Attitudes of boys and girls toward some common forms of behavior

Not available.George Spencer SchwartzNot ListedNot ListedMaster of ScienceDepartment Not ListedCunningham Memorial Library, Terre Haute, Indiana State University.isua-thesis-1950-schwartzMastersTitle from document title page. Document formatted into pages: contains 108p. : ill. Includes appendix and bibliography

On the proof-theory of a first-order extension of GL

We introduce a first order extension of GL, called ML3, and develop its proof theory via a proxy cut-free sequent calculus GLTS. We prove the highly nontrivial result that cut is a derived rule in GLTS, a result that is unavailable in other known first-order extensions of GL. This leads to proofs of weak reflection and the related conservation result for ML3, as well as proofs for Craig’s interpolation theorem for GLTS. Turning to semantics we prove that ML3 is sound with respect to arithmetical interpretations and that it is also sound and complete with respect to converse well-founded and transitive finite Kripke models. This leads us to expect that a Solovay-like proof of arithmetical completeness of ML3 is possible

Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli

Georgios Skretas, Sean Carroll, and George Georgiou are with the Department of Chemical Engineering, University of Texas at Austin, Austin, TX 78712, USA -- George Georgiou is with the Department of Biomedical Engineering, University of Texas at Austin and the Section of Microbiology and Molecular Genetics, University of Texas at Austin, Austin, TX 78712, USA -- Georgios Skretas and George Georgiou are with the Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA -- Shawn DeFrees, Karl F. Johnson, and Marc F. Schwartz are with Neose Technologies Inc, 102 Rock Road, Horsham, PA, 19044, USABackground: The presence of terminal, surface-exposed sialic acid moieties can greatly enhance the in vivo half-life of glycosylated biopharmaceuticals and improve their therapeutic efficacy. Complete and homogeneous sialylation of glycoproteins can be efficiently performed enzymically in vitro but this process requires large amounts of catalytically active sialyltransferases. Furthermore, standard microbial hosts used for large-scale production of recombinant enzymes can only produce small quantities of glycosyltransferases of animal origin, which lack catalytic activity. Results and conclusion: In this work, we have expressed the human sialyltransferase ST6GalNAc I (ST6), an enzyme that sialylates O-linked glycoproteins, in Escherichia coli cells. We observed that wild-type bacterial cells are able to produce only very small amounts of soluble ST6 enzyme. We have found, however, that engineered bacterial strains which possess certain types of oxidative cytoplasm or which co-express the molecular chaperones/co-chaperones trigger factor, DnaK/DnaJ, GroEL/GroES, and Skp, can produce greatly enhanced amounts of soluble ST6. Furthermore, we have developed a novel high-throughput assay for the detection of sialyltransferase activity and used it to demonstrate that the bacterially expressed ST6 enzyme is active and able to transfer sialic acid onto a desialylated O-glycoprotein, bovine submaxillary mucin. To the best of our knowledge, this is the first example of expression of active human sialyltransferase in bacteria. This system may be used as a starting point for the evolution of sialyltransferases with better expression characteristics or altered donor/acceptor specificities.Chemical EngineeringBiomedical EngineeringInstitute for Cellular and Molecular [email protected]

‘Off with their heads’ won’t make ABQ safer

Frustrated by crime in Albuquerque and the time it will require to address it rationally, and, perhaps, spurred on by bail bondsmen with their own financial interests, some in Albuquerque seem to prefer the rantings of the Queen of Hearts in Alice in Wonderland to our Constitutional law

2004-2005 Conservatory on Parade

https://spiral.lynn.edu/conservatory_philharmonia/1056/thumbnail.jp

2003-2004 Symphonic Knights Series No. 3

Program Overture from Ruslan and Ludmilla / Mikhail Ivanovich Glinka Violin Concerto in D Minor, op. 47 / Jean Sibelius Sergiu Schwartz, violin Overture from Don Giovanni / Wolfgang Amadeus Mozart Don Juan, op. 20 / Richard Strausshttps://spiral.lynn.edu/conservatory_philharmonia/1038/thumbnail.jp