12 research outputs found
N-Benzyl-(2,5-dioxopyrrolidin-1-yl)propanamide (AS-1) with hybrid structure as a candidate for a broad-spectrum antiepileptic drug
In our recent studies, we identified compound N-benzyl-2-(2,5-dioxopyrrolidin-1-yl)propanamide (AS-1) as a broad-spectrum hybrid
anticonvulsant which showed potent protection across the most important animal acute seizure models such as the maximal electroshock
(MES) test, the subcutaneous pentylenetetrazole (s.c. PTZ) test, and the 6-Hz (32 mA) test in mice. Therefore, AS-1 may be
recognized as a candidate for new anticonvulsant effective in different types of human epilepsy with a favorable safety margin profile
determined in the rotarod test in mice. In the aim of further pharmacological evaluation of AS-1, in the current study, we examined its
activity in the 6-Hz (44 mA) test, which is known as the model of drug-resistant epilepsy. Furthermore, we determined also the
antiseizure activity in the kindling model of epilepsy induced by repeated injection of pentylenetetrazole (PTZ) in mice. As a result,
AS-1 revealed relatively potent protection in the 6-Hz (44 mA) test, as well as delayed the progression of kindling induced by repeated
injection of PTZ in mice at doses of 15 mg/kg, 30 mg/kg, and 60 mg/kg. Importantly, the isobolographic analysis showed that a
combination of AS-1 and valproic acid (VPA) at the fixed ratio of 1:1 displayed a supra-additive (synergistic) interaction against PTZinduced
seizures inmice. Thus, AS-1may be potentially used in an add-on therapy with VPA. Moreover, incubation of zebrafish larvae
with AS-1 substantially decreased the number, cumulative but not the mean duration of epileptiform-like events in electroencephalographic
assay. Finally, the in vitro ADME-Tox studies revealed that AS-1 is characterized by a very good permeability in the parallel
artificial membrane permeability assay test, excellent metabolic stability on human liver microsomes (HLMs), no significant influence
on CYP3A4/CYP2D6 activity, and moderate inhibition of CYP2C9 in a concentration of 10 M, as well as no hepatotoxic properties in
HepG2 cells (concentration of 10 M)
Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients.
microRNA (miRNA) belongs to the non-coding RNAs family responsible for the regulation of gene expression. Renalase is a protein composed of 342 amino acids, secreted by the kidneys and possibly plays an important role in the regulation of sympathetic tone and blood pressure. The aim of the present study was to investigate plasma renalase concentration, and explore the relationship between miRNA-146a-5p expression and plasma renalase levels in hemodialyzed patients.The study population comprised 55 subjects who succumbed to various cardiac events, 27 women and 28 men, aged 65-70 years. The total RNA including miRNA fraction was isolated using QiagenmiRNEasy Serum/Plasma kit according to the manufacturer's protocol. The isolated miRNAs were analyzed using a quantitative polymerase chain reaction (qRT-PCR) technique. The plasma renalase levels were measured using a commercial ELISA kit.In the group of patients with high levels of renalase, higher miRNA-146a expression was found, compared with those with low concentration of renalase. Patients with simultaneous low miRNA-146a expression and high level of renalase were confirmed to deliver a significantly longer survival time compared with other patients.miRNA-146a and plasma renalase levels were estimated as independent prognostic factors of hemodialyzed patients' survival time. Patients with low miRNA-146a expression demonstrated a significantly longer survival time in contrast to the patients with a high expression level of miRNA-146a. Moreover, a significantly longer survival time was found in patients with high renalase activity compared with patients with low activity of the enzyme
Shape-Morphing of an Artificial Protein Cage with Unusual Geometry Induced by a Single Amino Acid Change
Artificial protein cages are constructed from multiple protein subunits. The interaction between the subunits, notably the angle formed between them, controls the geometry of the resulting cage. Here, using the artificial protein cage, “TRAP-cage”, we show that a simple alteration in the position of a single amino acid responsible for Au(I)-mediated subunit–subunit interactions in the constituent ring-shaped building blocks results in a more acute dihedral angle between them. In turn, this causes a dramatic shift in the structure from a 24-ring cage with an octahedral symmetry to a 20-ring cage with a C2 symmetry. This symmetry change is accompanied by a decrease in the number of Au(I)-mediated bonds between cysteines and a concomitant change in biophysical properties of the cage
Probability of overall survival change depending on simultaneous analysis of renalase concentration and miRNA-146a expression level in hemodialyzed patients.
<p>Patients with simultaneous high concentration of renalase and low miRNA-146a expression demonstrate significantly higher OS compared with other studied patients (OS—77 vs. 39 months; p = 0.018; HR = 2.01).</p
Probability of overall survival change depending on miRNA-146a expression level in the studied group.
<p>Low miRNA-146a expression level is a favorable prognostic factor of patients’ survival compared with patients with high expression of this miRNA (OS—69 vs. 56 months; p = 0.430; HR = 1.728).</p
Baseline characteristics in long-term dialysis patients.
<p>Baseline characteristics in long-term dialysis patients.</p
Raw Data Plos One.docx
Raw data used for the preparation of a manuscript entitled "Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients", which is published in the Plos One journal <br