Diantre, un homme dans les soins!: ou la vision de l'infirmière sur les rapports de séduction entre collègues

Contexte: Cette recherche initiale de type exploratoire s’inscrit dans le cadre de l’obtention d’un Bachelor of Sciences en Soins Infirmiers. Elle est élaborée en collaboration avec une autre étudiante de même niveau. But : Cette recherche a pour but d’explorer les rapports entre infirmiers et infirmières en milieu psychiatrique, et plus particulièrement, les relations de séduction. Elle vise à l’analyse des conséquences de ce type de relation sur la dynamique d’équipe, sur la prise en soins et le client et, enfin, sur l’infirmière et l’infirmier. Méthodologie : Six entretiens semi-directifs ont été menés auprès d’infirmières en psychiatrie. Ma collègue s’est intéressée au point de vue des infirmiers sur la même question. Après avoir retranscrit et classé les données recueillies, l’analyse a mis en évidence les liens entre les discours des participantes et le cadre théorique. La mise en commun des résultats des deux recherches ont permis de souligner le caractère similaire des propos. Résultats : Les rapports de séduction au sein de l’équipe soignante sont perçus comme anodins. Ils entraînent, dans la majorité des cas, une amélioration de la cohésion de groupe, une prise en soins de meilleure qualité et une augmentation de la motivation professionnelle de l’infirmière. A noter toutefois, que ces mêmes rapports peuvent avoir des conséquences néfastes dans de rares cas : perturbation de la dynamique d’équipe et diminution de la qualité des soins. Les infirmiers partagent ce point du vue. Recommandations : L’apport principal pour la pratique est la sensibilisation et le questionnement des différents professionnels sur leur propre séduction afin d’éviter toute dérive

B Mixing

The neutral $B$ mesons, $B^0$ and $B_s$, can oscillate between their particle and antiparticle states owing to flavor-changing weak interactions. In recent years, techniques to detect these oscillations as a function of the meson's decay time have been developed. In this article the physics of flavor oscillations is reviewed and theoretical predictions are summarized. The many observations that demonstrate the time-dependence of B^0-\B0bar oscillations are presented along with a combined measurement of its frequency, $\delta m_d$. The attempts to measure the $B_s$ oscillation frequency, both directly and indirectly, are then summarized. Finally, values for the CKM elements $|V_{td}|$ and $|V_{ts}/V_{td}|$ are extracted

Insect cell endocytosis of chikungunya virus adapted to Aedes albopictus, a mosquito recently introduced into southern France

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Intracellular assembly and budding of the Murine Leukemia Virus in infected cells

BACKGROUND: Murine Leukemia Virus (MLV) assembly has been long thought to occur exclusively at the plasma membrane. Current models of retroviral particle assembly describe the recruitment of the host vacuolar protein sorting machinery to the cell surface to induce the budding of new particles. Previous fluorescence microscopy study reported the vesicular traffic of the MLV components (Gag, Env and RNA). Here, electron microscopy (EM) associated with immunolabeling approaches were used to go deeply into the assembly of the "prototypic" MLV in chronically infected NIH3T3 cells. RESULTS: Beside the virus budding events seen at the cell surface of infected cells, we observed that intracellular budding events could also occur inside the intracellular vacuoles in which many VLPs accumulated. EM in situ hybridization and immunolabeling analyses confirmed that these latter were MLV particles. Similar intracellular particles were detected in cells expressing MLV Gag alone. Compartments containing the MLV particles were identified as late endosomes using Lamp1 endosomal/lysosomal marker and BSA-gold pulse-chase experiments. In addition, infectious activity was detected in lysates of infected cells. CONCLUSION: Altogether, our results showed that assembly of MLV could occur in part in intracellular compartments of infected murine cells and participate in the production of infectious viruses. These observations suggested that MLV budding could present similarities with the particular intracellular budding of HIV in infected macrophages

Fos Expression in Neurons of the Rat Vestibulo-Autonomic Pathway Activated by Sinusoidal Galvanic Vestibular Stimulation

The vestibular system sends projections to brainstem autonomic nuclei that modulate heart rate and blood pressure in response to changes in head and body position with regard to gravity. Consistent with this, binaural sinusoidally modulated galvanic vestibular stimulation (sGVS) in humans causes vasoconstriction in the legs, while low frequency (0.02–0.04 Hz) sGVS causes a rapid drop in heart rate and blood pressure in anesthetized rats. We have hypothesized that these responses occur through activation of vestibulo-sympathetic pathways. In the present study, c-Fos protein expression was examined in neurons of the vestibular nuclei and rostral ventrolateral medullary region (RVLM) that were activated by low frequency sGVS. We found c-Fos-labeled neurons in the spinal, medial, and superior vestibular nuclei (SpVN, MVN, and SVN, respectively) and the parasolitary nucleus. The highest density of c-Fos-positive vestibular nuclear neurons was observed in MVN, where immunolabeled cells were present throughout the rostro-caudal extent of the nucleus. c-Fos expression was concentrated in the parvocellular region and largely absent from magnocellular MVN. c-Fos-labeled cells were scattered throughout caudal SpVN, and the immunostained neurons in SVN were restricted to a discrete wedge-shaped area immediately lateral to the IVth ventricle. Immunofluorescence localization of c-Fos and glutamate revealed that approximately one third of the c-Fos-labeled vestibular neurons showed intense glutamate-like immunofluorescence, far in excess of the stain reflecting the metabolic pool of cytoplasmic glutamate. In the RVLM, which receives a direct projection from the vestibular nuclei and sends efferents to preganglionic sympathetic neurons in the spinal cord, we observed an approximately threefold increase in c-Fos labeling in the sGVS-activated rats. We conclude that localization of c-Fos protein following sGVS is a reliable marker for sGVS-activated neurons of the vestibulo-sympathetic pathway

Vasovagal Oscillations and Vasovagal Responses Produced by the Vestibulo-Sympathetic Reflex in the Rat

Sinusoidal galvanic vestibular stimulation (sGVS) induces oscillations in blood pressure (BP) and heart rate (HR), i.e., vasovagal oscillations, as well as transient decreases in BP and HR, i.e., vasovagal responses, in isoflurane-anesthetized rats. We determined the characteristics of the vasovagal oscillations, assessed their role in the generation of vasovagal responses, and determined whether they could be induced by monaural as well as by binaural sGVS and by oscillation in pitch. Wavelet analyses were used to determine the power distributions of the waveforms. Monaural and binaural sGVS and pitch generated vasovagal oscillations at the frequency and at twice the frequency of stimulation. Vasovagal oscillations and vasovagal responses were maximally induced at low stimulus frequencies (0.025–0.05 Hz). The oscillations were attenuated and the responses were rarely induced at higher stimulus frequencies. Vasovagal oscillations could occur without induction of vasovagal responses, but vasovagal responses were always associated with a vasovagal oscillation. We posit that the vasovagal oscillations originate in a low frequency band that, when appropriately activated by strong sympathetic stimulation, can generate vasovagal oscillations as a precursor for vasovagal responses and syncope. We further suggest that the activity responsible for the vasovagal oscillations arises in low frequency, otolith neurons with orientation vectors close to the vertical axis of the head. These neurons are likely to provide critical input to the vestibulo-sympathetic reflex to increase BP and HR upon changes in head position relative to gravity, and to contribute to the production of vasovagal oscillations and vasovagal responses and syncope when the baroreflex is inactivated

Cellular Uptake and Nuclear Delivery of Recombinant Adenovirus Penton Base

AbstractAn Ad2 capsid component, the penton base, expressed as recombinant protein, was found to be capable of affecting the entire entry pathway of adenovirion in HeLa cells, i.e., cell attachment, endocytosis, vesicular escape, intracytoplasmic movement, and translocation through the nuclear pore complex. Data with pentamerization-defective mutants suggested that none of these successive steps depended upon penton base pentamer status, indicating that the peptide domains responsible for these functions were carried by the monomer. Observations performed with wild-type (WT) and an integrin-binding-site double-mutant (K288E340) suggested that the penton base could enter the cell via an alternative, RGD- and LDV-independent, pathway. Of three mutants that were found to be defective in nuclear addressing in insect cells, only one, W165H, was also altered in nuclear transport in HeLa cells. The other two, W119H and RRR547EQQ, showed a WT pattern of nuclear localization in HeLa cells, suggesting that the region including tryptophan-119 and the basic signal at position 547 did not act as a nuclear localization signal in the human cell context. The integrity of cellular structures and the cytoskeleton seemed to be required for the vectorial movement and nuclear import of WT penton base, as suggested by experiments using permeabilized HeLa cells, isolated nuclear membranes, and cytoskeleton-targeted drugs