Problems Affecting Labor

Much experimental work has been devoted in comparing the folding behavior of proteins sharing the same fold but different sequence. The recent design of proteins displaying very high sequence identities but different 3D structure allows the unique opportunity to address the protein-folding problem from a complementary perspective. Here we explored by ℙ-value analysis the pathways of folding of three different heteromorphic pairs, displaying increasingly high-sequence identity (namely, 30%, 77%, and 88%), but different structures called G A (a 3-α helix fold) and G B (an α/β fold). The analysis, based on 132 site-directed mutants, is fully consistent with the idea that protein topology is committed very early along the pathway of folding. Furthermore, data reveals that when folding approaches a perfect two-state scenario, as in the case of the G A domains, the structural features of the transition state appear very robust to changes in sequence composition. On the other hand, when folding is more complex and multistate, as for the G Bs, there are alternative nuclei or accessible pathways that can be alternatively stabilized by altering the primary structure. The implications of our results in the light of previous work on the folding of different members belonging to the same protein family are discussed

Direction-Dependent Polarised Primary Beams in Wide-Field Synthesis Imaging

The process of wide-field synthesis imaging is explored, with the aim of understanding the implications of variable, polarised primary beams for forthcoming Epoch of Reionisation experiments. These experiments seek to detect weak signatures from redshifted 21cm emission in deep residual datasets, after suppression and subtraction of foreground emission. Many subtraction algorithms benefit from low side-lobes and polarisation leakage at the outset, and both of these are intimately linked to how the polarised primary beams are handled. Building on previous contributions from a number of authors, in which direction-dependent corrections are incorporated into visibility gridding kernels, we consider the special characteristics of arrays of fixed dipole antennas operating around 100-200 MHz, looking towards instruments such as the Square Kilometre Array (SKA) and the Hydrogen Epoch of Reionization Arrays (HERA). We show that integrating snapshots in the image domain can help to produce compact gridding kernels, and also reduce the need to make complicated polarised leakage corrections during gridding. We also investigate an alternative form for the gridding kernel that can suppress variations in the direction-dependent weighting of gridded visibilities by 10s of dB, while maintaining compact support.Comment: 15 pages, 4 figures. Accepted for publication in JA

How Much Longer Will it Take? A Ten-year Review of the Implementation of United Nations General Assembly Resolutions 61/105, 64/72 and 66/68 on the Management of Bottom Fisheries in Areas Beyond National Jurisdiction

The United Nations General Assembly (UNGA) in 2002 adopted the first in a series of resolutions regarding the conservation of biodiversity in the deep sea. Prompted by seriousconcerns raised by scientists, non-governmental organizations (NGOs) and numerous States,these resolutions progressively committed States to act both individually and through regional fishery management organizations (RFMOs) to either manage bottom fisheries in areas beyond national jurisdiction to prevent significant adverse impacts on deep-sea species, ecosystems and biodiversity or else prohibit bottom fishing from taking place.Ten years have passed since the adoption of resolution 61/105 in 2006, calling on States to take a set of specific actions to manage bottom fisheries in areas beyond national jurisdiction to protect vulnerable marine ecosystems (VMEs) from the adverse impacts of bottom fishing and ensure the sustainability of deep-sea fish stocks. Despite the considerable progress by some RFMOs, there remain significant gaps in the implementation of key elements and commitments in the resolutions. The Deep Sea Conservation Coalition (DSCC) has prepared this report to assist the UNGA in its review in 2016 and to address the following question: How effectively have the resolutions been implemented

The last ten years of research at Tarquinia

The Centro di Ricerca Coordinata ‘ProgettoTarquinia’ of the Università degli Studi di Milano is a LERU (League of European Research Universities) exemplary interdisciplinary research project that involves groups from the Università Statale di Milano (Archaeology, Information and Communication Technologies, Geoarchaeology, Palaeoanthropology), the Politecnico di Milano (Architecture and Topography) and bridges the gap between soft and hard sciences. This project stems from the ‘Progetto Tarquinia’ conceived by Maria Bonghi Jovino in 1982. During the last ten years, our integrated system of tools and services, supported by ICTs (ArchMatrix), through which multidisciplinary domain experts can examine all the typologies of data of a given culture, has made it possible to concentrate on the links between data-sources focusing on the recurrence of association rates within different aspects of material evidence and phenomena. The fields of application of our methodology in the domain of archaeology and epigraphy are multifaceted as regards the inside and outside connections of the Tarquinian heritage, whose necropolis with the famous painted tombs is a UNESCO World Heritage Site. Research includes areas of the Civita plateau: the ‘monumental complex’, the Ara della Regina sanctuary, fortifications, and archaeological sites previously explored. In the past ten years, research in the necropolis (roughly 6,000 tombs, of which 400 are painted) and in the surrounding territory has also been implemented and has produced the complete corpus of the painted tombs of Tarquinia. Our holistic approach encompasses archaeological analysis of small (mobile finds), medium (archaeological contexts) and large scale (territory and landscape) architectural analysis and applications for integrated solutions for the cultural heritage, including the first bilingual Virtual Museum dedicated to an Etruscan city

Speeding up Cylindrical Algebraic Decomposition by Gr\"obner Bases

Gr\"obner Bases and Cylindrical Algebraic Decomposition are generally thought of as two, rather different, methods of looking at systems of equations and, in the case of Cylindrical Algebraic Decomposition, inequalities. However, even for a mixed system of equalities and inequalities, it is possible to apply Gr\"obner bases to the (conjoined) equalities before invoking CAD. We see that this is, quite often but not always, a beneficial preconditioning of the CAD problem. It is also possible to precondition the (conjoined) inequalities with respect to the equalities, and this can also be useful in many cases.Comment: To appear in Proc. CICM 2012, LNCS 736

Unfinished Business: a Review of the Implementation of the Provisions of United Nations General Assembly Resolutions 61/105 and 64/72, Related to the Management of Bottom Fisheries in Areas Beyond National Jurisdiction

In 2006 the General Assembly adopted resolution 61/105, based on a compromise proposal offered by deep-sea fishing nations, which committed States and regional fisheries management organisations [RFMOs] to take specific measures to protect vulnerable marine ecosystems [VMEs] from the adverse impacts of bottom fisheries in the high seas and to ensure the longterm sustainability of deep-sea fish stocks. These measures included conducting impact assessments to determine whether significant adverse impacts[SAIs] to VMEs would occur, managing fisheries to prevent SAIs on VMEs, and closing areas of the high seas to bottom fishing where VMEs are known or likely to occur, unless regulations are in place to prevent SAIs and to manage sustainably deep-sea fish stocks. Based on a review in 2009 of the actions taken by States and RFMOS, the UNGA adoptedresolution 64/72 that reaffirmed resolution 61/105 and strengthened the call for action through committing States, inter alia, to ensure that vessels do not engage in bottom fishing until impact assessments have been carried out and to not authorise bottom fishing activities until the measures in resolutions 64/72 and 61/105 have been adopted andimplemented

Detection and isolation of circulating tumor cells with single-cell resolution: A successful lab-on-a-chip device

This paper was presented at the 3rd Micro and Nano Flows Conference (MNF2011), which was held at the Makedonia Palace Hotel, Thessaloniki in Greece. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, Aristotle University of Thessaloniki, University of Thessaly, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute.This paper presents the unique features of DEPArray™ an automated system enabling image-based cell sorting with single-cell resolution and describes its potential application in the field of oncology