Study on breeding of imported brood stocks Litopenaeus vannamei in Iran environment

Regarding to breeding the Litopenaeus vannamei, a total of 126 pairs of broodstocks were imported from Hawaii to Iran in 2004 and 2005, and then transferred to the Bandargah Research Station in Bushehr. The female broodstocks were ablated, and were feeded 3 times per day with cuttlefish, small size shrimp and Nereis worm with a ratio of 30% body weight. The water exchange were done 3 times per day. During the years 2004 and 2005 a total 1700000 naupli were produced of which 772000 specimens of pl13 and pl7 were harvested and then transferred to Helleh Site for carrying out the next culture project. The average naupli and postlarvae were 170000 and 92000 in proportion to each broodstock. Also the mean survival rate was estimated 54%

Site-specific differences in osteoblast phenotype, mechanical loading response and estrogen receptor-related gene expression

King’s Health Partner’s Research and Development Challenge Fun

Study on brood stock management of Litopenaeus vannamei using imported post larvae

In order to domesticate Litopenaeus vannamei as a solution to single species shrimp aquaculture of Penaeus indicus and to reduce risk of shrimp culture in emergency cases, this study was carried out. Initially the postlarvae reared from imported L. vannamei were cultured. After culture season the adult shrimps were transported to greenhouse ponds for wintering. It is then relocated to hatchery saloon. After eye stalk ablation and maturation the female brood stocks were introduced to matured male for copulation at ponds. During the study period, male and female shrimp’s attained full maturation at end – April by temperature over 25ºC, the copulation process was started significantly. Finally of the 12 instances of copulation, one instance resulted nauplii production. The best maturation was observed at 28 ֯C ±1 and salinity30 to 33PPT and pH of 8 to 8.3 Fresh sea worms and squid showed the highest effect on fecundity in brood stocks. Besides, the spring season was obtained to be the best period for brood stock production in Bushehr province

Investigation of two crop culture of western white shrimp

Two crop culture of western white shrimp with 2 treatments and 2 replicates in each treatments in earthen ponds with area 0.4 hectares at a density of 20 pl 18 in each square meter were done. For the culture of shrimp in the second stage, the post larvae cultured in nursery pond with area of 0.25 hectare, and density of 96 post larvae in each square meter, and then transfer to the rearing ponds. In each pond, 2 aerators with potency of 3 horsepower fixed. Every day horsepower clarified at 21p.m. to 23 p.m and out at 6 a.m to 7 a.m. The first stage of culture begun in 19 April with pl 18. The yield pick-up after 96 days. The stocking of pl in the nursery ponds was done in 25 July with pl16 and after 38 days hunted and transferred to second stage culture. The second culture was done in 1 September and pick-up after 65 days. For feeding of shrimps we used commercial food numbers 4001 until 4006. In the first mount the feeding of shrimps were done as blind feed and then with the shrimps biomass and with research of feeding trays. Water temperature, dissolved oxygen and pH daily in 2 times at the morning (6 a.m) and evening (at 15 p.m) and the salinity, transparency and water depth 1 time daily measured. In the first culture period (96 days), the mean weight, length, daily weight gain, FCR, survival percent shrimp production were 14.03 gr, 12.08 cm, 0.15 gr, 1.02 gr, 92 percent and 2132 kilograms and in the second culture period (65 days) 14.55 gr, 12.48 cm, 0.22 gr, 0.84, 73 percent and 2675 kilogarms measuered respectively. Totally, in the second period culture the initial weight of shrimps was higher than the first period culture 1.95 gr and 0.008 gr respectively, therefore growth indexes in the second period were better than the first culture period. Coefficient of determination of weight and lenth of shrimps with increase of cultue days, in the first and second culture periods, in the total cases the number were higher than 0.97. This shown that the role of increase of days on the increase of weight and lenth is higher than 97 percent. Also, determination of product moment, showed that there is very strong correlation between increase of days and increase of weight and lenth and between weight and lenth (0.97 to 0.99)

Study on effect of enriched Artemia with (HUFA) on reproductive parameters and comparing with current method (used marine worm) in Litopenaeus vannamei

Nowadays, the use of live food enrichment is very common in aquaculture and as a practical method introduced. Artemia a potential food source and quality is suitable, it will have used for feeding fish and crustaceans (shrimp). In this study, Artemia enriched with unsaturated fatty acids used for increase the sexual maturity of the white leg shrimp (Litopenaeus vannamei). Thus, the young brood stocks transferred to Bandarga Research station. In order to achieve optimal weight, Brood stocks were feeding by food concentrate and food frozen fresh. In this study such as 3 treatments each with 3 replicates, which brood stocks were stocking in the fiberglass thank with density 2 to 3 (pieces/m^2). They were feeding with meals different including 1) squid , pen shale and marine polychaeta worm (Perinereis nuntia), 2) squid , pen shale and Artemia biomass "fransiscan", 3) squid , pen shale and Artemia biomass enriched with unsaturated fatty acid (HUFA) SPARI SELCO solution after were eye ablation. The results showed that sexual maturity increased in the brood stocks fed with Artemia enriched which with significantly higher brood stocks fed with Artemia biomass and the marine worm (P 0.05). The other hand, histological studies showed that brood stocks fed with Artemia enriched than other two treatments contain were many mature follicles ready for ovulation. However, there was more absolute fecundity in the brood stocks fed with Artemia enriched, but don't found any significant differences in competition with other two treatments (P>0.05). In addition, during this period, measured and recorded all factors physicochemical water and environment daily. Following this study showed that if the Artemia enriched as part of the diet can both caused eliminate unsaturated fatty acid deficiency diet and improved ovarian development and increase sexual maturity brood stocks female in a short interval. Finally, the results analyzed with statistical software spss 9.0, one way ANOVA and Tukey test

Lack of effect of adenosine on the function of rodent osteoblasts and osteoclasts in vitro

Extracellular ATP, signalling through P2 receptors, exerts well-documented effects on bone cells, inhibiting mineral deposition by osteoblasts and stimulating the formation and resorptive activity of osteoclasts. The aims of this study were to determine the potential osteotropic effects of adenosine, the hydrolysis product of ATP, on primary bone cells in vitro. We determined the effect of exogenous adenosine on (1) the growth, alkaline phosphatase (TNAP) activity and bone-forming ability of osteoblasts derived from the calvariae of neonatal rats and mice and the marrow of juvenile rats and (2) the formation and resorptive activity of osteoclasts from juvenile mouse marrow. Reverse transcription polymerase chain reaction (RT-PCR) analysis showed marked differences in the expression of P1 receptors in osteoblasts from different sources. Whilst mRNA for the A1 and A2B receptors was expressed by all primary osteoblasts, A2A receptor expression was limited to rat bone marrow and mouse calvarial osteoblasts and the A3 receptor to rat bone marrow osteoblasts. We found that adenosine had no detectable effects on cell growth, TNAP activity or bone formation by rodent osteoblasts in vitro. The analogue 2-chloroadenosine, which is hydrolysed more slowly than adenosine, had no effects on rat or mouse calvarial osteoblasts but increased TNAP activity and bone formation by rat bone marrow osteoblasts by 30–50 % at a concentration of 1 μM. Osteoclasts were found to express the A2A, A2B and A3 receptors; however, neither adenosine (≤100 μM) nor 2-chloroadenosine (≤10 μM) had any effect on the formation or resorptive activity of mouse osteoclasts in vitro. These results suggest that adenosine, unlike ATP, is not a major signalling molecule in the bone

Derivation of lowland riparian wetland deposit architecture using geophysical image analysis and interface detection

For groundwater-surface water interactions to be understood in complex wetland settings, the architecture of the underlying deposits requires investigation at a spatial resolution sufficient to characterize significant hydraulic pathways. Discrete intrusive sampling using conventional approaches provides insufficient sample density and can be difficult to deploy on soft ground. Here a noninvasive geophysical imaging approach combining three-dimensional electrical resistivity tomography (ERT) and the novel application of gradient and isosurface-based edge detectors is considered as a means of illuminating wetland deposit architecture. The performance of three edge detectors were compared and evaluated against ground truth data, using a lowland riparian wetland demonstration site. Isosurface-based methods correlated well with intrusive data and were useful for defining the geometries of key geological interfaces (i.e., peat/gravels and gravels/Chalk). The use of gradient detectors approach was unsuccessful, indicating that the assumption that the steepest resistivity gradient coincides with the associated geological interface can be incorrect. These findings are relevant to the application of this approach in settings with a broadly layered geology with strata of contrasting resistivities. In addition, ERT revealed substantial structures in the gravels related to the depositional environment (i.e., braided fluvial system) and a complex distribution of low-permeability putty Chalk at the bedrock surface—with implications for preferential flow and variable exchange between river and groundwater systems. These results demonstrate that a combined approach using ERT and edge detectors can provide valuable information to support targeted monitoring and inform hydrological modeling of wetlands

Effects of thermal water inhalation in chronic upper respiratory tract infections in elderly and young patients

Background: Chronic upper respiratory tract infections (cURTI) are very frequent illnesses which occur at any age of life. In elderly, cURTI are complicated by immunosenescence, with involvement of lung immune responsiveness. Results: In the present study, 51 elderly (age range: 66-86) and 51 young (age range 24-58) cURTI patients underwent a single cycle (two weeks) of inhalatory therapy with salt-bromide-iodine thermal water in the thermal station "Margherita di Savoia" (Margherita di Savoia, BAT, Italy). Peripheral blood serum cytokines and clinical assessment were performed before therapy (T0) and after six months (T1) and 12 months (T2) from inhalatory treatment. In both elderly and young patients, at baseline an increased release of T helper (h)1-related cytokines [interleukin (IL)-2 and interferon-γ] and of Th2-related cytokine (IL-4) was documented. Inhalatory treatment reduced the excessive secretion of all the above-cited cytokines. IL-10 values were above normality at all times considered in both groups of patients. In addition, an increase in IL-17 and IL-21 serum levels following therapy was observed in both groups of patients. Pro-inflammatory cytokine (IL-1β, IL-6, IL-8 and tumor necrosis factor-α) baseline values were lower than normal values at T0 in both elderly and young cURTI patients. Their levels increased following inhalatory treatment. Clinically, at T2 a dramatic reduction of frequency of upper respiratory tract infections was recorded in both groups of patients. Conclusion: Thermal water inhalation is able to modulate systemic immune response in elderly and young cURTI patients, thus reducing excessive production of Th1 and Th2-related cytokines, on the one hand. On the other hand, increased levels of IL-21 (an inducer of Th17 cells) and of IL-17 may be interpreted as a protective mechanism, which likely leads to neutrophil recruitment in cURTI patients. Also restoration of pro-inflammatory cytokine release following inhalatory therapy may result in microbe eradication. Quite importantly, the maintenance of high levels of IL-10 during the follow-up would suggest a consistent regulatory role of this cytokine in attenuating the pro-inflammatory arm of the immune response