminescence-based thermometry is thus
a valuable novel tool towards temperature monitoring at the nanoscale, with
broad applications including heat dissipation in miniaturized electronics and
biological diagnostics.Comment: Main text: 30 pages + 4 figures; supplementary information: 22 pages
+ 8 figure
Correlating Pedestrian Flows and Search Engine Queries
An important challenge for ubiquitous computing is the development of
techniques that can characterize a location vis-a-vis the richness and
diversity of urban settings. In this paper we report our work on correlating
urban pedestrian flows with Google search queries. Using longitudinal data we
show pedestrian flows at particular locations can be correlated with the
frequency of Google search terms that are semantically relevant to those
locations. Our approach can identify relevant content, media, and
advertisements for particular locations.Comment: 4 pages, 1 figure, 1 tabl
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