Recent topics of pharmacology in oriental medicine : detoxification of morphine addiction in the mouse narcotic dependence model using extracts from psidium guajava leaves

The effects of a polar fraction of an extract from the leaf of the guava plant, Psidium guajava Linn (Myrtaceae), including the effects of two isolated flavonoid compounds, quercetin and quercetin-3-arabinoside, on withdrawal symptoms in morphine-dependent mouse models were studied. Two types of mouse narcotic-dependence models were employed.Mouse chronic narcotic-dependence model was prepared by thrice daily 1.p injection of morphine sulphate, with an initial dose of 4 mg/kg and increasing each successive dose by 4 mg/kg, up to day-9, when the dose of 108 mg/kg reached was maintained through day-11 and the morning of day-12.Mouse acutely narcotic-dependence model was prepared by a s.c. bolus injection of 50 mg/kg morphine sulphate, followed 4 h later by s.c. injection of 10 mg/kg naloxone. Manifestations of abstinence syndrome, characterised by repetitive vertical jumping,rearing,paw-shaking,head-shaking,teeth chattering,etc.,occurred about 14 h after the last dose in group A,and 45 - 60 min after naloxone injection in group B.A s.c. dose of 0.5 mg/kg of the extract, 10 h after the last dose in group A or 1 h before the naloxone injection in group B, prevented the repeated vertical jumps and greatly reduced the other symptoms of withdrawal.Parallel experiments on satiety to the extract in mice showed no development of dependence on the extract. Quercetin may be the main active compound in the extract

Inhibition of microlax- Induced experimental diarrhoea with narcotic like-extracts of psidium guajava leaf in rats

Measurement of rates of propulsion in the small intestine in control and experimental groups of male Sprague-Dawley rats (200-250 g) were carried out as a means of assessing antidiarrhoeal activity of aqueous extracts of the leaf of Psidium guajava (Linn.), using morphine as the standard drug of reference. Hyperpropulsion (diarrhoea) was induced by gavaging rats in_a control group with Microlax, using phenol red mixed into it as a marker in the intestine and the mean rate of the hyperpropulsion was determined. The normal rate of propulsioh, defined as the percentage of the length of the ileum traversed by the front of the dye in 1 h after gavaging animals·with a liquid paraffin-phenol red meal, was also determined in another control group

Susceptibility pattern of uropathogens to ciprofloxacin at the Ghana police hospital

Introduction: Reports of increasing resistance of uropathogens to antimicrobials is of global concern. Culture and drug susceptibility tests remain a vital guide to effective therapy. The aim of this study was to determine the susceptibility pattern of isolated uropathogens to ciprofloxacin at the Ghana Police Hospital. Methods: A total of 705 mid-stream urine samples were collected from patients suspected of having  urinary tract infection, and visited the Ghana Police Hospital's laboratory from December 2013 to March  2014. Samples were cultured and isolates identified by standard methods, after which isolates  susceptibility to ciprofloxacin was determined. Results: Prevalence of urinary tract infection among patients' whose samples were analyzed was 15.9%. Predominant uropathogens isolated were E .coli (46.4%), Coliform (41.1%) and Coliform spp. with Candida (6.2%). Other isolates were Pseudomonas spp. (2.7%), Salmonella spp. (1.8%), Candida spp. (0.9%) and Klebsiella spp (0.9%). The overall resistance among the top three isolated uropathogens to ciprofloxacin was 35.9%. Resistance pattern demonstrated by  respective isolates to ciprofloxacin were: E. coli (38.5%), Coliform (54.3%), and Coliform spp. with Candida (15%). The other isolates showed 100% sensitivity.Conclusion: This study revealed a relatively high ciprofloxacin resistance among isolated uropathogens,  hence, the need for prudent prescribing and use of  ciprofloxacin in urinary tract infection management.Key words: Urinary tract infection, isolates, susceptibility, antibiotics, uropathogen

Groundwater in the coastal areas of Ghana: Quality and associated health risks

Self-supply water sources, particularly groundwater sources, play key roles in the water supply ecosystem of developing countries. Recent studies indicate that groundwater sources in coastal communities in Ghana are under threat from improper waste management practices, seawater intrusion and atmospheric aerosol deposition. In this study, Water Quality Index (WQI) and Nemerow's Pollution Index (NPI) were employed to assess groundwater quality in four coastal communities of Ghana. The health risks associated with metal pollution of groundwater were investigated using incremental life cancer risk and hazard quotient. pH of groundwater in all the studied communities were acidic during the rainy season. Electrical conductivity ranged from 0.44 to 2.61 mS/cm in the rainy season and from 0.43 to 2.45 mS/cm in the dry season for the four studied locations. Results also showed brackish conditions and mineralization of groundwater in Winneba, Accra, and Keta. Mean nitrate concentrations in Winneba and Accra were higher than the WHO standards for both the rainy and the dry season. Arsenic was higher than the acceptable level in Accra and Keta during the dry season, while iron was higher than the acceptable levels in Accra in both the rainy and dry seasons. Principal Component Analyses showed that Pb, As, and Fe had the highest loading in the first component in Essiama, while PO43-and Pb had the highest loading in the second component in Accra. WQI showed that the quality of groundwater in all the studied communities ranged from marginal to poor indicating that groundwater in the coastal communities often or usually departs from desirable quality. NPI revealed that NO3- , As, and Fe contribute to groundwater deterioration. Health risk assessment showed that As posed a high cancer risk in Accra and potential cancer risk in Essiama, Winneba, and Keta during the dry season. As also posed potential cancer risk in Accra during the rainy season. Non-cancer health risk was observed for As in Accra and Keta. The findings of this study suggest urgent regulations and monitoring strategies to improve groundwater quality in the coastal communities of Ghana

Microbial Groundwater Quality Status of Hand-Dug Wells and Boreholes in the Dodowa Area of Ghana

To assess the suitability of water sources for drinking purposes, samples were taken from groundwater sources (boreholes and hand-dug wells) used for drinking water in the Dodowa area of Ghana. The samples were analyzed for the presence of fecal indicator bacteria (Escherichia coli) and viruses (Adenovirus and Rotavirus), using membrane filtration with plating and glass wool filtration with quantitative polymerase chain reaction (PCR), respectively. In addition, sanitary inspection of surroundings of the sources was conducted to identify their vulnerability to pollution. The presence of viruses was also assessed in water samples from the Dodowa River. More than 70% of the hand-dug wells were sited within 10 m of nearby sources of contamination. All sources contained E. coli bacteria, and their numbers in samples of water between dug wells and boreholes showed no significant difference (p = 0.48). Quantitative PCR results for Adenovirus indicated 27% and 55% were positive for the boreholes and hand-dug wells, respectively. Samples from all boreholes tested negative for the presence of Rotavirus while 27% of the dug wells were positive for Rotavirus. PCR tests of 20% of groundwater samples were inhibited. Based on these results we concluded that there is systemic microbial and fecal contamination of groundwater in the area. On-site sanitation facilities, e.g., pit latrines and unlined wastewater drains, are likely the most common sources of fecal contamination of groundwater in the area. Water abstracted from groundwater sources needs to be treated before use for consumption purposes. In addition, efforts should be made to delineate protected areas around groundwater abstraction points to minimize contamination from point sources of pollution

Susceptibility pattern of uropathogens to ciprofloxacin at the Ghana police hospital

Abstract Introduction: Reports of increasing resistance of uropathogens to antimicrobials is of global concern. Culture and drug susceptibility tests remai

Contributing factors to unsafe abortion practices among women of reproductive age at selected district hospitals in the Ashanti region of Ghana

Background Despite the existence of an abortion law and a safe abortion policy in Ghana, the Ghana Statistical Service found that 15% of all women in the reproductive age group (15–49 years) have practiced unsafe abortions. The objective of this study was to explore factors that contribute to the high incidence of unsafe abortion practices in the Ashanti Region of Ghana. Methods A qualitative descriptive study design was used to assess factors that contribute to unsafe abortion practices. Purposive sampling technique was employed in selecting participants. Data were collected through key informant interviews and focus group discussions. One hundred and eleven participants were involved in the study. Data analysis was carried out through qualitative content analysis. Results Seven thematic categories were elicited from data collected. The categories are: a) Lack of knowledge of safe abortion services; b) Socio-economic conditions as a perceived influence for unsafe abortion practices; c) Safe abortion as a perceived religious and cultural taboo in Ghana; d) Stigma of unplanned pregnancy; e) A desire to bear children only after marriage; f) Avoiding parental/guardian disappointment and resentment; g) A desire to pursue education. Conclusions Evidence available in this study suggests that several factors are responsible for unsafe abortion practices in Ghana. Lack of knowledge on safe abortion services, poor socio-economic conditions, cultural and religious beliefs, a stigma of unplanned pregnancy, a desire to bear children only after marriage, attempts to avoid parental/guardian disappointment and resentment, and a desire to pursue education were cited by participants as situations that contributed to unsafe abortion practices. Measures such as Aunty Jane, Ms. Rose and Women Help Women programmes can be publicised to reduce maternal morbidity and mortality that occur as a result of unsafe abortions in Ghana. Improvement in family planning education in educational institutions needs to be considered in order to reduce the rate of unwanted pregnancies among young women in school