Serosurvey of schmallenberg virus infection in sheep in Abruzzo, Italy : short report

Infection with Schmallenberg virus (SBV) causes congenital musculoskeletal and vertebral malformations as well as neurological failures in fetuses of several ruminant species. In this study 1038 sheep samples from 10 flocks in the provinces of Chieti, Teramo and Pescara in Italy have been tested for antibodies against SBV by ELISA test. The purpose of the study was to ascertain the extent of SBV infections in sheep in Italy. The results of the ELISA test identified at least one positive animal in 9 of the 10 sheep flocks tested, and a mean within-flock prevalence of 8.57%. Furthermore, large variability of positive animals between flocks was observed (0 and 42.5%). These results demonstrate that SBV was endemic in this region and there could be is a risk of novel SBV infections in the following lambing season, raising serious concerns about its so rapid and pervasive spread

KCNQ1 suppression-replacement gene therapy in transgenic rabbits with type 1 long QT syndrome.

BACKGROUND AND AIMS Type 1 long QT syndrome (LQT1) is caused by pathogenic variants in the KCNQ1-encoded Kv7.1 potassium channels, which pathologically prolong ventricular action potential duration (APD). Herein, the pathologic phenotype in transgenic LQT1 rabbits is rescued using a novel KCNQ1 suppression-replacement (SupRep) gene therapy. METHODS KCNQ1-SupRep gene therapy was developed by combining into a single construct a KCNQ1 shRNA (suppression) and an shRNA-immune KCNQ1 cDNA (replacement), packaged into adeno-associated virus serotype 9, and delivered in vivo via an intra-aortic root injection (1E10 vg/kg). To ascertain the efficacy of SupRep, 12-lead electrocardiograms were assessed in adult LQT1 and wild-type (WT) rabbits and patch-clamp experiments were performed on isolated ventricular cardiomyocytes. RESULTS KCNQ1-SupRep treatment of LQT1 rabbits resulted in significant shortening of the pathologically prolonged QT index (QTi) towards WT levels. Ventricular cardiomyocytes isolated from treated LQT1 rabbits demonstrated pronounced shortening of APD compared to LQT1 controls, leading to levels similar to WT (LQT1-UT vs. LQT1-SupRep, P < .0001, LQT1-SupRep vs. WT, P = ns). Under β-adrenergic stimulation with isoproterenol, SupRep-treated rabbits demonstrated a WT-like physiological QTi and APD90 behaviour. CONCLUSIONS This study provides the first animal-model, proof-of-concept gene therapy for correction of LQT1. In LQT1 rabbits, treatment with KCNQ1-SupRep gene therapy normalized the clinical QTi and cellular APD90 to near WT levels both at baseline and after isoproterenol. If similar QT/APD correction can be achieved with intravenous administration of KCNQ1-SupRep gene therapy in LQT1 rabbits, these encouraging data should compel continued development of this gene therapy for patients with LQT1

Entrepreneurial Human Capital, Complementary Assets, and Takeover Probability

Gaining access to technologies, competencies, and knowledge is observed as one of the major motives for corporate mergers and acquisitions. In this paper we show that a knowledge-based firm's probability of being a takeover target is influenced by whether relevant specific human capital aimed for in acquisitions is directly accumulated within a specific firm or is bound to its founder or manager owner. We analyze the incentive effects of different arrangements of ownership in a firm's assets in the spirit of the Grossman-Hart-Moore incomplete contracts theory of the firm. This approach highlights the organizational significance of ownership of complementary assets. In a small theoretical model we assume that the entrepreneur's specific human capital, as measured by the patents they own, and the physical assets of their firm are productive only when used together. Our results show that it is not worthwhile for an acquirer to purchase the alienable assets of this firm due to weakened incentives for the initial owner. Regression analysis using a hand collected dataset of all German IPOs in the period from 1997 to 2006 subsequently provides empirical support for this prediction. This paper adds to previous research in that it puts empirical evidence to the Grossman-Hart-Moore framework of incomplete contracts or property rights respectively. Secondly, we show that relevant specific human capital that is accumulated by a firm's founder or manager owner significantly decreases that firm's probability of being a takeover target

Polychlorinated biphenyls and organochlorine pesticide levels in tissues of Caretta caretta from the Adriatic Sea

n/

An Object Deformation-Agnostic Framework for Human-Robot Collaborative Transportation

In this study, an adaptive object deformability-agnostic human-robot collaborative transportation framework is presented. The proposed framework enables to combine the haptic information transferred through the object with the human kinematic information obtained from a motion capture system to generate reactive whole-body motions on a mobile collaborative robot. Furthermore, it allows rotating the objects in an intuitive and accurate way during co-transportation based on an algorithm that detects the human rotation intention using the torso and hand movements. First, we validate the framework with the two extremities of the object deformability range (i.e., purely rigid aluminum rod and highly deformable rope) by utilizing a mobile manipulator which consists of an Omni-directional mobile base and a collaborative robotic arm. Next, its performance is compared with an admittance controller during a co-carry task of a partially deformable object in a 12-subjects user study. Quantitative and qualitative results of this experiment show that the proposed framework can effectively handle the transportation of objects regardless of their deformability and provides intuitive assistance to human partners. Finally, we have demonstrated the potential of our framework in a different scenario, where the human and the robot co-transport a manikin using a deformable sheet. Note to Practitioners - Transportation of objects which requires the cooperation of multiple partners, is a common task in industrial settings such as factories and warehouses. The existing human-robot collaboration solutions for this task have focused only on purely rigid objects, although deformable objects need to be carried frequently in real-world applications. In this paper, we introduce a human-robot collaborative transportation framework that can handle objects with different deformability ranging from purely rigid to highly deformable. In particular, the proposed framework generates whole-body movements on a mobile collaborative robot by combining of the haptic information transmitted through the object and the human motion information obtained from a motion capture system. Moreover, the framework includes an intuitive way to rotate the object during the execution based on human hand and torso motion. The results of the experiments where objects with various deformability characteristics were transported in collaboration with a mobile manipulator demonstrated the high potential of the proposed approach in a laboratory setting. In the future, we plan to employ a less expensive vision-based human motion tracking system instead of the IMU-based system used in this study. With this change, we will be able to eliminate the need for wearable sensors from the framework presented, which would enhance its usability in real-world scenarios