Statistical analysis of quantitative seroepidemiological data

The material for this thesis is based on the European Sero-Epidemiology Network 2 (ESEN2), a study funded by the European Commission. As part of the ESEN2 project, age-specific population seroprevalence of 8 antigens tested in 22 national laboratories was estimated and compared between countries. To achieve harmonised serological results, a reference panel was tested by each participant laboratory. Each laboratory's panel results were regressed against the reference centre, thus obtaining standardisation equations. These equations were used to convert the quantitative measurements of the serosurveys into common units that were subsequently classified into negative (susceptible) or positive (protected) according to a serological cut-off. The aim of this thesis was to further develop and validate the methodology for standardising serological outcomes, and to propose alternative methods for achieving comparable population seroprevalence. As part of this thesis, a statistical algorithm was established to standardise serological results. Censored regression methods were considered to account for measurements outside the assay detection range. The impact of standardisation on seroprevalence was examined. Mixture modelling of the serological results was proposed as an alternative method to standardisation for estimating seroprevalence. Although mixture modelling may provide better seroprevalence estimates in certain situations, it is heavily dependent on model assumptions, mainly of well-separated underlying distributions. In terms of seroprevalence estimation using standardisation, the validity of the assay cut-off point was examined. A method for re-estimating cut-offs was proposed based on mixture modelling that improved seroprevalence estimates under certain distributional assumptions. The impact of variability occurring due to serum testing in batches (plate-to-plate variability) on seroprevalence was assessed. The method currently used by the laboratories was examined, and a new method was proposed to adjust for this based on mixture models. In conclusion, the standardisation method used for the ESEN2 project was validated and some improvements were proposed

Analyzing Black Cloud Dynamics over Cairo, Nile Delta Region and Alexandria using Aerosols and Water Vapor Data

Cairo is the largest city of Africa and one of the world’s megacities, with a population of more than 20 million people and containing more than one third of the national industry. It is a rapidly expanding city which leads to many associated environmental problems. As a result, it is also one of the most air polluted megacities in the world (Molina and Molina, 2004). It suffers from high ambient concentrations of atmospheric pollutants including particulates (PM), carbon monoxide, nitrogen oxides, ozone and sulfur dioxide (Abu-Allaban et al., 2007, Abu-Allaban et al., 2002, El-Metwally et al., 2008). The pollution phenomenon locally known as “Black cloud” over Cairo has been attributed to many reasons among which are biomass burning, local emission and long range transport during the fall season.Several studies have been conducted to address and discuss the forth mentioned reasons for the increased pollution levels over Cairo and the greater Delta region using ground-based and satellite air quality data as compared to other megacities.https://digitalcommons.chapman.edu/sees_books/1000/thumbnail.jp

Effects of Greek orthodox christian church fasting on serum lipids and obesity

BACKGROUND: No study to date has focused on the impact of Greek Orthodox Christian fasting on serum lipoproteins and obesity yet. METHODS: 120 Greek adults were followed longitudinally for one year. Sixty fasted regularly in all fasting periods (fasters) and 60 did not fast at all (controls). The three major fasting periods under study were: Christmas (40 days), Lent (48 days) and Assumption (August, 15 days). A total of 6 measurements were made during one year including pre- and end-fasting blood collection, serum lipoprotein analyses and anthropometric measurements. RESULTS: Statistically significant end-fasting total and LDL cholesterol differences were found in fasters. Fasters compared to controls presented 12.5% lower end-total cholesterol (p < 0.001), 15.9% lower end-LDL cholesterol (p < 0.001) and 1.5% lower end-BMI (p < 0.001). The end- LDL/HDL ratio was lower in fasters (6.5%, p < 0.05) while the change in end- HDL cholesterol in fasters (4.6% decline) was not significant. Similar results were found when the pre- and end-fasting values of fasters were compared. No change was found in control subjects. CONCLUSIONS: Adherence to Greek Orthodox fasting periods contributes to a reduction in the blood lipid profile including a non-significant reduction in HDL cholesterol and possible impact on obesity

Observations on the Stress Related Variations of Soil Radon Concentration in the Gulf of Corinth, Greece

Our observations indicate a characteristic pattern in the long-term variation of soil radon concentrations, which seems to be consistent with the expected variation of regional stress in relation to seismicity. However, it seems that the major changes in radon level begin before the rock rapture, i.e. before the earthquake occurs. These conclusions have emerged after long-term observations with continuous and thorough real-time gamma-radiation monitoring in the seismically active area of the Gulf of Corinth, Greece. The recordings acquired close to a hot spring were of very high quality, implying that the deep hydraulic flow can possibly play a key role in the pre-earthquake variation of radon level. We were able to observe outstanding examples of radon level variations before significant seismic events in the Gulf of Corinth that cannot be attributed to other external factors such as atmospheric phenomena

Hepatitis E Outbreak on Cruise Ship

The outbreak was probably foodborne

Dietary and other lifestyle correlates of serum folate concentrations in a healthy adult population in Crete, Greece: a cross-sectional study

BACKGROUND: Folate has emerged as a key nutrient for optimising health. Impaired folate status has been identified as a risk factor for cardiovascular disease, various types of cancers, and neurocognitive disorders. The study aimed at examining the distribution and determinants of serum folate concentrations in a healthy adult population in Crete, Greece. METHODS: A cross-sectional sample of 486 healthy adults (250 men, 236 women) aged 39 ± 14 years, personnel of the Medical School and the University Hospital of Crete in Greece, was examined. Serum folate and vitamin B(12 )concentrations were measured by microbiological assay, and total homocysteine was determined fluorometrically and by high-pressure liquid chromatography. Lifestyle questionnaires were completed, and nutrient intakes and food consumption were assessed by 24-h dietary recalls. Multivariate analyses were performed using SPSS v10.1. RESULTS: The geometric mean (95% confidence interval) concentrations of serum folate were 15.6 μmol/l (14.6–16.8) in men and 19.2 μmol/l (17.9–20.7) in women (p < 0.001). Inadequate folate levels (≤7 nmol/l) were present in 6.8% of men and 2.1% of women (p < 0.001). Approximately 76% of men and 87% of women did not meet the reference dietary intake for folate (400 μg/day). Serum folate was inversely related to total homocysteine levels (p < 0.001). Increased tobacco and coffee consumption were associated with lower folate concentrations (p < 0.05 for both) but these associations disappeared after controlling for nutrient intakes. In multivariate analysis, intakes of MUFA, fibre, calcium, magnesium, folate, and vitamins A, E, C, B(1), and B(6 )were positively associated with serum folate. Consumption of potatoes, legumes, fruits, and vegetables were favourably related to the serum folate status. CONCLUSION: Serum folate concentrations were associated with various demographic, lifestyle and dietary factors in healthy Cretan adults. Large-scale epidemiological studies should be conducted within the general Greek adult population to assess the prevalence of impaired folate status and further examine associations with dietary patterns and chronic disease risk. Considering the importance of folate in health maintenance, it is important to increase the public's awareness of modifiable lifestyle patterns and diet and tobacco use in particular, which may be associated with improved folate status

The Evolution of the Anopheles 16 Genomes Project

We report the imminent completion of a set of reference genome assemblies for 16 species of Anopheles mosquitoes. In addition to providing a generally useful resource for comparative genomic analyses, these genome sequences will greatly facilitate exploration of the capacity exhibited by some Anopheline mosquito species to serve as vectors for malaria parasites. A community analysis project will commence soon to perform a thorough comparative genomic investigation of these newly sequenced genomes. Completion of this project via the use of short next-generation sequence reads required innovation in both the bioinformatic and laboratory realms, and the resulting knowledge gained could prove useful for genome sequencing projects targeting other unconventional genomes

Cosmic ray neon, Wolf-Rayet stars, and the superbubble origin of galactic cosmic rays

The abundances of neon isotopes in the galactic cosmic rays (GCRs) are reported using data from the Cosmic Ray Isotope Spectrometer (CRIS) aboard the Advanced Composition Explorer (ACE). We compare our ACE-CRIS data for neon and refractory isotope ratios, and data from other experiments, with recent results from two-component Wolf-Rayet (WR) models. The three largest deviations of GCR isotope ratios from solar-system ratios predicted by these models are indeed present in the GCRs. Since WR stars are evolutionary products of OB stars, and most OB stars exist in OB associations that form superbubbles, the good agreement of these data with WR models suggests that superbubbles are the likely source of at least a substantial fraction of GCRs.Comment: 22 pages, 6 figures Accepted for publication by Ap

Overweight and obesity in relation to cardiovascular disease risk factors among medical students in Crete, Greece

BACKGROUND: Recent data indicate increasing rates of adult obesity and mortality from cardiovascular disease (CVD) in Greece. No data, however, are available on prevalence of overweight and obesity in relation to CVD risk factors among young adults in Greece. METHODS: A total of 989 third-year medical students (527 men, 462 women), aged 22 ± 2 years, were recruited from the University of Crete during the period 1989–2001. Anthropometric measures and blood chemistries were obtained. The relationships between obesity indices (body mass index [BMI], waist circumference [WC], waist-to-hip ratio [WHpR], waist-to-height ratio [WHtR]) and CVD risk factor variables (blood pressure, glucose, serum lipoproteins) were investigated. RESULTS: Approximately 40% of men and 23% of women had BMI ≥ 25.0 kg/m(2). Central obesity was found in 33.4% (average percentage corresponding to WC ≥ 90 cm, WHpR ≥ 0.9 and WHtR ≥ 50.0) of male and 21.7% (using WC ≥ 80 cm, WHpR ≥ 0.8, WHtR ≥ 50.0) of female students. Subjects above the obesity indices cut-offs had significantly higher values of CVD risk factor variables. BMI was the strongest predictor of hypertension. WHtR in men and WC in women were the most important indicators of dyslipidaemia. CONCLUSION: A substantial proportion of Greek medical students were overweight or obese, obesity status being related to the presence of hypertension and dyslipidaemia. Simple anthropometric indices can be used to identify these CVD risk factors. Our results underscore the need to implement health promotion programmes and perform large-scale epidemiological studies within the general Greek young adult population

Use of Antiviral Drugs to Reduce Household Transmission of Pandemic (H1N1) 2009, United Kingdom1

TOC Summary: Early treatment of primary case-patients and prophylaxis of household contacts provides effective protection