Swain Committee Report

Letter addressed to the Secretary of the Navy, the Honorable Josephus H. Daniels, from the Committee appointed by the President of the Society for the Promotion of Engineering Education, to visit the U.S. Naval Academy in Annapolis, MD. The purpose was to evaluate the work of the Post Graduate School. The committee recommended enlarging the enrollment of the Post Graduate School and providing for appropriate funding for buildings, equipment and curricula

Structure of the master regulator Rns reveals an inhibitor of enterotoxigenic Escherichia coli virulence regulons

Enteric infections caused by the gram-negative bacteria enterotoxigenic Escherichia coli (ETEC), Vibrio cholerae, Shigella flexneri, and Salmonella enterica are among the most common and affect billions of people each year. These bacteria control expression of virulence factors using a network of transcriptional regulators, some of which are modulated by small molecules as has been shown for ToxT, an AraC family member from V. cholerae. In ETEC the expression of many types of adhesive pili is dependent upon the AraC family member Rns. We present here the 3 Å crystal structure of Rns and show it closely resembles ToxT. Rns crystallized as a dimer via an interface similar to that observed in other dimeric AraC’s. Furthermore, the structure of Rns revealed the presence of a ligand, decanoic acid, that inhibits its activity in a manner similar to the fatty acid mediated inhibition observed for ToxT and the S. enterica homologue HilD. Together, these results support our hypothesis that fatty acids regulate virulence controlling AraC family members in a common manner across a number of enteric pathogens. Furthermore, for the first time this work identifies a small molecule capable of inhibiting the ETEC Rns regulon, providing a basis for development of therapeutics against this deadly human pathogen

Flamingo Vol. II N 3

Punch Bowl. A Previous Engagement . Prose. 1. Burr. Untitled. Prose. 1. Ubersax, Delmar. Untitled. Picture. 4. Mather, William G. Shelf 378C . Prose. 5. Bennett, G.W. Esotery . Poem. 6. W.A.V. Untitled. Poem. 6. Anonymous. Approved Subjects of Conversation . Poem. 7. Anonymous. Pipe Up . Prose. 7. Anonymous. Denison\u27s Hall of Fame: Francis W. Shepardson . Prose. 8 M.E. Percy . Poem. 9.; A.F.T. Triolet . Poem. 9. Anonymous. Untitled. Picture. 9. W.G.K. The Message of a Leader-Autobiographical . Prose. 9. Keeler, Clyde. Untitled. Picture. 10. Anonymous. Untitled. Prose. 10. Potter, W.M. Prose Fiction . Poem. 11. Keeler, Clyde. Untitled. Picture. 11. Anonymous. The Night After Christmas . Prose. 11. Anonymous. Untitled. Prose. 11. Anonymous. Popular Illusions Shattered . Prose. 11. Anonymous. Throw Him Into The Green River! . Poem. 12. Anonymous. Candid . Prose. 12. Holt, Kilburn. Favorite Poems (Revised Edition) . Poem. 12. Anonymous. Untitled. Prose. 14. Anonymous. Proof that Winter is Here . Cartoon. 16. F. The Inefficiency Medal . Prose. 18. Anonymous. Untitled. Picture. 18. Anonymous. Untitled. Prose. 18. Ed. A Pathetic Fallacy . Poem. 19. Ubersax, Delmar. Untitled. Picture. 19. Anonymous. Untitled. Prose. 19. Anonymous. Untitled. Picture. 19. Oh Min. The Baliff and The Bunk . Prose. 20. Anonymous. Reservoir . Prose. 20. Anonymous. Untitled. Prose. 20. Ed. and Delmar Ubersax. A Lass and a Lack . Picture. 20. Anonymous. Thrice Told Tales . Prose. 21. Anonymous. Untitled. Prose. 21. Anonymous. Untitled. Prose. 22. Keeler, Clyde. Untitled. Picture. 22. R. The Lover Sings . Poem. 23. Anonymous. Untitled. Prose. 23. Anonymous. Untitled. Picture. 23. Octopus. Untitled. Prose. 23. Anonymous. Untitled. Picture. 24. Anonymous. Untitled. Prose. 24. Keeler, Clyde. Untitled. Picture. 25. Anonymous. Untitled. Prose. 25. Punch Bowl. Untitled. Prose. 26. Orange Peel. Untitled. Prose. 26. Lampoon. Untitled. Prose. 26. Octopus. Untitled. Prose. 26. Sun Dodger. Untitled. Prose. 27. Phoenix. Untitled. Prose. 27. Dirge. Untitled. Prose. 27. Lampoon. Untitled. Prose. 27. Siren. Untitled. Prose. 27. Jester. Untitled. prose. 29. Frivol. Untitled. Prose. 29. Voo-Doo. Untitled. Prose. 29. Lampoon. Untitled. Prose. 29. Royal Gaboon. Untitled. Prose. 30. Octopus. Untitled. Prose. 30. Anonymous. Page Mr. Volstead . Prose. 30. Chaparral. Missing Cylinder . Prose. 30. Frivol. Untitled. Prose. 31. Widow. Bubbling over . Prose. 31. Juggler. The Order of The Bawth . Prose. 31. Tiger. Untitled. Prose. 31

Cardiopulmonary phenotype associated with human PHD2 mutation.

Oxygen-dependent regulation of the erythropoietin gene is mediated by the hypoxia-inducible factor (HIF) family of transcription factors. When oxygen is plentiful, HIF undergoes hydroxylation by a family of oxygen-dependent prolyl hydroxylase domain (PHD) proteins, promoting its association with the von Hippel-Lindau (VHL) ubiquitin E3 ligase and subsequent proteosomal degradation. When oxygen is scarce, the PHD enzymes are inactivated, leading to HIF accumulation and upregulation not only of erythropoietin expression, but also the expression of hundreds of other genes, including those coordinating cardiovascular and ventilatory adaptation to hypoxia. Nevertheless, despite the identification of over 50 mutations in the PHD-HIF-VHL pathway in patients with previously unexplained congenital erythrocytosis, there are very few reports of associated cardiopulmonary abnormalities. We now report exaggerated pulmonary vascular and ventilatory responses to acute hypoxia in a 35-year-old man with erythrocytosis secondary to heterozygous mutation in PHD2, the most abundant of the PHD isoforms. We compare this phenotype with that reported in patients with the archetypal disorder of cellular oxygen sensing, Chuvash polycythemia, and discuss the possible clinical implications of our findings, particularly in the light of the emerging role for small molecule PHD inhibitors in clinical practice

Two independent S-phase checkpoints regulate appressorium-mediated plant infection by the rice blast fungus Magnaporthe oryzae

To cause rice blast disease, the fungal pathogen Magnaporthe oryzae develops a specialized infection structure called an appressorium. This dome-shaped, melanin-pigmented cell generates enormous turgor and applies physical force to rupture the rice leaf cuticle using a rigid penetration peg. Appressorium-mediated infection requires septin-dependent reorientation of the F-actin cytoskeleton at the base of the infection cell, which organizes polarity determinants necessary for plant cell invasion. Here, we show that plant infection by M. oryzae requires two independent S-phase cell-cycle checkpoints. Initial formation of appressoria on the rice leaf surface requires an S-phase checkpoint that acts through the DNA damage response (DDR) pathway, involving the Cds1 kinase. By contrast, appressorium repolarization involves a novel, DDR-independent S-phase checkpoint, triggered by appressorium turgor generation and melanization. This second checkpoint specifically regulates septin- dependent, NADPH oxidase-regulated F-actin dynamics to organize the appressorium pore and facilitate entry of the fungus into host tissue

Compilation of analyses of risks and measures, deliverable 8.2 of the H2020 project SafetyCube

This deliverable provides information on how the information on road safety risks and measures that has been collected within SafetyCube, is processed, stored and made available to users through the SafetyCube Decision Support System (DSS) [...continues]

Analysis of the stakeholder survey: perceived priority and availability of data and tools and relation to the stakeholders' characteristics. Deliverable 1.5 (Vol. 1) of the EC FP7 project DaCoTA

Analysis of the stakeholder survey: perceived priority and availability of data and tools and relation to the stakeholders' characteristics. Deliverable 1.5 (Vol. 1) of the EC FP7 project DaCoT

i-DREAMS: an intelligent driver and road environment assessment and monitoring system

The objective of the Horizon2020 project i-DREAMS is to setup a framework for the definition, development and validation of a context-aware ‘safety tolerance zone’. Taking into account, on the one hand, driver-related background factors and real-time risk-related physiological indicators, and on the other hand, driving task-related complexity indicators a real-time assessment will be made to determine if a driver is within acceptable boundaries of safe operation. Additionally, interventions will be developed to prevent drivers from getting too close to the boundaries of unsafe operation. These will be composed of in-vehicle interventions, and interventions aimed at enhancing the knowledge, attitudes and behavioural reaction of drivers. A holistic approach will be taken suitable for use in multiple transport modes. Initial testing will take place in a driving simulator after which promising interventions will be tested and validated under real-world conditions in a testbed of 600 drivers across 5 EU countries

The future decision support system, deliverable 8.5 of the H2020 project SafetyCube

The European Road Safety Decision Support System (DSS) is a comprehensive “one stop shop” designed to inform evidence based policy by providing state of the art scientific knowledge on road safety. A short promotion video is available here: www.youtube.com/watch?v=Y-mVUde3knU. The DSS (www.roadsafety-dss.eu) has a user friendly web-based interface allowing users access to compressive information about a large range of road safety risk (problems) and measures (solutions), and links between the two. In addition, users are presented with information about serious road injuries, accident scenario fact sheets and an Economic Efficiency Evaluation (E3) tool. The E3 tool allows users to evaluate the cost effectiveness of road safety measures as well as providing a selection of worked examples. The European Road Safety DSS was developed by the European Commission supported Horizon 2020 project Safety CaUsation, Benefits and Efficiency (SafetyCube). The object of SafetyCube was to develop an innovative road safety Decision Support System (DSS) that will enable policy-makers and stakeholders to select and implement the most appropriate strategies, measures and cost-effective approaches to reduce casualties of all road user types and all severities. Detailed information about the development and DSS status at the end of the SafetyCube project are available in Yannis & Papadimitriou (2018). An overview of the DSS scientific content and a summary of the methodology used to develop the DSS can be found in the SafetyCube Final Project Report (Thomas & Talbot, 2018). The present Deliverable (8.5) gives a brief overview of the current state of the art DSS, describes the future enhanced version of the DSS and provides information for potential funder(s). Opportunity is available for new funders to support the European road safety DSS as it is developed and enhanced for future users. Through supporting the DSS, the future funder(s) will be contributing the Global UN Sustainable Development Goals on road safety by taking a leading position to actively promote effective solutions to road safety’s most pressing challenges. Aspirations for the future DSS will make the scientific content more accessible through translation of content in to local languages and filtering information into the manner most appropriate for low and middle income countries. The content will be expanded to include more topics and more detail about existing topics. Expansion of knowledge will include knowledge about implementing measures and a focus on the interdependences of road safety measures considering the impacts of implementing measures in combination. In addition to the future visions for content there are also aspirations for the future web based interface. A key enhancement will be to give users the ability to customise the display and select the information they would like to see for each individual coded study. The best case future for DSS operation is that of extended growth supported by considerable external funding. The exact structure, legal entity and governance of the future enhanced DSS will be decided in collaboration between the SafetyCube consortia and the future funder(s). In this cooperative way funding partners will have the chance to influence the development process in the manner most appropriate to meet their stakeholder needs. It is envisaged that the future DSS will be financed by several Organisations, therefore, the governance, time schedule and strategy for extended growth will be mutually decided. Within the SafetyCube project activities have been undertaken to advertise the DSS and provide information for potential funders. The European road safety DSS is the first integrated road safety support system developed in Europe. It aims to be the “go to tool”for road safety knowledge. The next funder(s) of the DSS have the exciting opportunity to take the DSS to the next level in facilitating the future of evidence based road safety policy making, ensuring safe roads for all