10,385 research outputs found
Extracellular Sodium Interacts with the HERG Channel at an Outer Pore Site
Most voltage-gated K+ currents are relatively insensitive to extracellular Na+ (Na+o), but Na+o potently inhibits outward human ether-a-go-go–related gene (HERG)–encoded K+ channel current (Numaguchi, H., J.P. Johnson, Jr., C.I. Petersen, and J.R. Balser. 2000. Nat. Neurosci. 3:429–30). We studied wild-type (WT) and mutant HERG currents and used two strategic probes, intracellular Na+ (Na+i) and extracellular Ba2+ (Ba2+o), to define a site where Na+o interacts with HERG. Currents were recorded from transfected Chinese hamster ovary (CHO-K1) cells using the whole-cell voltage clamp technique. Inhibition of WT HERG by Na+o was not strongly dependent on the voltage during activating pulses. Three point mutants in the P-loop region (S624A, S624T, S631A) with intact K+ selectivity and impaired inactivation each had reduced sensitivity to inhibition by Na+o. Quantitatively similar effects of Na+i to inhibit HERG current were seen in the WT and S624A channels. As S624A has impaired Na+o sensitivity, this result suggested that Na+o and Na+i act at different sites. Extracellular Ba2+ (Ba2+o) blocks K+ channel pores, and thereby serves as a useful probe of K+ channel structure. HERG channel inactivation promotes relief of Ba2+ block (Weerapura, M., S. Nattel, M. Courtemanche, D. Doern, N. Ethier, and T. Hebert. 2000. J. Physiol. 526:265–278). We used this feature of HERG inactivation to distinguish between simple allosteric and pore-occluding models of Na+o action. A remote allosteric model predicts that Na+o will speed relief of Ba2+o block by promoting inactivation. Instead, Na+o slowed Ba2+ egress and Ba2+ relieved Na+o inhibition, consistent with Na+o binding to an outer pore site. The apparent affinities of the outer pore for Na+o and K+o as measured by slowing of Ba2+ egress were compatible with competition between the two ions for the channel pore in their physiological concentration ranges. We also examined the role of the HERG closed state in Na+o inhibition. Na+o inhibition was inversely related to pulsing frequency in the WT channel, but not in the pore mutant S624A
Neutrino-Mixing-Generated Lepton Asymmetry and the Primordial He Abundance
It has been proposed that an asymmetry in the electron neutrino sector may be
generated by resonant active-sterile neutrino transformations during Big Bang
Nucleosynthesis (BBN). We calculate the change in the primordial He yield
resulting from this asymmetry, taking into account both the time evolution
of the and distribution function and the spectral
distortions in these. We calculate this change in two schemes: (1) a lepton
asymmetry directly generated by mixing with a lighter right-handed
sterile neutrino ; and (2) a lepton asymmetry generated by a
or transformation
which is subsequently partially converted to an asymmetry in the
sector by a matter-enhanced active-active neutrino
transformation. In the first scheme, we find that the percentage change in
is between -1% and 9% (with the sign depending on the sign of the asymmetry),
bounded by the Majorana mass limit m_{\nu_e}\la 1 eV. In the second scheme,
the maximal percentage reduction in is 2%, if the lepton number asymmetry
in neutrinos is positive; Otherwise, the percentage increase in is \la 5%
for m^2_{\nu_\mu,\nu_\tau}-m^2_{\nu_s}\la 10^4 eV. We conclude that the
change in the primordial He yield induced by a neutrino-mixing-generated
lepton number asymmetry can be substantial in the upward direction, but limited
in the downward direction.Comment: 15 pages, 7 figures, submitted to PR
Identification of α°-thalassaemia (–SEA) using an enzyme-linked immunosorbent assay (ELISA) for embryonic zeta-globin chain detection – a preliminary study
Objectives: This study aimed to evaluate the UBI MAGIWELTM ζ-GLOBIN ELISA Kit for the presumptive diagnosis of αo-thalassaemia. The ELISA results obtained were confirmed by molecular characterisation of αo-thalassaemia using a Duplex-PCR. Methods: Routine peripheral blood counts and red cell indices were determined in 94 blood samples sent for Hb analysis. Hb subtypes were quantified by high performance liquid chromatography (HPLC) and Hb electrophoresis conducted on agarose gel at pH 8.5. Zeta-globin chain levels were determined using the UBI MAGIWELTM ζ-GLOBIN ELISA Kit. Molecular analysis was performed using a duplex-PCR which simultaneously amplifies a normal 136 bp sequence between the ψα−α2-globin genes and a 730 bp Southeast Asian deletion-specific sequence (–SEA) between the ψα2−θ1-globin genes. Results: Using the ELISA assay kit, 20 blood samples were presumptively identified as α-thalassaemia carriers from elevated ζ-globin chains (OD>0.3) while the remaining 74 blood samples showed OD<0.3. Molecular characterisation confirmed amplification of the 136 bp normal fragment in all the blood samples. Seventeen of the 20 DNA samples from the α-thalassaemia carriers amplified the SEA-deletion specific fragment. The remaining three DNA samples did not amplify the SEA-deletion specific fragment but amplified the normal α-globin gene sequence, indicating the presence of amplifiable DNA in these samples. Further molecular characterisation of the α-3.7 and -α4.2 deletions and Hb Constant Spring (CS) mutation showed the absence of these defects in these 3 DNA samples. Conclusion: This preliminary investigation showed the sensitivity and specificity of the UBI MAGIWEL ζ-globin ELISA kit as 100 % and 96.1 % respectively in the detection of α-thalassaemia-1 carriers (–SEA)
Dynamical Mass Generation in a Finite-Temperature Abelian Gauge Theory
We write down the gap equation for the fermion self-energy in a
finite-temperature abelian gauge theory in three dimensions. The instantaneous
approximation is relaxed, momentum-dependent fermion and photon self-energies
are considered, and the corresponding Schwinger-Dyson equation is solved
numerically. The relation between the zero-momentum and zero-temperature
fermion self-energy and the critical temperature T_c, above which there is no
dynamical mass generation, is then studied. We also investigate the effect
which the number of fermion flavours N_f has on the results, and we give the
phase diagram of the theory with respect to T and N_f.Comment: 20 LaTeX pages, 4 postscript figures in a single file, version to
appear in Physical Review
State Orthogonalization by Building a Hilbert Space: A New Approach to Electronic Quantum Transport in Molecular Wires
Quantum descriptions of many complex systems are formulated most naturally in
bases of states that are not mutually orthogonal. We introduce a general and
powerful yet simple approach that facilitates solving such models exactly by
embedding the non-orthogonal states in a new Hilbert space in which they are by
definition mutually orthogonal. This novel approach is applied to electronic
transport in molecular quantum wires and is used to predict conductance
antiresonances of a new type that arise solely out of the non-orthogonality of
the local orbitals on different sites of the wire.Comment: 4 pages 1 figur
Multiple tumor suppressors regulate a HIF-dependent negative feedback loop via ISGF3 in human clear cell renal cancer.
Whereas VHL inactivation is a primary event in clear cell renal cell carcinoma (ccRCC), the precise mechanism(s) of how this interacts with the secondary mutations in tumor suppressor genes, including PBRM1, KDM5C/JARID1C, SETD2, and/or BAP1, remains unclear. Gene expression analyses reveal that VHL, PBRM1, or KDM5C share a common regulation of interferon response expression signature. Loss of HIF2α, PBRM1, or KDM5C in VHL-/-cells reduces the expression of interferon stimulated gene factor 3 (ISGF3), a transcription factor that regulates the interferon signature. Moreover, loss of SETD2 or BAP1 also reduces the ISGF3 level. Finally, ISGF3 is strongly tumor-suppressive in a xenograft model as its loss significantly enhances tumor growth. Conversely, reactivation of ISGF3 retards tumor growth by PBRM1-deficient ccRCC cells. Thus after VHL inactivation, HIF induces ISGF3, which is reversed by the loss of secondary tumor suppressors, suggesting that this is a key negative feedback loop in ccRCC. © 2018, Liao et al
A Survey of Local Group Galaxies Currently Forming Stars. I. UBVRI Photometry of Stars in M31 and M33
We present UBVRI photometry obtained from Mosaic images of M31 and M33 using
the KPNO 4-m telescope. The survey covers 2.2 sq degrees of M31, and 0.8 sq
degrees of M33, chosen so as to include all of the regions currently active in
forming massive stars. The catalog contains 371,781 and 146,622 stars in M31
and M33, respectively, where every star has a counterpart (at least) in B, V,
and R. We compare our photometry to previous studies. We provide cross
references to the stars confirmed as members by spectroscopy, and compare the
location of these to the complete set in color-magnitude diagrams. While
follow-up spectroscopy is needed for many projects, we demonstrate the success
of our photometry in being able to distinguish M31/M33 members from foreground
Galactic stars. We also present the results of newly obtained spectroscopy,
which identifies 34 newly confirmed members, including B-A supergiants, the
earliest O star known in M31, and two new Luminous Blue Variable candidates
whose spectra are similar to that of P Cygni.Comment: Accepted by the Astronomical Journal. A version with higher
resolution figures can be found at:
http://www.lowell.edu/users/massey/M3133.pdf.g
Suitability versus fidelity for rating single-photon guns
The creation of specified quantum states is important for most, if not all,
applications in quantum computation and communication. The quality of the state
preparation is therefore an essential ingredient in any assessment of a
quantum-state gun. We show that the fidelity, under the standard definitions is
not sufficient to assess quantum sources, and we propose a new measure of
suitability that necessarily depends on the application for the source. We
consider the performance of single-photon guns in the context of quantum key
distribution (QKD) and linear optical quantum computation. Single-photon
sources for QKD need radically different properties than sources for quantum
computing. Furthermore, the suitability for single-photon guns is discussed
explicitly in terms of experimentally accessible criteria.Comment: 4 pages, 2 figures Revised per referee suggestion
Planetary Nebulae as standard candles XI. Application to Spiral Galaxies
We report the results of an [O III] lambda 5007 survey for planetary nebulae
(PN) in three spiral galaxies: M101 (NGC 5457), M51 (NGC 5194/5195) and M96
(NGC 3368). By comparing on-band/off-band [O III] lambda 5007 images with
images taken in H-alpha and broadband R, we identify 65, 64 and 74 PN
candidates in each galaxy, respectively. From these data, an adopted M31
distance of 770 kpc, and the empirical planetary nebula luminosity function
(PNLF), we derive distances to M101, M51, and M96 of 7.7 +/- 0.5, 8.4 +/- 0.6,
and 9.6 +/- 0.6 Mpc. These observations demonstrate that the PNLF technique can
be successfully applied to late-type galaxies, and provide an important overlap
between the Population I and Population II distance scales. We also discuss
some special problems associated with using the PNLF in spiral galaxies,
including the effects of dust and the possible presence of [O III] bright
supernova remnants.Comment: 38 pages, TeX, with tables included but not figures. Uses epsf.tex
and kpnobasic.tex. To be published in the Astophysical Journal. Full paper is
available at http://www.astro.psu.edu/users/johnf/Text/research.htm
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