Improved Online Algorithm for Fractional Knapsack in the Random Order Model

The fractional knapsack problem is one of the classical problems in combinatorial optimization, which is well understood in the offline setting. However, the corresponding online setting has been handled only briefly in the theoretical computer science literature so far, although it appears in several applications. Even the previously best known guarantee for the competitive ratio was worse than the best known for the integral problem in the popular random order model. We show that there is an algorithm for the online fractional knapsack problem that admits a competitive ratio of 4.39. Our result significantly improves over the previously best known competitive ratio of 9.37 and surpasses the current best 6.65-competitive algorithm for the integral case. Moreover, our algorithm is deterministic in contrast to the randomized algorithms achieving the results mentioned above

Experimental and theoretical study on bond behavior of GFRP bars in steel fiber reinforced self compacting concrete

To estimate the cracking and the deformational behavior of steel fiber reinforced selfcompacting concrete (SFRSCC) beams reinforced with glass fiber reinforced polymer (GFRP) bars, it is fundamental to understand the interfacial bond behavior of embedded bars. Hence, the evaluation of the bond behavior between GFRP and (SFRSCC) was investigated in this study. A closed-form formulation was derived, adopting a new local bond stress-slip relationship. Furthermore, an experimental program composed of pullout bending tests was carried out in order to assess the influence of the following parameters on the bond behavior: bar diameter, bar surface treatment, embedment length and SFRSCC cover thickness. Finally, a numerical simulation was performed with a FEM-based computer program in order to simulate the bond behavior between GFRP bar and SFRSCC by means of a non-linear bond-slip relationship assigned to the interface finite element. The predictive performance of the theoretical models was appraised by comparing experimental and numerical results

Hyperglycemia selectively increases the expression of cycloxygenase-2 in human aortic endothelial cells

The conversion of arachidonic acid to vasoactive prostanoids including prostacyclin, prostaglandins and tromboxanes is mediated by cycloxygenase (COX). Two isoforms of enzyme have been shown: a constitutive (COX-1) and an inducible form (COX-2). Products of the arachidonic acid metabolism may be involved in the impairment of endothelium-dependent vasodilatation observed both in experimental models and in patients with diabetes mellitus. To determine the effect of hyperglycemia on COX-1 and COX-2 expression, human aortic endothelial cells (HAEC) were exposed to normal (5.5mM) and high (22.2mM) concentrations of glucose for 5 days. Cells were also treated with mannitol (22.2 mM) to rule out an effect due to osmolality changes. COX-1 and COX-2 mRNA and protein expressions were analyzed by Southern and Western blotting, respectively. Treatment with high glucose was associated with a two-fold increase of both COX-2 mRNA (P<0.05) and protein levels (P<0.05), whereas no changes were observed for COX-1. Moreover high concentration of mannitol did not exert any significant effect. The present study demonstrates that both isoforms of COX are normally expressed in HAEC, but only COX-2 was stimulated after exposure to high glucose. The results of the present study may provide molecular basis to understand hyperglycemia-induced endothelial dysfunctio

Ultrasound delivery of Surface Enhanced InfraRed Absorption active gold-nanoprobes into fibroblast cells: a biological study via Synchrotron-based InfraRed microanalysis at single cell level

Ultrasound (US) induced transient membrane permeabilisation has emerged as a hugely promising tool for the delivery of exogenous vectors through the cytoplasmic membrane, paving the way to the design of novel anticancer strategies by targeting functional nanomaterials to specific biological sites. An essential step towards this end is the detailed recognition of suitably marked nanoparticles in sonoporated cells and the investigation of the potential related biological effects. By taking advantage of Synchrotron Radiation fourier transform infrared micro-spectroscopy (SR-microftiR) in providing highly sensitive analysis at the single cell level, we studied the internalisation of a nanoprobe within fibroblasts (NIH-3T3) promoted by low-intensity US. To this aim we employed 20 nm gold nanoparticles conjugated with the IR marker 4-aminothiophenol. The significant Surface Enhanced Infrared Absorption provided by the nanoprobes, with an absorbance increase up to two orders of magnitude, allowed us to efficiently recognise their inclusion within cells. Notably, the selective and stable SR- microftiR detection from single cells that have internalised the nanoprobe exhibited clear changes in both shape and intensity of the spectral profile, highlighting the occurrence of biological effects. Flow cytometry, immunofluorescence and murine cytokinesis-block micronucleus assays confirmed the presence of slight but significant cytotoxic and genotoxic events associated with the US-nanoprobe combined treatments. our results can provide novel hints towards US and nanomedicine combined strategies for cell spectral imaging as well as drug delivery-based therapies

Differential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields

Among different therapeutic applications of Ultrasound (US), transient membrane sonoporation (SP) - a temporary, non-lethal porosity, mechanically induced in cell membranes through US exposure - represents a compelling opportunity towards an efficient and safe drug delivery. Nevertheless, progresses in this field have been limited by an insufficient understanding of the potential cytotoxic effects of US related to the failure of the cellular repair and to the possible activation of inflammatory pathway. In this framework we studied the in vitro effects of very low-intensity US on a human keratinocyte cell line, which represents an ideal model system of skin protective barrier cells which are the first to be involved during medical US treatments. Bioeffects linked to US application at 1 MHz varying the exposure parameters were investigated by fluorescence microscopy and fluorescence activated cell sorting. Our results indicate that keratinocytes undergoing low US doses can uptake drug model molecules with size and efficiency which depend on exposure parameters. According to sub-cavitation SP models, we have identified the range of doses triggering transient membrane SP, actually with negligible biological damage. By increasing US doses we observed a reduced cells viability and an inflammatory gene overexpression enlightening novel healthy relevant strategies

Mid-Infrared Plasmonic Platform Based on n-Doped Ge-on-Si: Molecular Sensing with Germanium Nano-Antennas on Si

CMOS-compatible, heavily-doped semiconductor films are very promising for applications in mid-infrared plasmonic devices because the real part of their dielectric function is negative and broadly tunable in this wavelength range. In this work we investigate n-type doped germanium epilayers grown on Si substrates. We design and realize Ge nanoantennas on Si substrates demonstrating the presence of localized plasmon resonances, and exploit them for molecular sensing in the mid-infrared

Thermoplasmonic Effect of Surface-Enhanced Infrared Absorption in Vertical Nanoantenna Arrays

Thermoplasmonics is a method for increasing temperature remotely using focused visible or infrared laser beams interacting with plasmonic nanopartides. Here, local heating induced by mid-infrared quantum cascade laser illumination of vertical gold-coated nanoanterma arrays embedded into polymer layers is investigated by infrared nanospectroscopy and electromagnetic/thermal simulations. Nanoscale thermal hotspot images are obtained by a phototherrnal scanning probe microscopy technique with laser illumination wavelength tuned at the different plasmonic resonances of the arrays. Spectral analysis indicates that both Joule heating by the metal antennas and surface-enhanced-infrared absorption (SEIRA) by the polymer molecules located in the apical hotspots of the antennas are responsible for thermoplasmonic resonances, that is, for strong local temperature increase. At odds with more conventional planar nanoantennas, the vertical antenna structure enables thermal decoupling of the hotspot at the antenna apex from the heat sink constituted by the solid substrate. The temperature increase was evaluated by quantitative comparision of data obtained with the photothermal expansion technique to the results of electromagnetic/thermal simulations. In the case of strong SEIRA by the C=O bond of poly-methylmethacrylate at 1730 cm(-1), for focused mid-infrared laser power of about 20 mW, the evaluated order of magnitude of the nanoscale temperature increase is of 10 K. This result indicates that temperature increases of order of hundreds of K may he attainable with full mid-infrared laser power tuned at specific molecule vibrational fingerprints

Limiting mechanisms for photon recycling in thin-film GaAs solar cells

Photon recycling mechanisms in single junction thin-film GaAs solar cells are evaluated in this study. Modelling supported by experimentally obtained results is used in order to correlate the reflectance of the cell's rear layers, the photon recycling probability, and the solar cell performance. Solar cells with different top and bottom metallization configurations are produced, and their performance is analyzed from the optical and electrical point of view. It is shown that the photon recycling probability increases with the rear mirror reflectance and solar cell thickness, which results in the increase of the devices open circuit voltage. However, the front grid coverage, usually disregarded in rear mirror focused studies, strongly reduces the photon recycling probability. Furthermore, perimeter and interface recombination hinder the internal radiative efficiency of the solar cells, preventing further increase of the devices' open circuit voltage as a result of improvements of the rear mirror reflectivity. In order to exploit the significant benefit of increased photon recycling probability to the solar cell performance, these limiting mechanisms need to be properly addressed

Experiments and Models for physics learning in primary school

The project “Little scientists in the lab: Experiments & Models for science learning in primary school”, funded by the Ministry of Education and being currently under development in Italy, is addressed to primary school teachers of the Faculty of Science Education and consequently to pupils. This project proposes a “Model-centered Learning Environment” to build pilot activities for teaching and learning science and physics, based on experimental and modeling activities. The approach for teacher training is to assign group tasks that promote learning.We set up a website to support teachers’ school activities to facilitate and promote communication and exchange of materials between teachers and researchers of the University of Modena and Reggio Emilia, as well as between the teachers themselves. In this paper, we illustrate the general features of the project and focus on preliminary results of an in-service training for teachers in fluids and electricity