Management and Modeling of Winter-time Basil Cultivars Grown with a Cap MAT System

Basil (Ocimum basilicum) is a high value crop, currently grown in the field and greenhouses in Nebraska. Winter-time, greenhouse studies were conducted during 2015 and 2016, focusing on cultivars of basil grown on a Cap MAT II® system with various levels of fertilizer application. The goal was to select high value cultivars that could be grown in Nebraska greenhouses. The studies used water content, electrical conductivity, photosynthetically active radiation (PAR), and relative humidity, air and soil media temperature sensors. Greenhouse systems can be very complex, even though controlled by mechanical heating and cooling. Uncertain or ambiguous environmental and plant growth factors can occur, where growers need to plan, adapt, and react appropriately. Plant harvest weights and electronic sensor data was recorded over time and used for training and internally validating fuzzy logic inference and classification models. Studies showed that GENFIS2 ‘subtractive clustering’ of data, prior to ANFIS training, resulted in good correlations for predicted growth (R2 \u3e 0.85), with small numbers of effective rules and membership functions. Cross-validation and internal validation studies also showed good correlations (R2 \u3e 0.85). Decisions on basil cultivar selection and forecasting as to how quickly a basil crop will reach marketable size will help growers to know when to harvest, for optimal yield and predictable quantity of essential oils. If one can predict reliably how much essential oil will be produced, then the methods and resultant products can be proposed for USP or FDA approval. Currently, most plant medicinal and herbal oils and other supplements vary too widely in composition for approval. The use of fuzzy set theory could be a useful mathematical tool for plant and horticultural production studies

Management and Modeling of Winter-time Basil Cultivars Grown with a Cap MAT System

Basil (Ocimum basilicum) is a high value crop, currently grown in the field and greenhouses in Nebraska. Winter-time, greenhouse studies were conducted during 2015 and 2016, focusing on cultivars of basil grown on a Cap MAT II® system with various levels of fertilizer application. The goal was to select high value cultivars that could be grown in Nebraska greenhouses. The studies used water content, electrical conductivity, photosynthetically active radiation (PAR), and relative humidity, air and soil media temperature sensors. Greenhouse systems can be very complex, even though controlled by mechanical heating and cooling. Uncertain or ambiguous environmental and plant growth factors can occur, where growers need to plan, adapt, and react appropriately. Plant harvest weights and electronic sensor data was recorded over time and used for training and internally validating fuzzy logic inference and classification models. Studies showed that GENFIS2 ‘subtractive clustering’ of data, prior to ANFIS training, resulted in good correlations for predicted growth (R2 \u3e 0.85), with small numbers of effective rules and membership functions. Cross-validation and internal validation studies also showed good correlations (R2 \u3e 0.85). Decisions on basil cultivar selection and forecasting as to how quickly a basil crop will reach marketable size will help growers to know when to harvest, for optimal yield and predictable quantity of essential oils. If one can predict reliably how much essential oil will be produced, then the methods and resultant products can be proposed for USP or FDA approval. Currently, most plant medicinal and herbal oils and other supplements vary too widely in composition for approval. The use of fuzzy set theory could be a useful mathematical tool for plant and horticultural production studies

In vitro scanning saturation mutagenesis of proteins

The present invention combines PCR.TM. mutagenesis with in vitro transcription/translation and ELISA for the rapid generation and characterization of protein mutants. The PCR.TM. products are used directly as the template for the in vitro transcription/translation reactions and because no cloning steps are required, the in vitro saturation mutagenesis of one residue can be completed in duplicate within a week by a single investigator. This high throughput enables the saturation mutagenesis of numerous residues of interest, a process that can be described as in vitro scanning saturation mutagenesis. Compositions and methods of use of such a process are described herein.Board of Regents, University of Texas Syste

Increasing Hospital Admissions for Pneumonia, England

This rise in recorded incidence from 2001 to 2005 was particularly marked among the elderly

B835: Landfills and Municpal Solid Waste in Maine

Municipal leaders need current information about alternative disposal methods to make rational decisions on handling their town\u27s waste. To provide an overview of landfilling and other waste-handling methods used in the upper New England states, a group of university researchers from New Hampshire, Maine, and Vermont initiated a study of landfills and solid waste management practices. The study involved a comprehensive mail survey of municipalities in Maine, New Hampshire, and Vermont. This report focuses upon and discusses the results of the landfill and solid waste management survey for Maine.https://digitalcommons.library.umaine.edu/aes_bulletin/1019/thumbnail.jp

Incorporation of prefabricated screw, pneumatic, and solenoid valves into microfluidic devices

This paper describes a method for prefabricating screw, pneumatic, and solenoid valves and embedding them in microfluidic devices. This method of prefabrication and embedding is simple, requires no advanced fabrication, and is compatible with soft lithography. Because prefabrication allows many identical valves to be made at one time, the performance across different valves made in the same manner is reproducible. In addition, the performance of a single valve is reproducible over many cycles of opening and closing: an embedded solenoid valve opened and closed a microfluidic channel more than 100,000 times with no apparent deterioration in its function. It was possible to combine all three types of prefabricated valves in a single microfluidic device to control chemical gradients in a microfluidic channel temporally and spatially