Prolactin and Male Fertility: The Long and Short Feedback Regulation

In the last 20 years, a pituitary-hypothalamus tissue culture system with intact neural and portal connections has been developed in our lab and used to understand the feedback mechanisms that regulate the secretions of adenohypophyseal hormones and fertility of male rats. In the last decade, several in vivo rat models have also been developed in our lab with a view to substantiate the in vitro findings, in order to delineate the role of pituitary hormones in the regulation of fertility of male rats. These studies have relied on both surgical and pharmacological interventions to modulate the secretions of gonadotropins and testosterone. The interrelationship between the circadian release of reproductive hormones has also been ascertained in normal men. Our studies suggest that testosterone regulates the secretion of prolactin through a long feedback mechanism, which appears to have been conserved from rats to humans. These studies have filled in a major lacuna pertaining to the role of prolactin in male reproductive physiology by demonstrating the interdependence between testosterone and prolactin. Systemic levels of prolactin play a deterministic role in the mechanism of chromatin condensation during spermiogenesis

Modeling used for Software Product Line Engineering

Software product line is the separation of variant features of all the products which belong to same line. Modeling is the basic foundation of Software Product Line Engineering, that is used for collection of what is similar and what is different between products, but products of same line. Here Line means a set of products those are related and share some commonalities like data structures, software components, some features and architecture etc.In order to managing the variability and commonalties in product line we use modeling in Software product line.So that SPLE is the most powerful approach to which we can use for to increase the efficiency of the software engineering process and we can develop variety of software from a single software product line, that’s why if we implement low design that can ripple through many generated software systems.In this paper I represent the relationship between Orthogonal Variability model and various different qualities attributes affecting them., I will also describe some existing metrics which we use to measure these quality attributes

Efficacy of anthranilic insecticide E2Y45 20 SC (Chlorantraniliprole) against Plutella xylostella L. in cabbage, Brassica oleracea var. capitata

Experiments were conducted at farmer’s cabbage fields to evaluate the bioefficacy of anthranilic insecticide i.e. E2Y45 20 SC (Chlorantraniliprole) having novel mode of action against the diamondback moth Plutella xylostella L. E2Y45 20 SC was applied @ 25.0, 37.5 and 50.0 ml/ha and was compared with Padan 50 SP (cartap hydrochloride) @ 500 g/ha and the untreated control. Lowest mean larval population after two sprays was recorded in higher dose of E2Y 45 20 SC i.e. @ 50.0 ml/ha (0.08 larvae/plant) at 7 days after treatment followed by medium and lower dose of E2Y 45 20 SC i.e. @ 37.5ml/ha (0.10 larvae/plant) and 25.0 ml/ha (0.33 larvae/plant). The larval population in these treatments was significantly lower than standard check, Padan 50 SP (2.56 larvae/plant), and untreated control (9.73 larvae/plant). The highest marketable yield (262.89 q/ha) was recorded in E2Y 45 20 SC @ 50.0 ml/ha which was significantly higher than Padan 50 SP (239.65 q/ha). Lowest yields were recorded in untreated control (106.00 q/ha). Conclusively, medium (37.5 ml/ha) and higher (50.0 ml/ha) dose of E2Y 45 20 SC (chlorantraniliprole) significantly reduced the larval population of P. xylostella and increased the cabbage yield

Effect of sulphur supplementation on micronutrients, fatty acids and sulphur use efficiency of soybean seeds

The present study was aimed at finding the influence of different sources and doses of sulphur fertilizers on micronutrient status and oil composition in soybean seeds. Soybean is the major source of edible vegetable oils and high protein seed supplements in the world. Sulphur deficiency causes soybean protein quality to decline and also decreases nitrogen-use efficiency of fertilizers. Soybean is a good source of nutrients which could further be amended with biofortification and use of fertilizers, to meet the nutrient deficiencies. Various limiting factors affect the yield of soybean crop by affecting the yield potential. Sufficient sulphur deficiency is one such limiting factor and have become common all over due to intensive crop systems and higher yielding varieties. Micronutrients play an important role in quality and quantity of soybean yield. Sulphur fertilizers viz gypsum and single super phosphate (SSP) were used at three different doses. Soil analysis have been done to evaluate the fertility status of soil prior to the experiment. Different treatments of sulphur supplementation had significant effect on seed micronutrient accumulation, nitrogen sulphur ratio and fatty acid profile. Sulphur supplementation increased zinc and iron content in mature soybean seeds, however, copper and manganese were found to be least effective. Sulphur supplementation with gypsum @ 20 kgha-1 increased plant height and pods per plant. Increase of oleic acid coincided with the decrease of linoleic acid with sulphur supplementation during both the cropping seasons of study

Structural insights into global mutations in the ligand-binding domain of VAR2CSA and its implications on placental malaria vaccine.

Abstract Placental malaria is a public health burden particularly in Africa as it causes severe symptoms and results in stillbirths or maternal deaths. Plasmodium falciparum protein VAR2CSA drives placental malaria (PM) in pregnant women by adhering to chondroitin sulfate A (CSA) on the placenta. VAR2CSA is a primary vaccine candidate for PM with two vaccines based on it already under clinical trials. The first cryo-EM three-dimensional structure of Pf CSA-VAR2CSA complex revealed crucial interacting residues considered to be highly conserved across P. falciparum strains. In the current study, we have conducted a global sequence analysis of 1,114 VAR2CSA field isolate sequences from more than nine countries across three continents revealing numerous mutations in CSA-binding residues. Further, structural mapping has revealed significant polymorphisms on the ligand binding surfaces. The variants from this limited set of 1,114 sequences highlight the concerns that are vital in current considerations for development of vaccines based on VAR2CSA for placental malaria

Wireless Electricity Theft Detection System Using Zigbee Technology

Wireless electricity theft detection system using ZIGBEE technology present an efficient and less costly way to adulterate the wireless technique used in this research paper. This wireless system is used to overcome the theft of electricity via bypassing the energy meter and hence it also controls the revenue losses and utility of the electricity authorised agency . There is always a contract between the consumer and the supplier that the consumer will pay for the electricity consumed by him. But in India near about 32 % of the electricity is consumed but not paid for it i.e. it is being stolen by the consumer hence the need of a system arises that would overcome this theft of electricity but mostly the electricity is being stolen via bypassing the energy meter hence this system recognises such type of theft of electricity. Mainly this system consists of microcontroller, energy meter and a ZIGBEE module to check f or the theft of electricity and then to send a message to the authorised agency which looks after the electricity consumed. The wireless technique used in this system provides the major advantages such as low power consumption and also the low cost of the ZIGBEE module. This system can also have the advantages that it can also be used to detect the theft of the gas, fuel and oil simply by changing the measurement meter used in this system and excellently the theft can be detected at tables by the authorised agencies

Structural insights into chondroitin sulphate A binding Duffy-binding-like domains from Plasmodium falciparum: implications for intervention strategies against placental malaria

<p>Abstract</p> <p>Background</p> <p>Placental malaria is typified by selective clustering of <it>Plasmodium falciparum </it>in the intervillous blood spaces of the placenta. Sequestration of malaria parasite in the human placenta is mediated by interactions between chondroitin sulphate A (CSA) on the syncytiotrophoblasts and proteins expressed on the surface of infected human erythrocytes. <it>Plasmodium falciparum </it>Erythrocyte Membrane Protein 1 (PfEMP1) encoded by the <it>var2CSA </it>gene is believed to be the main parasite ligand for CSA-mediated placental binding.</p> <p>Methods</p> <p>Extensive sequence and structure comparisons of the various CSA-binding and non-binding DBL domains from the <it>var2CSA </it>gene from A4 and 3D7 strains of <it>P. falciparum </it>were performed. Three-dimensional structural models of various DBL domains were built and analysed with a view to assessing conservation of CSA interaction sites across various DBL domains.</p> <p>Results</p> <p>Each of the six DBL domains from <it>var2CSA </it>are likely to retain the disulfide linkages evident from previously published DBL domain crystal structures. The number of disulfide linkages between the various DBL domains analysed varies from three to seven, of which two are conserved across all DBL domains. The conserved disulfide linkages are distributed within the respective three sub-domains and only one linkage is shared by sub-domains I and II. Major differences between CSA-binding DBL domains are in the loop regions, which tie the alpha helices together, and in variable length terminal extensions. Intriguingly, a crucial loop from A4 DBL 3X which provides the important Gly and Lys residues that chelate the bound sulphate is missing or significantly altered in all other DBL domains that interact with CSA. Further analysis of the proposed sulphate and predicted CSA-binding site indicates either none or very low level of conservation among the critical interacting residues.</p> <p>Conclusion</p> <p>Structural comparisons of the three-dimensional structures of CSA-binding DBL domains indicates that the proposed CSA interaction site on A4 DBL 3X is unlikely to be conserved across the other CSA-binding DBL domains from <it>var2CSA</it>. Therefore, the 4 CSA-binding DBL domains encoded by <it>var2CSA </it>are unlikely to have common architectures to their CSA recognition sites. These structural insights have clear implications in using CSA-binding DBL domains for vaccines against placental malaria as it is proposed that the various CSA-binding DBL domains on <it>var2CSA </it>will recognize their CSA ligands differently.</p

FORMULATION AND EVALUATION OF FAST DISSOLVING TABLETS OF BUSPIRONE USING COPROCESSED SUPERDISINTEGRANTS

Objective: The present study aims to formulate and evaluate Fast dissolving tablet of Buspirone, the drug that is used for management of anxiety, by direct compression method using various Super disintegrants. Methods: Ten formulations (F1-F10) of fast dissolving tablets of Buspirone were prepared by using various Superdisintegrants. The prepared tablets were evaluated for hardness, friability, thickness, drug content uniformity, water absorption, wetting time, and disintegration time and in vitro dissolution study. Results: Among all the formulations, F10 (containing 5 mg of Coprocessed (CS: SSG 1:2) Superdisintegrants) was considered to be the best formulation, which released up to 98% drug in 20 min as compared to a marketed conventional dosage form which dissolves in approx 60 min. The results of stability study of formulation F10 after a period of two months indicated that the formulation was stable. Conclusion: It was concluded that a fast-dissolving tablet of Buspirone containing various Superdisintegrants is better and effective to meet patient compliance

Use of viral promoters in mammalian cell-based bioassays: How reliable?

Cell-based bioassays have been suggested for screening of hormones and drug bioactivities. They are a plausible alternative to animal based methods. The technique used is called receptor/reporter system. Receptor/reporter system was initially developed as a research technique to understand gene function. Often reporter constructs containing viral promoters were used because they could be expressed with very 'high' magnitude in a variety of cell types in the laboratory. On the other hand mammalian genes are expressed in a cell/tissue specific manner, which makes them (i.e. cells/tissues) specialized for specific function in vivo. Therefore, if the receptor/reporter system is to be used as a cell-based screen for testing of hormones and drugs for human therapy then the choice of cell line as well as the promoter in the reporter module is of prime importance so as to get a realistic measure of the bioactivities of 'test' compounds. We evaluated two conventionally used viral promoters and a natural mammalian promoter, regulated by steroid hormone progesterone, in a cell-based receptor/reporter system. The promoters were spliced into vectors expressing enzyme CAT (chloramphenicol acetyl transferase), which served as a reporter of their magnitudes and consistencies in controlling gene expressions. They were introduced into breast cell lines T47D and MCF-7, which served as a cell-based source of progesterone receptors. The yardstick of their reliability was highest magnitude as well as consistency in CAT expression on induction by sequential doses of progesterone. All the promoters responded to induction by progesterone doses ranging from 10(-12 )to 10(-6 )molar by expressing CAT enzyme, albeit with varying magnitudes and consistencies. The natural mammalian promoter showed the most coherence in magnitude as well as dose dependent expression profile in both the cell lines. Our study casts doubts on use of viral promoters in a cell-based bioassay for measuring bioactivities of drugs and hormones for human therapy and suggests caution regardingtranslation in toto, of a research technique as a cell-based bioassay for drug screening