Carnation Milk Farm

Veterinarians are being called upon more extensively of late to aid commercial enterprises in their problems with diseases of animals and birds. After fourteen years of association with experimental work, teaching and farm practice with land grant colleges, the opportunity was presented the author to act as veterinarian at Carnation Milk Farm and as supervisor of Albers Milling Company feed research carried on at Carnation Milk Farm

Plum pox virus of stone fruits (2000)

"5M1/00NVO.""Penn State College of Agricultural Sciences. Cooperative Extension.

Complete Deletion of \u3ci\u3eWheat Streak Mosaic Virus\u3c/i\u3e HC-Pro: a Null Mutant Is Viable for Systemic Infection

A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the virus titer was reduced 4.5-fold relative to that of the wild type. Collectively, these results indicate that WSMV HC-Pro is dispensable for replication and movement, two essential processes that are disrupted by point and small-insertion mutations introduced into potyvirus HC-Pro

A graduate dormitory for the Massachusetts Institute of Technology

Thesis (M. Arch.)--Massachusetts Institute of Technology, Dept. of Architecture, 1952.Accompanying drawings held by MIT Museum.Includes bibliographical references (leaf 46).by Elton C. Gildow.M.Arch

Modeling Temporal Trends in Aphid Vector Dispersal and Cucumber Mosaic Virus Epidemics in Snap Bean

Cucumber mosaic virus (CMV) has become a major limiting factor in snap bean production in the Great Lakes region of North America, and epidemics have occurred more frequently since the soybean aphid, Aphis glycines Matsumura, was introduced. Major aphid vectors of CMV epidemics were identified by statistically relating their temporal dispersal trends to the incidence of CMV. Alates were monitored weekly using water pan traps in 74 snap bean fields in New York and Pennsylvania from 2002 to 2006. Plants were tested for CMV by ELISA one time during late bloom in 2002 and 2003 and weekly over the season from 2004 to 2006. Principal vectors of CMV included Acyrthosiphon pisum (Harris), A. glycines, Aphis gossypii Glover, and Therioaphis trifolii (Monell). Among these, A. glycines and T. trifolii were likely responsible for severe CMV epidemics because they were among the most abundant species captured, they efficiently transmit CMV, and their dispersal activity was positively correlated with periods when CMV incidence was highest. Moreover, because high numbers of A. glycines and T. trifolii disperse during July and August, snap bean fields planted beyond late June are at risk for infection during early vegetative stages and are subsequently more at risk for yield loss. In contrast, plantings up to late June are less likely to become infected during early developmental stages and should escape yield loss because major vectors are dispersing infrequently. CMV-resistant or tolerant snap bean varieties should be planted after late June to reduce the risk of yield los

Conditional Facilitation of an Aphid Vector, Acyrthosiphon pisum, by the Plant Pathogen, Pea Enation Mosaic Virus

Plant pathogens can induce symptoms that affect the performance of insect herbivores utilizing the same host plant. Previous studies examining the effects of infection of tic bean, Vicia faba L. (Fabales: Fabaceae), by pea enation mosaic virus (PEMV), an important disease of legume crops, indicated there were no changes in the growth and reproductive rate of its primary vector the pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae). Here, we report the results of laboratory experiments investigating how A. pisum responded to PEMV infection of a different host plant, Pisum sativum L., at different stages of symptom development. Aphid growth rate was negatively related to the age of the host plant, but when they were introduced onto older plants with well-developed PEMV symptoms they exhibited a higher growth rate compared to those developing on uninfected plants of the same age. In choice tests using leaf discs A. pisum showed a strong preference for discs from PEMV-infected peas, probably in response to visual cues from the yellowed and mottled infected leaves. When adults were crowded onto leaves using clip-cages they produced more winged progeny on PEMV-infected plants. The results indicate that PEMV produces symptoms in the host plant that can enhance the performance of A. pisum as a vector, modify the production of winged progeny and affect their spatial distribution. The findings provide further evidence that some insect vector/plant pathogen interactions could be regarded as mutualistic rather than commensal when certain conditions regarding the age, stage of infection and species of host plant are met

Multiple models guide strategies for agricultural nutrient reductions

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/136504/1/fee1472_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136504/2/fee1472-sup-0008-WebTable7.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136504/3/fee1472-sup-0004-WebTable3.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136504/4/fee1472.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136504/5/fee1472-sup-0006-WebTable5.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136504/6/fee1472-sup-0002-WebTable1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136504/7/fee1472-sup-0005-WebTable4.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136504/8/fee1472-sup-0007-WebTable6.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136504/9/fee1472-sup-0003-WebTable2.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136504/10/fee1472-sup-0001-WebFig1.pd

Proteomic Insights into the Hidden World of Phloem Sap Feeding

The physical interface between a phloem-feeding insect and its host plant is a single cell buried deep within the plant tissue. As such, the molecular interactions between these notorious agricultural pests and the crop plants upon which they feed are diffi cult to study. ‘Omic’ technologies have proved crucial in revealing some of the fascinating detail of the molecular interplay between these partners. Here we review the role of proteomics in identifying putative components of the secreted saliva of phloem-feeding insects, particularly aphids, and discuss the limited knowledge concerning the function of these proteins