Yellow necked mouse (Apodemus flavicollis), wood mouse (Apodemus sylvaticus), Eurasian harvest mouse (Micromys minutes) genetic diversity researches

Using inter-simple sequence repeat and polymerase chain reaction technique 125 Apodemus flavicollis speciments were studied and 11 Apodemus sylvaticus from eighteen different populations from two different countries of Europe (Norway, Lithuania). In the work nine molecular markers were tested: (E8S (exone 8) Ep8-1 (exone 8), c-myc-1 (exone 3), Ap-1, AP-2, AP-3, AP-4, AP-5, AP- 6), to choose the suitable markers for Apodemus genus species identification. Also from these nine molecular markers three were choosen (AP-1, AP-2, AP-5) to study genetic diversity. Data analysis was perform by PopGene3.2, TREECON GenAlEx 6b4 computer programs. Genetic variability, DNA polymorphism, polymorphic locus, allele and unique allele numbers, genetic diversity was estimated within populations and among populations. Phylogenetic trees of populations using data results were paintedBiologijos katedraGamtos mokslų fakultetasKauno Tado Ivanausko zoologijos muziejusVytauto Didžiojo universiteta

Genetic diversity Apodemus sylvaticus and Apodemus flavicollis in Lithuania

Mice of genus Apodemus are wide spred in Europe, western Asia and North Africa. Twenty one species are now recognized, but morfological similarity makes difficulties to recognize these species. Therer is a requirement of molecular markers to clear species identification. The aim of our investigation was to evalute genetic diversity of A. sylvaticus and A. flavicollis in Lithuania using ISSR markers. For DNA research was captured 45 mices from Kaunas district, 13 from Zemaitija National park, 130 rodents from Neringa, 13 from Kaisiadoriai district, and 49 from Juodkrante. Also for research using other mouse from previos Vytautas Magnus University Natural science falculty expeditions from Norway, where were captured 15 Apodemus genus mouse. Using chloroform method „Genomic DNA Purification Kit“(Fermentas, K0512). DNA was extracted from ears and muscules for genome studies. Chosen primers: AP-1 (5‘-(CAG)5GC-3‘), AP-2 (5‘-(CAG)4AC-3’), AP-5 (5‘-(G)6GC-3’), E8S(5‘-TAAATGGGACAGGTAGGACC-3’) and Ep8 1 (5‘CCTTACTGCCTCTTGCTTC-3’). For PCR data analysis used horizontal 1,5% agarose gels. The size of the fragments ranged from 300 to 1700 bp. The primers AP-2, AP-5 and E8S were the most informative to study genetic diversyti of genus ApodemusBiologijos katedraTado Ivanausko zoologijos muziejusVytauto Didžiojo universiteta

Flea diversity in small mammals from peninsula Kuršiu Nerija of Lithuania

In our study from 2011 through 2012 small mammals were analysed for their flea fauna. Small mammals were captured in the peninsula Kuršiu Nerija of Lithuania. One hundred and eighty four fleas were collected from 128 small mammals, including 64 Apodemus flavicollis, 3 Apodemus sylvaticus, 11 Myodes glareolus, 9 Microtus arvalis, 4 Microtus oeconomus, 30 Micromys minutes and 7 Sorex minutus. Five species of fleas were described: Hystrichopsylla t. talpae, Ctenophthalmus a. agyrtes, Megabothris turbidus, Megabothris walkeri and Palaeopsylla soricis, and parasitological terms by fleas were studiedBiologijos katedraGamtos mokslų fakultetasKauno Tado Ivanausko zoologijos muziejusVytauto Didžiojo universiteta

Hazel dormouse (Muscardinus avellanarius) genetic variability research In Lithuania

A preliminary genetic analysis of common dormouse was conducted on 17 samples (13 were taken from 9 different Lithuania locations and 4 samples from Latvia) collected in 2013-2014. DNA was extracted from hair using the DNeasy Tissue kit (Qiagen Inc., Valencia, CA, USA) following the manufacturer‘s instructions. In order to assess the genetic diversity of the common dormouse 5 microsatellite loci were used (Mav002, Mav005, Mav011, Mav023 and Mav028). PAAG electrophoresis results showed 41 polymorphic loci with fragment size - from 120 to 300 base pairs. Data genetic variability were evaluated using GenAlEx and MEGA 6.06 software. Neither's genetic distance between dormouse populations studied ranged from 0.291 to 1.219Biologijos katedraGamtos mokslų fakultetasKauno Tado Ivanausko zoologijos muziejusVytauto Didžiojo universiteta

Fat (Glis glis), forest (Dryomys nitedula) and common (Muscardinus Avellanarius) dormouse abundance in Kaunas region

Dormice are unique rodents due to hibernation, estivation and daily torpor. At this moment three dormouse species of four are recorded into Lithuania red book (2007): garden dormouse – 0 (Ex) category (Extinct/ believed to be extinct), fat dormouse – 2 (V) category (vulnerable species), and forest dormouse belongs to 3 (R) category (rare species). It is very important to know more about number of these endangered animals. In present study monitoring of dormouse were used more than 200 nestboxes in 6 different localities in Kaunas district (Būdų forest, Šešuva preserve, Vadų forest, Pravieniškių forest, Strošiūnų forest) during 2010 – 2013 years. After 2013 monitoring there were found 61 dormouse (30 fat dormouse, 23 forest dormouse and 8 common dormouse), all of them were weighed, sexed and aged. During 2010 – 2013 years were caught more than 240 dormouse (91 fat dormouse, 90 forest dormouse and 59 common dormouse. During monitoring it was concluded that slot-loading number better show dormouse population size than the set number of nestboxes and not on the same time collected data can skew the results of dormouse population sizeBiologijos katedraKauno Tado Ivanausko zoologijos muziejusVytauto Didžiojo universiteta

Molecular phylogeography and genetic structure of alien species raccoon dog (Nyctereutes procyonoides) in Europe

Biologijos katedraKauno Tado Ivanausko zoologijos muziejusVytauto Didžiojo universiteta

Data on morphometry of raccoon dogs (Nyctereutes procyonoides) from Lithuania

Biologijos katedraVytauto Didžiojo universiteta

Fleas (Siphonaptera) infections with Bartonella in Lithuania

Biologijos katedraKauno Tado Ivanausko zoologijos muziejusVytauto Didžiojo universiteta

Usūrinių šunų (Nyctereutes procyonoides) kaukolių morfometrinių duomenų skirtumai

Straipsnio antraštėje klaida. Turėtų būti: Variability of skull morphometric characters in Nyctreutes procyonoidesBiologijos katedraKauno Tado Ivanausko zoologijos muziejusVytauto Didžiojo universiteta