Validation Methods Research for Fault-Tolerant Avionics and Control Systems: Working Group Meeting, 2

The validation process comprises the activities required to insure the agreement of system realization with system specification. A preliminary validation methodology for fault tolerant systems documented. A general framework for a validation methodology is presented along with a set of specific tasks intended for the validation of two specimen system, SIFT and FTMP. Two major areas of research are identified. First, are those activities required to support the ongoing development of the validation process itself, and second, are those activities required to support the design, development, and understanding of fault tolerant systems

Simultaneous Representation on Adverse Interests: Suing One Client on Behalf of Another - In re Dresser Industries, Inc.

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Effect of humidity on transonic flow

An experimental investigation of the effects of humidity-induced condensation on shock/boundary-layer interaction has been conducted in a transonic wind-tunnel test. The test geometry considered was a wall-mounted bump model inserted in the test section of the wind tunnel. The formation of a λ-shape condensation shock wave was shown from schlieren visualization and resulted in a forward movement of the shock wave, reduced shock wave strength, and reduced separation. Empirical correlations of the shock wave strength and humidity/dew point temperature were established. For humidity levels below 0.15 or a dew point temperature of 268 K, the effect of humidity was negligible. The unsteady pressure measurements showed that if a condensation shock wave formed and interacted with a main shock wave, the flow becomes unsteady with periodic flow oscillations occurring at 720 Hz

A feasibility study for a remote laser water turbidity meter

A technique to remotely determine the attenuation coefficient (alpha) of the water was investigated. The backscatter energy (theta = 180 deg) of a pulse laser (lambda = 440 - 660 nm) was found directly related to the water turbidity. The greatest sensitivity was found to exist at 440 nm. For waters whose turbidity was adjusted using Chesapeake Bay sediment, the sensitivity in determining alpha at 440 nm was found to be approximately 5 - 10%. A correlation was also found to exist between the water depth (time) at which the peak backscatter occurs and alpha

The Friedheim\u27s of Rock Hill, South Carolina - Accession 715 #67

The Friedheim\u27s of Rock Hill, South Carolina relates the history of the family through narrative, stories, anecdotes and photographs. There is a outline of the family connections at the back of the booklet. The Friedheim Brothers operated a department store in Rock Hill in 1866. The store closed in 1965. The author, C.H. Albright married one of Arnold Friedheim\u27s granddaughters.https://digitalcommons.winthrop.edu/manuscriptcollection_findingaids/2603/thumbnail.jp

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors

Detergents enable the purification of membrane proteins and are indispensable reagents instructural biology. Even though a large variety of detergents have been developed in the lastcentury, the challenge remains to identify guidelines that allowfine-tuning of detergents forindividual applications in membrane protein research. Addressing this challenge, here weintroduce the family of oligoglycerol detergents (OGDs). Native mass spectrometry (MS)reveals that the modular OGD architecture offers the ability to control protein purificationand to preserve interactions with native membrane lipids during purification. In addition to abroad range of bacterial membrane proteins, OGDs also enable the purification and analysisof a functional G-protein coupled receptor (GPCR). Moreover, given the modular design ofthese detergents, we anticipatefine-tuning of their properties for specific applications instructural biology. Seen from a broader perspective, this represents a significant advance forthe investigation of membrane proteins and their interactions with lipids

Subcellular distribution of carbonic anhydrase in Solanum tuberosum L. leaves

The intracellular compartmentation of carbonic anhydrase (CA; EC 4.2.1.1), an enzyme that catalyses the reversible hydration of CO2 to bicarbonate, has been investigated in potato (#Solanum tuberosum$ L.) leaves. Although enzyme activity was mainly located in chloroplasts (87% of total cellular activity), significant activity (13%) was also found in the cytosol. The corresponding CA isoforms were purified either from chloroplasts or crude leaf extracts, respectively. The cytosolic isoenzyme has a molecular mass of 255 000 and is composed of eight identical subunits with an estimated Mr of 30 000. The chloroplastic isoenzyme (Mr 220 000) is also an octamer composed of two different subunits with Mr estimated at 27 000 and 27 500, respectively. The N-terminal amino acid sequences of both chloroplastic CA subunits demonstrated that they were identical except that the Mr-27 000 subunit was three amino acids shorter than that of the Mr-27 500 subunit. Cytosolic and chloroplastic CA isoenzymes were found to be similarly inhibited by monovalent anions (Cl-, I-, N3- and NO3-) and by sulfonamides (ethoxyzolamide and acetozolamide). Both CA isoforms were found to be dependent on a reducing agent such as cysteine or dithiothreitol in order to retain the catalytic activity, but 2-mercaptoethanol was found to be a potent inhibitor. A polyclonal antibody directed against a synthetic peptide corresponding to the N-terminal amino acid sequence of the chloroplastic CA monomers also recognized the cytosolic CA isoform. This antibody was used for immunocytolocalization experiments which confirmed the intracellular compartmentation of CA : within chloroplasts, CA is restricted to the stroma and appears randomly distributed in the cytosol. (Résumé d'auteur