Regulation of gastric acid secretion in situ by an endogenous activator protein: Studies with activator-specific antibody

Monospecific polyclonal antibody was raised against a homogenous preparation of endogenous activator protein (HAF) for the gastric H+,K+-ATPase system. Antibody was used to assess the regulatory role of the HAF in gastric acid secretion by isolated rabbit glands in situ. Immunohistochemical studies revealed aredistribution of theHAFtowards discrete intracellular zones following stimulation of the glands with histamine. The antibody, when inserted into the stimulated gastric glands by digitonin permeabilization, could effectively block the acid forming ability of the cells. The data offers, for the first time, some concrete in situ evidence for the role of the HAF as an intracellular regulator of gastric H+ transport .The manuscript was written (in the early 1990) soon after the data were collected, but never submitted to a journal with the hope of getting better gel picture and fluorescent micrograph which did not materialize due to unavoidable circumstances

Development and Evaluation of an Order-N Formulation for Multi-Flexible Body Space Systems

This paper presents development of a generic recursive Order-N algorithm for systems with rigid and flexible bodies, in tree or closed-loop topology, with N being the number of bodies of the system. Simulation results are presented for several test cases to verify and evaluate the performance of the code compared to an existing efficient dense mass matrix-based code. The comparison brought out situations where Order-N or mass matrix-based algorithms could be useful

Adsorption of Acetaldehyde, Propionaldehyde, and Butyraldehyde on Silica Gel and Molecular Sieve-13x

Chemical Engineerin

Three dimensional modelling and optimisation of multistage collectors

Multistage collectors are commonly used in travelling wave tubes (TWTs), klystrons, gyrotrons and inductive output tubes (IOTs). A dc beam transfers some of its energy to the input RF wave during its travel through the interaction circuit. The purpose of a collector is to recover most of the remaining dc power from the spent beam and thereby increase both the collector and the overall efficiency. Secondary electrons play a detrimental role in collector performance. Use of the asymmetric collector geometries and application of a magnetic field in the collector region have proved to be effective in recapturing the secondaries. A fully three-dimensional simulator LKOBRA (MF) – the mainframe version of Lancaster KOBRA, has been developed at Lancaster University; this is capable of simulating multistage collectors including the effects of secondary electron emission and magnetic field. It is based on KOBRA3-INP1 but has been modified and improved together with the pre- and post-processors of the package. Efficiency is an important parameter in space applications of microwave tubes so it is always desirable to optimise the collector performance to maximise the overall tube efficiency. As a first step the potentials at the collector electrodes are optimised to achieve the maximum possible theoretical efficiency. A computer code based on the well-known enumerative technique has been developed for this purpose. In the next step the geometry of the collector electrodes is optimised using an automated design package that is based on a genetic algorithm. The genetic algorithm creates a new geometry through a search procedure that works from a population of possible geometries. A new set of geometries is generated using three basic operators namely reproduction, crossover and mutation. The collector efficiency is used as the fitness parameter in the genetic algorithm that produces a new population of geometries. This package has been used to optimise the efficiency of a 4-stage symmetric collector and a 2-stage asymmetric collector

Hydrogen Storage in Nano-Phase Diamond at High Temperature and Its Release

The objectives of this proposed research were: 91) Separation and storage of hydrogen on nanophase diamonds. It is expected that the produced hydrogen, which will be in a mixture, can be directed to a nanophase diamond system directly, which will not only store the hydrogen, but also separate it from the gas mixture, and (2) release of the stored hydrogen from the nanophase diamond

In Silico Molecular Insights on the Structure-Function Aspects of ACC Deaminase of a Non-Pathogenic Klebsiella Pneumoniae

Bacterial 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) is known to involve in breaking down the 1-aminocyclopropane-1-carboxylic acid (ACC), immediate precursor of ethylene, into a-ketobutyrate and ammonia. This is required when stress (biotic or abiotic) induced ethylene content is accelerated in plant cells resulting in reduction in plant biomass and yield. Klebsiellapneumoniae, although previously considered only as a pathogenic bacteria, there are some strains reported till date to prove it as a plant growth promoting bacteria (PGPB). ACCD activity has reported from the said strain but the present study is emphasized on its molecular proteomic structures and functions studied in silico. The present work revealed that the ACCD of K. pneumoniae is a 36.5 kDatetrameric stable protein found in intracellular condition. The phylogenetic analysis clearly depicts its similarity with several other ACCD reported from different bacterial genera. The structure-function insight would definitely help future researchers in designing wet lab as well as dry lab experiments

A Service Learning Collaborative to Build a Sustainable Enterprise for Underprivileged Women (SEuW)

The goal of this ongoing service-learning project is to step outside the traditional classroom to engage fashion and textile students in a real world production experience, thus learning about the design and manufacturing process and culture through their activities. The skills and knowledge that the students are developing can go way beyond the traditional monetary manufacturing/retail outcome objectives, touching and influencing people\u27s lives with a global sustainable perspective. A mutually beneficial strategic collaborative effort was organized between an international non-governmental organization (NGO), graduate and undergraduate students at a US University, a large multinational company with extensive ties to Indian manufacturing and the US retail market, and a grassroots women\u27s manufacturing group in Jaipur, India. A number of goals were developed to help support a group of poor and disadvantaged women to become self-sufficient and provide for their families\u27 basic needs, through this textiles-apparel service-learning project

Gestational diabetes: How risky are the mothers of rural Bengal, India

Early detection of gestational diabetes in antenatal mothers can improve both pregnancy and fetal outcome. A descriptive, cross-sectional study was conducted to find out the magnitude of gestational diabetes by selective screening using “American Diabetes Association (ADA) risk approach strategy” and distribution of risk factors of gestational diabetes among the mothers attending the antenatal clinic of Singur Rural Hospital. Pregnant women with gestational age between 24-28 weeks were interviewed using a predesigned schedule adapted from American Diabetes Association and WHO guidelines and their clinical and obstetrical examination was done. Mothers identified with at least 1 risk factor were advised for screening by Glucose Challenge test (GCT). Those with a positive result were confirmed by Glucose Tolerance Test. Out of 625 antenatal mothers, majority i.e. 60.32% of the mothers were exposed to low risk for developing gestational diabetes. Among 248 (39.68%) mothers who had at least one risk factor, 20.56% were GCT positive amounting to 8.16% of the total population. 11.69% of the mothers with positive risk factors were GTT positive amounting to 56.86% of GCT positive mothers. This accounted for 4.64% of the total study population. Thus this method of preliminary screening for risk factors of gestational diabetes undertaken in all antenatal mothers followed by confirmatory testing in those found to be risk factor positive can provide a feasible alternative in increasing the yield for detection of gestational diabetes articularly in a low resource setting