48 research outputs found

    Partial characterization of the lipidome of the cold-water scallop, Chlamys islandica

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    Fingerprinting of the main lipid components of the digestive gland of the Icelandic scallop—Chlamys islandica—has been performed by ultra-high-performance liquid chromatography coupled with time of flight high-resolution mass spectrometry, UHPLC-HRMS/ToF. This method allowed the identification of 224 lipids, including phosphatidylcholines (PC), plasmanyl (PC-O)/plasmenyl (PC-P) phosphatidylcholines, lyso-phosphatidylcholines (LPC), and their plasmanyl/plasmenyl forms (LPC-O/LPC-P). Diacylglycerols (DG), triacylglycerols (TG), and cholesteryl esters (CE) were the neutral lipids (NL) analyzed. While all of the lipids showed a strong seasonal dependence in terms of quantity, only NLs presented significant qualitative changes. Principal component analysis (PCA) of TG and DG profiles evidenced a prevalence of low unsaturated TGs and DGs in spring, which were replaced by species with a higher degree of unsaturations in summer. In autumn, long and highly unsaturated TGs constitute the lipid fraction of the digestive gland of the scallop, while DG species offer a mixed profile. This study contributes to the characterization and the elucidation of the lipidome of Chlamys islandica and provides baseline data for further study of the effects of pollutants on the lipidome of the Icelandic scallop, often used as a sentinel species in biomonitoring programs

    Evidence of altered fertility in female roach (Rutilus rutilus) from the River Seine (France)

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    A large variety of anthropogenic chemicals present in the aquatic environment have been shown to be able to alter the endocrine system of exposed organisms, potentially impacting their reproductive function. The aim of this study was to assess the effects of environmental pollution on the reproductive system of wild female roach (Rutilus rutilus) from the Seine River (Normandy, France). A suite of biomarkers of endocrine disruption including gonado-somatic index, plasmatic vitellogenin, gonadal aromatase activity and histological parameters (oocyte diameter and gonad maturation) were studied. Female fish from the polluted sites showed a number of reproductive alterations, including inhibited gonad maturation, reduced oocyte growth, reduced levels of plasmatic vitellogenin and 3-fold lower gonadal aromatase activity than females collected in the reference site. Overall, these results highlight the presence of endocrine disruption in female roach from the Seine River. © 2014 Elsevier Ltd. All rights reserved
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