Trypanosomes are monophyletic: evidence from genes for glyceraldehyde phosphate dehydrogenase and small subunit ribosomal RNA.

The genomes of Trypanosoma brucei, Trypanosoma cruzi and Leishmania major have been sequenced, but the phylogenetic relationships of these three protozoa remain uncertain. We have constructed trypanosomatid phylogenies based on genes for glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) and small subunit ribosomal RNA (SSU rRNA). Trees based on gGAPDH nucleotide and amino acid sequences (51 taxa) robustly support monophyly of genus Trypanosoma, which is revealed to be a relatively late-evolving lineage of the family Trypanosomatidae. Other trypanosomatids, including genus Leishmania, branch paraphyletically at the base of the trypanosome clade. On the other hand, analysis of the SSU rRNA gene data produced equivocal results, as trees either robustly support or reject monophyly depending on the range of taxa included in the alignment. We conclude that the SSU rRNA gene is not a reliable marker for inferring deep level trypanosome phylogeny. The gGAPDH results support the hypothesis that trypanosomes evolved from an ancestral insect parasite, which adapted to a vertebrate/insect transmission cycle. This implies that the switch from terrestrial insect to aquatic leech vectors for fish and some amphibian trypanosomes was secondary. We conclude that the three sequenced pathogens, T. brucei, T. cruzi and L. major, are only distantly related and have distinct evolutionary histories

Re‐emitted positron spectroscopy of cobalt and nickel silicide films

The techniques of re‐emitted positron spectroscopy (RPS) have been employed in the first systematic investigation of the positronic properties of the various stoichiometric phases (M2Si, MSi, and MSi2) of Co and Ni silicide films grown in situ on Si substrates. The positron work function is found to be negative for all of the different phases; thus implanted positrons may be re‐emitted. The energy of the re‐emitted positrons is found to have a surprisingly large variation for the different phases. This feature should provide the image contrast necessary to observe each phase on a microscopic scale using the positron re‐emission microscope (PRM). The positron deformation potential, E+d≡V(∂Σ/∂V), was determined for CoSi2 films; it can be used to estimate the size of the positron diffusion constant, which is found to be comparable to that of other metals. Thus the short positron diffusion length (of order 150 Å) determined from depth‐profiling measurements of CoSi2 films must be a result of positron trapping in either the film or at the interface with the Si substrate. RPS results considered as a function of film thickness support the conclusion that defects in the film (misfit dislocations and/or vacancies) represent the major source of positron trapping.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87603/2/264_1.pd

Ovarian hormones and pituitary responsiveness to gonadotrophin releasing hormone in mice

In the present study the role of progesterone and oestradiol in modulating the responsiveness at the anterior pituitary gland to gonadotrophin releasing hormone (GN-RH) was investigated by measuring the release of luteinizing hormone (LH) in ovariectomised mice which had been pretreated with these steroids.A significant release of LH was seen in the animals receiving GN-RH. Pretreatment with oestrogen and progesterone depressed LH levels in the animals which did not receive GN-RH, and resulted in a larger release of LH in the animals receiving GN-RH in comparison with the control group pretreated with oil

Positron tunneling microscopy

A new technique for analyzing thin film growth processes, called positron tunneling microscopy (PTM), is proposed as an extension of the recently developed positron reemission microscope. The unique feature of a PTM is that image contrast is provided by the exponential reemission probability for positrons tunneling through thin-film overlayers that present an energy barrier to reemission. Results of positron tunneling experiments show that PTM should have monolayer thickness resolution to processes that locally affect either the tunneling barrier's width, such as islanding and subsurface roughness, or the barrier's energy, such as lattice strain in pseudomorphic growth and compositional mixing in interdiffusion alloying. In the case of these latter effects where there may be no topological contrasts at all, experimental results are discussed in greater detail. Comparisons of PTM with existing electron microscopies are presented where appropriate.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/28554/1/0000356.pd

A study protocol for a randomised crossover study evaluating the effect of diets differing in carbohydrate quality on ileal content and appetite regulation in healthy humans

A major component of the digesta reaching the colon from the distal ileum is carbohydrate. This carbohydrate is subject to microbial fermentation and can radically change bacterial populations in the colon and the metabolites they produce, particularly short-chain fatty acids (SCFA). However, very little is currently known about the forms and levels of carbohydrate in the ileum and the composition of the ileal microbiota in humans. Most of our current understanding of carbohydrate that is not absorbed by the small intestine comes from ileostomy models, which may not reflect the physiology of an intact gastrointestinal tract. We will investigate how ileal content changes depending on diet using a randomised crossover study in healthy humans. Participants will be inpatients at the research facility for three separate 4-day visits. During each visit, participants will consume one of three diets, which differ in carbohydrate quality: 1) low-fibre refined diet; 2) high-fibre diet with intact cellular structures; 3) high-fibre diet where the cellular structures have been disrupted (e.g. milling, blending). On day 1, a nasoenteric tube will be placed into the distal ileum and its position confirmed under fluoroscopy. Ileal samples will be collected via the nasoenteric tube and metabolically profiled, which will determine the amount and type of carbohydrate present, and the composition of the ileal microbiota will be measured. Blood samples will be collected to assess circulating hormones and metabolites. Stool samples will be collected to assess faecal microbiota composition. Subjective appetite measures will be collected using visual analogue scales. Breath hydrogen will be measured in real-time as a marker of intestinal fermentation. Finally, an continuous fermentation model will be inoculated with ileal fluid in order to understand the shift in microbial composition and SCFA produced in the colon following the different diets. ISRCTN11327221. [Abstract copyright: Copyright: © 2019 Byrne CS et al.

An overview of the Michigan Positron Microscope Program

An overview of the Michigan Positron Microscope Program is presented with particular emphasis on the second generation microscope that is presently near completion. The design and intended applications of this microscope will be summarized.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87602/2/391_1.pd

Visualization of microbe-dietary remnant interactions in digesta from pigs, by fluorescence in situ hybridization and staining methods; effects of a dietary arabinoxylan-rich wheat fraction

This study investigated how the addition of a specific hydrocolloid, arabinoxylan (AX), to the pig diets containing red meat, affected the gut microbiota in terms of changes to the bacterial community dynamics. Fluorescence in situ hybridization (FISH) was used to enumerate both broad groups and particular bacterial species, and showed systematic differences in pigs fed AX. In addition, the role of bacteria in the fermentation of dietary substrates was studied by visualizing direct associations between the bacteria and substrate particles. To achieve this, novel methods of combining histological staining or enzymatic labelling with FISH protocols were established. In this way, undigested and/or unfermented remnants of meat, endogenous mucin, and several plant cell wall hydrocolloids (cellulose, lignin and arabinoxylan) were distinguished, together with large intestine microbiota under brightfield and/or confocal laser scanning microscopy (CLSM). It was evident that no apparent direct associations occurred between specific classes or groups of bacteria and meat, mucin, lignin or AX remnants. In contrast, bacteria belonging to Clostridium clusters XIVa and XIVb formed a strong (P < 0.01) direct association with cellulose remnants in the pig digesta in the caecum

Structural changes in glassy polycarbonate induced by cyclic stresses

Glassy polycarbonate was subjected to cyclic stresses and the changes in its structure were studied by DSC, positronium annihilation lifetime spectroscopy (PALS), and SAXS. On increased exposure to cyclic loading, the enthalpy overshoot near Tg increased in a manner similar to increasing physical aging. However, the `hole' size as revealed by PALS increased, unlike physical aging. SAXS reveals an increase in the short range order and the amplitude of density fluctuation. These results are consistent with both the DSC and the PALS results. We conclude that mechanical loading does cause changes in the glassy structure.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29285/1/0000345.pd

Suburban ethnicities: Home as the site of interethnic conviviality and racism

This is the final version. Available on open access from Wiley via the DOI in this recordThis article explores the ways in which the white working class residents of a suburban English town reflect on their relationships with their British Asian Pakistani Muslim neighbours. Its focus is on how everyday constructions of home become sites for the intermingling of discourses of intercultural conviviality and racism. My contention is that the idea of home has not yet been given the detailed critical attention that it deserves in the sociological literature on everyday manifestations of multiculturalism, conviviality and racism. My supposition is that a special focus on the idea of home as the site of conviviality offers a productive avenue to analyse how intercultural relationships are formed and how the norms of neighbourliness are thought to break down, opening a space for commonplace racialized and racist stereotypes to take hold. The idea of home is central to the rhythm and landscape of the English suburbs. It conjures-up the idea of a uniform and aspirational white space. Drawing on this imaginary of home, I shall trace how ‘white working class’ ‘English’, ‘Scottish’ and ‘Anglo-Italian’ residents’ everyday constructions of home become embroiled with their relationships with their British Asian Pakistani Muslim neighbours.Economic and Social Research Council (ESRC