3 research outputs found
Simultaneous Detection of Androgen and Estrogen Abuse in Breeding Animals by Gas Chromatography–Mass Spectrometry/Combustion/Isotope Ratio Mass Spectrometry (GC-MS/C/IRMS) Evaluated against Alternative Methods
The administration of synthetic homologues
of naturally occurring
steroids can be demonstrated by measuring <sup>13</sup>C/<sup>12</sup>C isotopic ratios of their urinary metabolites. Gas chromatography–mass
spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS)
was used in this study to appraise in a global approach isotopic deviations
of two 17β-testosterone metabolites (17α-testosterone
and etiocholanolone) and one 17β-estradiol metabolite (17α-estradiol)
together with those of 5-androstene-3β,17α-diol as endogenous
reference compound (ERC). Intermediate precisions of 0.35‰,
1.05‰, 0.35‰, and 0.21‰, respectively, were observed
(<i>n</i> = 8). To assess the performance of the analytical
method, a bull and a heifer were treated with 17β-testosterone
propionate and 17β-estradiol-3-benzoate. The sensitivity of
the method permitted the demonstration of 17β-estradiol treatment
up to 24 days. For 17β-testosterone treatment, the detection
windows were 3 days and 24 days for the bull and the heifer, respectively.
The capability of GC-MS/C/IRMS to demonstrate natural steroid abuse
for urinary steroids was eventually compared to those of mass spectrometry
(LC-MS/MS) when measuring intact steroid esters in blood and hair
De Cuyper et al_RawDataPredatorPreyRatios
Data spreadsheet including all raw literature data used for the scaling relationships predator size vs prey size, pack size, real observed kill frequency and gut capacity (see corresponding tabs). Legends are included to explain column headings (see corresponding tabs). Additionally, prey weights obtained from internet sources are provided in the spreadsheet
Additional file 1: of Does canine inflammatory bowel disease influence gut microbial profile and host metabolism?
Canine chronic entreropathy clinical activity index in IBD dogs. Description of data: the total CCECAI score and the individual score of each criterion. (XLSX 9 kb