The Grizzly, March 21, 2002

Wynton Marsalis Brings Down the House at Ursinus • The True History of St. Patrick\u27s Day • Music Series set for Berman Museum • Ursinus Women\u27s History Month Dedicated to Late Professor • Meistersingers to Perform at Ursinus College • Berman Museum of Art to Feature Hans Moller Retrospective • SERV and Campus Safety to the Rescue • The New Fad Drug that Might be a Thinly Veiled Disaster • Girls Lacrosse Starts out Strong after a Productive Southern Swinghttps://digitalcommons.ursinus.edu/grizzlynews/1510/thumbnail.jp

Transcriptomic comparison of invasive bigheaded carps (\u3ci\u3eHypophthalmichthys nobilis\u3c/i\u3e and \u3ci\u3eHypophthalmichthys molitrix\u3c/i\u3e) and their hybrids

Bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix), collectively called bigheaded carps, are invasive species in the Mississippi River Basin (MRB). Interspecific hybridization between bigheaded carps has been considered rare within their native rivers in China; however, it is prevalent in the MRB. We conducted de novo transcriptome analysis of pure and hybrid bigheaded carps and obtained 40,759 to 51,706 transcripts for pure, F1 hybrid, and backcross bigheaded carps. The search against protein databases resulted in 20,336–28,133 annotated transcripts (over 50% of the transcriptome) with over 13,000 transcripts mapped to 23 Gene Ontology biological processes and 127 KEGG metabolic pathways. More transcripts were detected in silver carp than in bighead carp; however, comparable numbers of transcripts were annotated. Transcriptomic variation detected between two F1 hybrids may indicate a potential loss of fitness in hybrids. The neighbor-joining distance tree constructed using over 2,500 one-to-one orthologous sequences suggests transcriptomes could be used to infer the history of introgression and hybridization. Moreover, we detected 24,792 candidate SNPs that can be used to identify different species. The transcriptomes, orthologous sequences, and candidate SNPs obtained in this study should provide further knowledge of interspecific hybridization and introgression

The complete mitochondrial genome of the shoal chub, Macrhybopsis hyostoma

The complete mitochondrial genome of the shoal chub (Macrhybopsis hyostoma) was determined to be 16,899 bp and contained 22 tRNA genes, 2 rRNA genes and 1 control region. The whole genome base composition was 30.5% A, 28.5% T, 24.9% C and 16.1 G. This complete mitochondrial genome provides essential molecular markers for resolving phylogeny and future conservation efforts

The complete mitochondrial genome of the silver chub, Macrhybopsis storeriana

The complete mitochondrial genome of the silver chub M. storeriana was determined to be 16,709 bp and contained 22 tRNA genes, 2 rRNA genes and one control region. The whole genome base composition was 30.3% A, 28% T, 25.5% C and 16.2 G. This complete mitochondrial genome provides essential molecular markers for resolving phylogeny and aiding future conservation efforts

Transcriptomic variation of hepatopancreas reveals the energy metabolism and biological processes associated with molting in Chinese mitten crab, Eriocheir sinensis

Molting is a critical developmental process for crustaceans, yet the underlying molecular mechanism is unknown. In this study, we used RNA-Seq to investigate transcriptomic profiles of the hepatopancreas and identified differentially expressed genes at four molting stages of Chinese mitten crab (Eriocheir sinensis). A total of 97,398 transcripts were assembled, with 31,900 transcripts annotated. Transcriptomic comparison revealed 1,189 genes differentially expressed amongst different molting stages. We observed a pattern associated with energy metabolism and physiological responses during a molting cycle. In specific, differentially expressed genes enriched in postmolt were linked to energy consumption whereas genes enriched in intermolt were related to carbohydrates, lipids metabolic and biosynthetic processes. In premolt, a preparation stage for upcoming molting and energy consumption, highly expressed genes were enriched in response to steroid hormone stimulus and immune system development. The expression profiles of twelve functional genes detected via RNA-Seq were corroborated through real-time RT-PCR assay. Together, our results, including assembled transcriptomes, annotated functional elements and enriched differentially expressed genes amongst different molting stages, provide novel insights into the functions of the hepatopancreas in energy metabolism and biological processes pertaining to molting in crustaceans

The Grizzly, April 25, 2002

Pesta Appeals College\u27s Action • O\u27Flaherty Speaks to Ursinus about Human Rights • Diverse and Abundant Research Continues • Sex for Sale: What Drives the Porn Industry? • Final Exam Schedule • Jon Volkmer Named Montgomery County Poet Laureate • Greek Week Change Bad: An Opinion • Commencement and Baccalaureate Speakers Announced • Hockey Intramurals • Family or no Family: That is the Question for Many Career Womenhttps://digitalcommons.ursinus.edu/grizzlynews/1515/thumbnail.jp

Resolving the genetic paradox of invasions: Preadapted genomes and postintroduction hybridization of bigheaded carps in the Mississippi River Basin

The genetic paradox of biological invasions is complex and multifaceted. In particular, the relative role of disparate propagule sources and genetic adaptation through postintroduction hybridization has remained largely unexplored. To add resolution to this paradox, we investigate the genetic architecture responsible for the invasion of two invasive Asian carp species, bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix) (bigheaded carps) that experience extensive hybridization in the Mississippi River Basin (MRB). We sequenced the genomes of bighead and silver carps (~1.08G bp and ~1.15G bp, respectively) and their hybrids collected from the MRB. We found moderate‐to‐high heterozygosity in bighead (0.0021) and silver (0.0036) carps, detected significantly higher dN/dS ratios of single‐copy orthologous genes in bigheaded carps versus 10 other species of fish, and identified genes in both species potentially associated with environmental adaptation and other invasion‐related traits. Additionally, we observed a high genomic similarity (96.3% in all syntenic blocks) between bighead and silver carps and over 90% embryonic viability in their experimentally induced hybrids. Our results suggest intrinsic genomic features of bigheaded carps, likely associated with life history traits that presumably evolved within their native ranges, might have facilitated their initial establishment of invasion, whereas ex-situ interspecific hybridization between the carps might have promoted their range expansion. This study reveals an alternative mechanism that could resolve one of the genetic paradoxes in biological invasions and provides invaluable genomic resources for applied research involving bigheaded carps

The Grizzly, October 25, 2001

Food and Fun at Family Day 2001 • UC Field Hockey Players React to Move from Division I to Division III • Phi Psi Promotes Breast Cancer Awareness Month with Pink Ribbon Fundraiser • Fall Fun and Friendship with UC Best Buddies • Truth About Dr. Fritz • Gay Straight Alliance Walks for AIDS Fund • First Year Student Coordinator Ed Gildea Helps Students Work to their Fullest Potential • Take Advantage of the Upcoming Internship Fair • Students Learn the Truth about Drinking • Opinions: Athletic Scholarships at Ursinus; Listen up Insurance Companies: Give me my Birth Control! • Stunned in Awe: It\u27s all in the Name • Birth Control Basics • Bears Take Down Wooster • UC Field Hockey Takes on The Capital • Bears Volleyball Falls into a Slump after Beating DeSales • Women\u27s Soccer Builds up Momentum to Beat Villa Julie • Veteran\u27s Day for UC Men\u27s Soccer • Penderghest and Dougherty Run to Bear Pack Victory! • Splish, Splash, UC is Back in a Flash • Outlook for 2002 Lacrosse • Ursinus Cheerleadinghttps://digitalcommons.ursinus.edu/grizzlynews/1498/thumbnail.jp

The Grizzly, April 18, 2002

From the B-52\u27s to the Beach Boys: Airband was a Huge Success • ProTheatre Stages History with Twilight: Los Angeles this Weekend • Students Rave About the Pat McGee Band in Concert • Adolph Reed to Deliver Pancoast Lecture • Fitness Week a Fitness Blast • Male Birth Control: Latest Medical Breakthrough? • Opinions: Greek Portrayal in Movies Demeaning, not True!; Parking Policy not Fun for Some Residents • Y100\u27s Caseyboy and his Crazy Stunts • The Letters and the Cane • Comparative Pricing Report: Self Tanners • Skin Cancer is not Your Friend • UC Women\u27s Rugby Making a Breakthrough in Women\u27s Sports • Workman\u27s Workouts Paying Off for Field Hockey • Baseball Team Winds Down Season Looking for Top Spot in Centennial Conference Playoffs • Erin Fitzgerald Named Centennial Conference Women\u27s Lacrosse Player of the Week • Dougherty Qualifies in 5000m for NCAA Championships • Another Flawless Season for Women\u27s Lacrosse to Lead Team to Second Consecutive Championship Title • UC Men\u27s LAX Stars: What are they up to Now? • Win, Loss, and Postponement for UC Softball as Season Winds Downhttps://digitalcommons.ursinus.edu/grizzlynews/1514/thumbnail.jp