Yellow necked mouse (Apodemus flavicollis), wood mouse (Apodemus sylvaticus), Eurasian harvest mouse (Micromys minutes) genetic diversity researches

Abstract

Using inter-simple sequence repeat and polymerase chain reaction technique 125 Apodemus flavicollis speciments were studied and 11 Apodemus sylvaticus from eighteen different populations from two different countries of Europe (Norway, Lithuania). In the work nine molecular markers were tested: (E8S (exone 8) Ep8-1 (exone 8), c-myc-1 (exone 3), Ap-1, AP-2, AP-3, AP-4, AP-5, AP- 6), to choose the suitable markers for Apodemus genus species identification. Also from these nine molecular markers three were choosen (AP-1, AP-2, AP-5) to study genetic diversity. Data analysis was perform by PopGene3.2, TREECON GenAlEx 6b4 computer programs. Genetic variability, DNA polymorphism, polymorphic locus, allele and unique allele numbers, genetic diversity was estimated within populations and among populations. Phylogenetic trees of populations using data results were paintedBiologijos katedraGamtos mokslų fakultetasKauno Tado Ivanausko zoologijos muziejusVytauto Didžiojo universiteta