The Molecular Architecture of the Extracellular Hemoglobin of Ophelia bicornis: Analysis of Individual Molecules

The dimensions and shape of extracellular hemoglobin molecules of the marine worm, Ophelia bicornis, were determined by electron microscopy using negative staining and embedding in aurothioglucose. The typical averaged double-layered hexagon has a diameter of 26.5 nm and a height of 18 nm. The three-dimensional reconstruction of the negatively stained samples, using random conical tilting, showed about 60% of the original height of the molecule due to flattening and incomplete embedding in stain. In contrast to this, the specimen embedded in aurothioglucose showed no flattening. The three-dimensional reconstructions agree with the structure found in a previous study on 2-D crystalline arrays. In particular, the main subunit shows more than three mass centers

The evolution of extracellular hemoglobins of annelids, vestimentiferans, and pogonophorans

The evolution of extracellular hemoglobins of annelids, vestimentiferans, and pogonophorans was investigated by applying cladistic and distance-based approaches to reconstruct the phylogenetic relationships of this group of respiratory pigments. We performed this study using the aligned sequences of globin and linker chains that are the constituents of these complex molecules. Three novel globin and two novel linker chains of Sabella spallanzanii described in an accompanying paper (Pallavicini, A., Negrisolo, E., Barbato, R., Dewilde, S., Ghiretti-Magaldi, A., Moens, L., and Lanfranchi, G. (2001) J. Biol. Chem. 276, 26384--26390) were also included. Our results allowed us to test previous hypotheses on the evolutionary pathways of these proteins and to formulate a new most parsimonious model of molecular evolution. According to this novel model, the genes coding for the polypeptides forming these composite molecules were already present in the common ancestor of annelids, vestimentiferans, and pogonophorans

The primary structure of globin and linker chains from the chlorocruorin of the polychaete Sabella spallanzanii

Annelid hemoglobins are organized in a very complex supramolecular network of interacting polypeptides, the structure of which is still not wholly resolved. We have separated by two-dimensional electrophoresis the 4-MDa chlorocruorin of Sabella spallanzanii and identified its components by amino-terminal sequencing. This work reveals a high rate of heterogeneity of constituent chains in a single animal as well as in the Sabella population. Using a cDNA library prepared from the hematopoietic tissue of this worm, we have isolated and fully sequenced most globin and linker cDNAs. The primary structure features of these polypeptides have been characterized by comparison with model globin and linker sequences