The human ARF tumor suppressor senses blastema activity and suppresses epimorphic tissue regeneration.

The control of proliferation and differentiation by tumor suppressor genes suggests that evolution of divergent tumor suppressor repertoires could influence species regenerative capacity. To directly test that premise, we humanized the zebrafish p53 pathway by introducing regulatory and coding sequences of the human tumor suppressor ARF into the zebrafish genome. ARF was dormant during development, in uninjured adult fins, and during wound healing, but was highly expressed in the blastema during epimorphic fin regeneration after amputation. Regenerative, but not developmental signals resulted in binding of zebrafish E2f to the human ARF promoter and activated conserved ARF-dependent Tp53 functions. The context-dependent activation of ARF did not affect growth and development but inhibited regeneration, an unexpected distinct tumor suppressor response to regenerative versus developmental environments. The antagonistic pleiotropic characteristics of ARF as both tumor and regeneration suppressor imply that inducing epimorphic regeneration clinically would require modulation of ARF -p53 axis activation

PERCEPTIONS OF MOOSE-HUMAN CONFLICTS IN AN URBAN ENVIRONMENT

Urban expansion produces obvious and deleterious ecological effects on wildlife habitat. Land development plans continue to be approved in Prince George, British Columbia, both within and on proximate land that is occupied by moose (Alces alces). We surveyed 100 residents of Prince George to determine how they perceive potential conflicts with moose and compared those perceptions with available local data. The majority (~75%) indicated that there were <50 moose-human encounterswithin Prince George in any given year; however, 222 moose-related reports occurred from April 2007-March 2008. This discrepancy indicates that the public probably underestimates both the presence of moose and moose-human conflicts in Prince George. We did not find that outdoor enthusiasts were more knowledgeable than others about managing moose-human conflicts, suggesting that broad public education and awareness programs are warranted. Understanding how to respond to moose and developing a “Moose Aware” program were two suggested strategies to reduce conflict. The vast majority of residents (92%) enjoy moose and want moose to remain part of the Prince George environment; only 9% were in favour of euthanasia or sharp-shooting to resolve conflicts. Because 40% indicated that the best option was leaving moose alone, managers will need to develop more effective strategies to minimize and manage moose-human conflicts

PERCEPTIONS OF MOOSE-HUMAN CONFLICTS IN AN URBAN ENVIRONMENT

Urban expansion produces obvious and deleterious ecological effects on wildlife habitat. Land development plans continue to be approved in Prince George, British Columbia, both within and on proximate land that is occupied by moose (Alces alces). We surveyed 100 residents of Prince George to determine how they perceive potential conflicts with moose and compared those perceptions with available local data. The majority (~75%) indicated that there were <50 moose-human encounterswithin Prince George in any given year; however, 222 moose-related reports occurred from April 2007-March 2008. This discrepancy indicates that the public probably underestimates both the presence of moose and moose-human conflicts in Prince George. We did not find that outdoor enthusiasts were more knowledgeable than others about managing moose-human conflicts, suggesting that broad public education and awareness programs are warranted. Understanding how to respond to moose and developing a “Moose Aware” program were two suggested strategies to reduce conflict. The vast majority of residents (92%) enjoy moose and want moose to remain part of the Prince George environment; only 9% were in favour of euthanasia or sharp-shooting to resolve conflicts. Because 40% indicated that the best option was leaving moose alone, managers will need to develop more effective strategies to minimize and manage moose-human conflicts

TRACKING MOOSE- AND DEER-VEHICLE COLLISIONS USING GPS AND LANDMARK INVENTORY SYSTEMS IN BRITISH COLUMBIA

Vehicle collisions with moose (Alces alces) and deer (Odocoileus spp.) pose a serious threat to all motorists travelling highways traversing habitats of these two ungulates. In British Columbia, mitigation measures to reduce such collisions are based on spatially-accurate records of collisions involving moose and deer that are collected by the province’s highway maintenance contractors. To date, the British Columbia Ministry of Transportation and Infrastructure (BC MOTI) uses the paper-based Wildlife Accident Reporting System (WARS) established in 1978 to maintain carcass records. We compared carcass location data collected in 2010 to 2014 by BC MOTI using WARS to that collected by Northern Health Connections bus drivers using a newly developed GPS-based system (Otto® Wildlife device). In total, 6,929 carcasses (1,231 moose, 5,698 deer) were recorded using WARS and 474 (167 moose, 410 deer) using the Otto® Wildlife device. We compared data collected along 2,800 km on the same highways in the same seasons of the same years. We found more carcass locations were identified with the WARS method, but that in certain geographic regions, the Otto® Wildlife system identified several unique locations. We contend that more complete and finer-scale carcass location data is possible using a GPS-based system such as Otto® Wildlife, than currently collected solely with the paper-based WARS method

The effectiveness of decommissioning roadside mineral licks on reducing moose (Alces alces) activity near highways: implications for moose-vehicle collisions

Roadside mineral licks form when road salt used to de-ice highways in winter runs off road surfaces and accumulates in roadside ditches. Some ungulates are attracted to these roadside licks as they seek to satisfy their mineral requirements. Within the distribution of moose (Alces alces (Linnaeus, 1758)) in North America, motorists often encounter moose visiting roadside licks in mid-summer, with many jurisdictions reporting summer peaks in moose-vehicle collisions (MVCs) at these locations. Our research used camera traps to monitor moose visitation of 22 roadside locations (including roadside licks, roadside ponds, and dry roadsides) in central British Columbia, Canada from December 2009–July 2020, tested the efficacy of treatment (decommissioning) methods used to reduce moose visitation to roadside licks, and roughly estimated decommissioning costs. Moose visitation to roadside licks was greatest from May–July. As we hypothesized, untreated licks were visited more often by moose than decommissioned licks, roadside ponds (absent of road salt), and dry roadsides. Decommissioning roadside licks by replacing or mixing lick waters and soils with materials, such as riprap, cedar mulch, pine logs, or dog (Canis lupus familiaris (Linnaeus, 1758)) fur and human (Homo sapiens (Linnaeus, 1758)) hair, is an effective and inexpensive means of reducing moose visitations to roadside areas and should increase motorist safety where roadside licks are visited by moose.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Thumb CMC Joint Biomechanics: A Novel Device for Dynamic Splinting

Thumb carpometacarpal (CMC) joint arthritis is a major detriment to predominately post-menopausal women. The project team was asked to create a dynamic splint that combats the CMC joint arthritis, as most splints on the market do not treat the specific arthritis of the thumb. Along with the splint, the team developed a preliminary Finite Element Analysis (FEA) model of the CMC joint that was designed to analyze the stresses and strains that act on the joint during specific movements. The team performed material testing and tested the novel splint on non-arthritic patients to observe the efficacy of the splint on all parts of the hand. Based on the results, the team was able to revise their design as well as make future recommendations if the project were to be continued