Preliminary Study of Plants Used in Ethnoveterinary Medicine in Tunisia and in Italy

Background: A survey relative to the use of plants for the cure of animals in Tunisia was conducted in order to make a comparison with the same species (or similar ones) in central and southern Italy.Materials and methods: available bibliographical data both for Italy and for Tunisia were consulted.Results: Thirty-nine plants, representing 22 families, used in Tunisia in ethnoveterinary medicine were reported, and comparisons made with close species used in Central and Southern Italy. Seven of the 39 species (about the 18% of the total) are not present in Italian flora. Fourteen of the 39 species (35% of the total) are also used in Italy. Camelidae (dromedaries and camels) are the most valuable types of domestic animals cured in Tunisia, but ovines, horses, bulls, dogs are also treated. Some uses coincide with those existing in different Italian regions. The plants used are the most common and most easily found in these areas.Conclusion: The present study confirms the convergence in ethnoveterinary medicine between Tunisia and Italy, even if it appears less significant than in human ethnobotany. Further studies are required in areas of Tunisia that have not yet been studied, in order to get the possibility of an evaluation of active compounds.Key words: Ethnoveterinary medicine; Plants; Tunisia; Italy. Introductio

User-centric Privacy Engineering for the Internet of Things

User privacy concerns are widely regarded as a key obstacle to the success of modern smart cyber-physical systems. In this paper, we analyse, through an example, some of the requirements that future data collection architectures of these systems should implement to provide effective privacy protection for users. Then, we give an example of how these requirements can be implemented in a smart home scenario. Our example architecture allows the user to balance the privacy risks with the potential benefits and take a practical decision determining the extent of the sharing. Based on this example architecture, we identify a number of challenges that must be addressed by future data processing systems in order to achieve effective privacy management for smart cyber-physical systems.Comment: 12 Page

Antimutagenic and free radical scavenger effects of leaf extracts from Accacia salicina

<p>Abstract</p> <p>Background</p> <p>Three extracts were prepared from the leaves of <it>Accacia salicina</it>; ethyl acetate (EA), chloroform (Chl) and petroleum ether (PE) extracts and was designed to examine antimutagenic, antioxidant potenty and oxidative DNA damage protecting activity.</p> <p>Methods</p> <p>Antioxidant activity of <it>A. salicina </it>extracts was determined by the ability of each extract to protect against plasmid DNA strand scission induced by hydroxyl radicals. An assay for the ability of these extracts to prevent mutations induced by various oxidants in <it>Salmonella typhimurium </it>TA102 and TA 104 strains was conducted. In addition, nonenzymatic methods were employed to evaluate anti-oxidative effects of tested extracts.</p> <p>Results</p> <p>These extracts from leaf parts of <it>A. salicina </it>showed no mutagenicity either with or without the metabolic enzyme preparation (S9). The highest protections against methylmethanesulfonate induced mutagenicity were observed with all extracts and especially chloroform extract. This extract exhibited the highest inhibitiory level of the Ames response induced by the indirect mutagen 2- aminoanthracene. All extracts exhibited the highest ability to protect plasmid DNA against hydroxyl radicals induced DNA damages. The ethyl acetate (EA) and chloroform (Chl) extracts showed with high TEAC values radical of 0.95 and 0.81 mM respectively, against the ABTS^.+.</p> <p>Conclusion</p> <p>The present study revealed the antimutagenic and antioxidant potenty of plant extract from <it>Accacia salicina </it>leaves.</p

Phytochemical, antimicrobial, antioxidant and antigenotoxic potentials of Cyperus rotundus extracts

AbstractThe aqueous, ethyl acetate, methanolic and Total Oligomer Flavonoids (TOF) enriched extracts, obtained from the aerial parts of Cyperus rotundus, were investigated for their contents in phenolic compounds. Antioxidative activity using the NBT/riboflavin assay system, antimicrobial activity against Gram positive and Gram negative bacterial reference strains as well as antigenotoxic activity tested with the SOS chromotest assay were also studied. Significant antibacterial activity against reference strains; Staphylococcus aureus, Enterococcus faecalis, Salmonella enteritidis and Salmonella typhimurium, was detected in the presence of ethyl acetate and TOF enriched extracts. In addition to their antimicrobial activity, the same extracts showed a significant ability to inhibit nitroblue tetrazolium reduction by the superoxide radical in a non enzymatic O2.− generating system, and were also able to reduce significantly the genotoxicity induced by nifuroxazide and Aflatoxin B1. The antioxidant, antimicrobial and antigenotoxic activities exhibited by C. rotundus depend on the chemical composition of the tested extracts

Evaluation of in vitro antioxidant and apoptotic activities of Cyperus rotundus

AbstractObjectiveTo evaluate in vitro antioxidant and apoptotic activities of Cyperus rotundus (C. rotundus).MethodsThe phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C. rotundus aerial part were determined. In addition, these extracts were also investigated for their cytotoxic and apoptotic activities. The major compound of the methanol extract was isolated. Both methanol and aqueous extracts (300, 150, and 50 μg/mL) were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay system. However, 16, 8, and 4 mg/mL of each extract were tested to investigate their OH. formation scavenging potential. Aqueous extract (800, 400, and 200 μg/mL) and methanol extract (350, 175, and 88 μg/mL) were tested against lipid peroxidation, induced by 75 μM H2O2. The cytotoxicity (by MTT assay) and cell DNA fragmentation of both extracts were evaluated towards K562 and L1210 cell lines. The major compound was obtained from the butanol fraction of methanol extract and its structure was determined by RMN spectroscopic analysis.ResultsThe methanol and aqueous extracts showed respectively, 88% and 19% inhibition of xanthine oxidase activity. Yet, the same extracts inhibited lipid peroxidation by 61.5% and 42.0%, respectively. Both extracts inhibited OH. formation by 27.1% and 25.3%, respectively. Only methanol extract induced DNA degradation. Orientin was determined as the major compound isolated from the butanol fraction of methanol extract.ConclusionsIt appears that C. rotundus extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer

Inhibitory effect of Teucrium ramosissimum extracts on aflatoxin B1, benzo[a]pyrene, 4-nitro-o-phenylenediamine and sodium azide induced mutagenicity: Correlation with antioxidant activity

AbstractThe mutagenic potential of total oligomers flavonoids (TOF), ethyl acetate (EA) and petroleum ether (PE) extracts from aerial parts of Teucrium ramosissimum was assessed using Ames Salmonella tester strains TA98, TA100 and TA1535 with and without metabolic activation (S9). None of the different extracts produced a mutagenic effect. Likewise, the antimutagenicity of the same extracts was tested using the “Ames test”. Our results showed that T. ramosissimum extracts possess antimutagenic activity against all the tested genotoxicants (aflatoxin B1, benzo[a]pyrene, 4-nitro-o-phenylenediamine and sodium azide) in the Salmonella assay systems used in this study. In addition, all extracts showed important free radical scavenging activity toward the radicals DPPH and ABTS except the PE extract