Acquiring high-resolution wind measurements by modifying radiosonde sounding procedures

High-resolution wind measurements are crucial for the understanding of dynamic processes in the atmosphere. In the troposphere and lower stratosphere, radiosondes provide a good vertical resolution of a few metres, but the wind data are usually low-pass filtered by the manufacturer in order to suppress disturbances in the data caused by the spurious motions of the sonde. As an example, the filter within the standard processing of Vaisala radiosondes becomes effective at vertical scales below 300 m for an ascent rate of 5 m s−1. We describe a method for increasing the usable resolution of radiosonde wind measurements. The main ideas are to avoid self-induced motions of the balloon by keeping it in the subcritical Reynolds number range, to avoid typical pendulum motions of a 15 s period by using a shorter string, and to use data from a descending balloon in order to avoid disturbances from the wake of the balloon affecting temperature and humidity measurements due to the decreased string length. We demonstrate that our changes in hardware and software allow for artefact-free wind data down to scales of 50 m, while the remaining disturbances on even smaller scales are removed. Accordingly, the usable resolution of the wind data has been increased by a factor of 6, compared to the standard data output, at relatively low cost.</p

Chemical Engineering Division waste management programs. Quarterly report, January--March 1975

Development work on the study of consolidation techniques for Zircaloy fuel-cladding hulls included scouting tests on volatility schemes for separating the zirconium as the volatile tetrachloride and ignition tests on several Zircaloy materials to further characterize the pyrophoric behavior of Zircaloy. All tests were with nonirradiated metal pending acquisition of irradiated samples. Installation is nearly complete of a glovebox facility for studies on the salvage of alpha-contaminated metals by pyrochemical methods. Disposal of a major portion of fission product tritium formed in light water reactor fuels by deep- well injection of the low-level aqueous waste from plants reprocessing such fuels is being evaluated. The question of siting is a very important factor in determining the feasibility of this particular disposal option. A review is given of the status of information for U. S. sedimentary basins, the areas most likely to be generally suitable for siting of waste wells. Work on the reliability of high-level-waste canisters included an examination of creep, shot- peening, and subcooling of the filler canister below storage temperatures, as methods of relieving stresses induced in canisters due to differential contraction of canister and glass during cooling. A method was investigated for relieving stresses in calcine-filled canisters. Properties of fission product oxides were examined to elucidate possible adverse corrosive effects at the canister-waste interface. (LK

Conversion of descriptor representations to state-space form: an extension of the shuffle algorithm

This paper proposes a systematic procedure for the determination of state-space models from an available descriptor representation of a linear dynamic system. The goal is to determine a state equation, a set of algebraic equations and an output equation in terms of the state and input variables. It is shown that standard methods may fail to convert the descriptor representation to state-space form, even for simple electrical circuit models obtained from Kirchoff’s laws and constitutive element equations. A novel procedure to address this problem is then proposed as an extension of the classic shuffle algorithm combined with a singular value decomposition approach. In addition to an illustrative example involving a simple electrical circuit, the proposed method is employed in a case study involving the modeling of three-dimensional RLC networks with a large number of components

Socio-economic vision graph generation and handover in distributed smart camera networks

In this article we present an approach to object tracking handover in a network of smart cameras, based on self-interested autonomous agents, which exchange responsibility for tracking objects in a market mechanism, in order to maximise their own utility. A novel ant-colony inspired mechanism is used to learn the vision graph, that is, the camera neighbourhood relations, during runtime, which may then be used to optimise communication between cameras. The key benefits of our completely decentralised approach are on the one hand generating the vision graph online, enabling efficient deployment in unknown scenarios and camera network topologies, and on the other hand relying only on local information, increasing the robustness of the system. Since our market-based approach does not rely on a priori topology information, the need for any multicamera calibration can be avoided. We have evaluated our approach both in a simulation study and in network of real distributed smart cameras

The Doppler wind, temperature, and aerosol RMR lidar system at Kühlungsborn, Germany – Part 1: Technical specifications and capabilities

This paper describes the technical specifications of the extensions made to the middle-atmospheric lidar facility at the Leibniz Institute of Atmospheric Physics in Kühlungsborn, Germany (54.12° N, 11.77° E). The upgrade complements the existing daylight-capable Rayleigh–Mie–Raman (RMR) temperature lidar with a nighttime-only RMR wind–temperature lidar. The new system comprises an independent lidar with laser, telescopes, and detectors, which is synchronized with and adapted to the (old) temperature lidar. As a result, with the combination of RMR lidars the atmosphere is probed with three (vertical and tilted) beams. This work intends to highlight the recent innovations in the construction of a Doppler–Rayleigh lidar system using the single-edge iodine-cell technique, which allows for the simultaneous measurement of wind, temperature, and aerosols. We will detail supporting subsystems that allow for a high degree of lidar automation and concisely provide key technical information about the system that will support readers in the development of additional RMR wind–temperature lidar systems. We show an example of time-resolved temperature and wind soundings reaching up to ∼ 90 km. These data agree well with ECMWF-IFS profiles between 35 and ∼ 50 km but show a much larger variability above. In the companion article, we will present the algorithm design and uncertainty budgets associated with the data processing chain.</p

High-throughput, quantitative analyses of genetic interactions in E. coli.

Large-scale genetic interaction studies provide the basis for defining gene function and pathway architecture. Recent advances in the ability to generate double mutants en masse in Saccharomyces cerevisiae have dramatically accelerated the acquisition of genetic interaction information and the biological inferences that follow. Here we describe a method based on F factor-driven conjugation, which allows for high-throughput generation of double mutants in Escherichia coli. This method, termed genetic interaction analysis technology for E. coli (GIANT-coli), permits us to systematically generate and array double-mutant cells on solid media in high-density arrays. We show that colony size provides a robust and quantitative output of cellular fitness and that GIANT-coli can recapitulate known synthetic interactions and identify previously unidentified negative (synthetic sickness or lethality) and positive (suppressive or epistatic) relationships. Finally, we describe a complementary strategy for genome-wide suppressor-mutant identification. Together, these methods permit rapid, large-scale genetic interaction studies in E. coli

Effects of an early life diet containing large phospholipid-coated lipid globules on hepatic lipid metabolism in mice

We recently reported that feeding mice in their early life a diet containing a lipid structure more similar to human milk (eIMF, Nuturis) results in lower body weights and fat mass gain upon high fat feeding in later life, compared to control (cIMF). To understand the underlying mechanisms, we now explored parameters possibly involved in this long-term effect. Male C57BL/6JOlaHsd mice, fed rodent diets containing eIMF or cIMF from postnatal (PN) day 16–42, were sacrificed at PN42. Hepatic proteins were measured using targeted proteomics. Lipids were assessed by LC–MS/MS (acylcarnitines) and GC-FID (fatty-acyl chain profiles). Early life growth and body composition, cytokines, and parameters of bile acid metabolism were similar between the groups. Hepatic concentrations of multiple proteins involved in β-oxidation (+ 17%) the TCA cycle (+ 15%) and mitochondrial antioxidative proteins (+ 28%) were significantly higher in eIMF versus cIMF-fed mice (p < 0.05). Hepatic l-carnitine levels, required for fatty acid uptake into the mitochondria, were higher (+ 33%, p < 0.01) in eIMF-fed mice. The present study indicates that eIMF-fed mice have higher hepatic levels of proteins involved in fatty acid metabolism and oxidation. We speculate that eIMF feeding programs the metabolic handling of dietary lipids

Inhibition of the succinyl dehydrogenase complex in acute myeloid leukemia leads to a lactate-fuelled respiratory metabolic vulnerability

Metabolic programs can differ substantially across genetically distinct subtypes of acute myeloid leukemia (AML). These programs are not static entities but can change swiftly as a consequence of extracellular changes or in response to pathway-inhibiting drugs. Here, we uncover that AML patients with FLT3 internal tandem duplications (FLT3-ITD+) are characterized by a high expression of succinate-CoA ligases and high activity of mitochondrial electron transport chain (ETC) complex II, thereby driving high mitochondrial respiration activity linked to the Krebs cycle. While inhibition of ETC complex II enhances apoptosis in FLT3-ITD+ AML, cells also quickly adapt by importing lactate from the extracellular microenvironment. 13C3-labelled lactate metabolic flux analyses reveal that AML cells use lactate as a fuel for mitochondrial respiration. Inhibition of lactate transport by blocking Monocarboxylic Acid Transporter 1 (MCT1) strongly enhances sensitivity to ETC complex II inhibition in vitro as well as in vivo. Our study highlights a metabolic adaptability of cancer cells that can be exploited therapeutically.</p