Isolamento de Candida spp. de estomatite relacionada à prótese no Pará, Brasil.

The aim of this study was to isolate and identify Candida species from the oral cavity of den-ture wearers with denture-related stomatitis who were attended at the University Federalof Pará (Belém City, Pará State, Brazil). A total of 36 denture wearers with denture-relatedstomatitis were included, and type I (50%), type II (33%) and type III (17%) stomatitis wereobserved. Candida spp. were isolated from 89% of the cases and included five differentCandida species. C. albicans was the most frequently recovered species (78% of the cases),followed by C. famata and C. tropicalis. We observed a significant association between Candidaspecies isolation and unsatisfactory denture condition (p = 0.0017). Our results demonstratedthe highly frequency of Candida species isolation in denture wearers with denture-relatedstomatitis and showed the relationship between these species and poor denture maintence.O objetivo deste estudo foi isolar e identificar espécies de Candida da cavidade oral de usuários com estomatite relacionada à dentadura, atendidos na Universidade Federal do Pará (Cidade de Belém, Pará, Brasil). Foram incluídos 36 usuários de protese com estomatite relacionada à prótese, e foram observadas estomatite tipo I (50%), tipo II (33%) e tipo III (17%). Candida spp. foram isolados em 89% dos casos e incluíram cinco espécies diferentes de Candida. C. albicans, frequentemente recuperada (78% dos casos), seguida por C. famata e C. tropicalis. Observamos uma associação significativa entre o isolamento da espécie Candidas e a condição insatisfatória da prótese (p = 0,0017). Nossos resultados demonstraram a alta frequência de isolamento de espécies de Candida em usuários de próteses com estomatite relacionada à prótese e mostraram a relação entre essas espécies e má manutenção da prótese

Isolation of Candida spp. from denture-related stomatitis in Pará, Brazil

The preparation of this manuscript was supported by the Postgraduate Program in Biology ofInfectious and Parasitic Agents, Federal University of Pará (Edital PAPQ/2013-UFPA).Federal University of Pará. Science Health Institute Belém. Odontology College. Belém, PA, Brazil / Federal University of Pará. Science Biologic Institute. Postgraduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Federal University of Pará. Science Health Institute Belém. Odontology College. Belém, PA, BrazilFederal University of Pará. Science Biologic Institute. Postgraduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Federal University of Pará. Science Health Institute Belém. Odontology College. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Micologia. Ananindeua, PA, Brasil.The aim of this study was to isolate and identify Candida species from the oral cavity of denture wearers with denture-related stomatitis who were attended at the University Federal of Pará (Belém City, Pará State, Brazil). A total of 36 denture wearers with denture-related stomatitis were included, and type I (50%), type II (33%) and type III (17%) stomatitis were observed. Candida spp. were isolated from 89% of the cases and included five different Candida species. C. albicans was the most frequently recovered species (78% of the cases), followed by C. famata and C. tropicalis. We observed a significant association between Candida species isolation and unsatisfactory denture condition (p = 0.0017). Our results demonstrated the highly frequency of Candida species isolation in denture wearers with denture-related stomatitis and showed the relationship between these species and poor denture maintenance

Relationship among local and functional factors in the development of denture stomatitis in denture wearers in northern Brazil

OBJECTIVE: The aim of this study was to evaluate the relationship among functional and qualitative factors in the development of denture stomatitis (DS) (according to Newton's classification) in acrylic-based denture wearers residents from northern Brazil.MATERIAL AND METHOD: A total of 99 patients who wore partial or total acrylic resin-based upper dentures were included in this study. The subjects completed an epidemiological data form that includes the patient's gender, age, local factors (hygiene habits, remove denture to sleep, use of mouthwash, present condition of the denture, age of the denture) and functional factors (vertical dimension at rest, vertical dimension of occlusion, occlusion, retention, and static and dynamic stability). To detect yeasts, samples were collected from the inner surface of the dentures and from the palatal mucosa in contact with it. Subsequently, the samples were cultured on Sabouraud dextrose agar, observing macro and microscopic characteristics.RESULT: In the present study, we did not find any significant relationship between the gender and disease onset. Based on the Newton classification, 36.3% of the patients presented with DS and 89.0% were colonized by yeasts; of these subjects, 50% had type I lesions, 33.3% had type II lesions, and 16.6% had type III lesions. All of the qualitative and local factors, except the use of mouthwash, were clinically relevant to the development of disease.CONCLUSION: Denture stomatitis in denture users in northern Brazil was multifactorial, involving local, functional and microbiological factors

Relationship among local and functional factors in the development of denture stomatitis in denture wearers in northern Brazil

OBJECTIVE: The aim of this study was to evaluate the relationship among functional and qualitative factors in the development of denture stomatitis (DS) (according to Newton's classification) in acrylic-based denture wearers residents from northern Brazil.MATERIAL AND METHOD: A total of 99 patients who wore partial or total acrylic resin-based upper dentures were included in this study. The subjects completed an epidemiological data form that includes the patient's gender, age, local factors (hygiene habits, remove denture to sleep, use of mouthwash, present condition of the denture, age of the denture) and functional factors (vertical dimension at rest, vertical dimension of occlusion, occlusion, retention, and static and dynamic stability). To detect yeasts, samples were collected from the inner surface of the dentures and from the palatal mucosa in contact with it. Subsequently, the samples were cultured on Sabouraud dextrose agar, observing macro and microscopic characteristics.RESULT: In the present study, we did not find any significant relationship between the gender and disease onset. Based on the Newton classification, 36.3% of the patients presented with DS and 89.0% were colonized by yeasts; of these subjects, 50% had type I lesions, 33.3% had type II lesions, and 16.6% had type III lesions. All of the qualitative and local factors, except the use of mouthwash, were clinically relevant to the development of disease.CONCLUSION: Denture stomatitis in denture users in northern Brazil was multifactorial, involving local, functional and microbiological factors

El efecto del aceite esencial de Rosmarinus officinalis sobre la formación del tubo germinativo en Candida dubliniensis aislada de usuarios de dentadura

Universidade Federal do Pará. Instituto de Ciências Biológicas. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brasil / Universidade Federal do Pará. Instituto de Ciências da Saúde. Faculdade de Odontologia. Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências da Saúde. Faculdade de Odontologia. Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências da Saúde. Faculdade de Odontologia. Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brasil.Museu Paraense Emílio Goeldi. Laboratório de Botânica. Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Laboratório de Extração. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Candida dubliniensis is an emerging pathogenic yeast that is closely related to C. albicans and colonizes or infects the oral cavities of denture users. Similar to C. albicans, C. dubliniensis produces germ tubes as a major virulence factor. Due to the scarcity of data demonstrating that natural products have anti-C. dubliniensis activity, the objective of this study was to evaluate the effect of an essential oil from Rosmarinus officinalis on C. dubliniensis germ tube formation. Six isolates of C. dubliniensis were recovered from the oral cavities (n = 5/denture base and n = 1/palatal mucosa) of six denture users either with (n = 4) or without (n = 2) stomatitis. Germ tube formation was induced in Sabouraud dextrose broth containing 10% fetal bovine serum in the presence (test) or absence (control) of 4% R. officinalis essential oil. The complete inhibition of germ tube formation for all of the tested isolates of C. dubliniensis was observed (p = 0.004). Our results demonstrate the potent anti-C. dubliniensis effect of R. officinalis essential oil, indicating the need to further investigate the pharmaceutical use of R. officinalis essential oil, particularly as it impacts the colonization of denture resin and stomatitis.Candida dubliniensis é uma levedura patogênica emergente, muito semelhante à C. albicans, que coloniza ou infecta as cavidades bucais de usuários de dentaduras. Similar à C. albicans, C. dubliniensis produz tubos germinativos com fator de virulência maior. Devido à escassez de dados, demonstrando que produtos naturais têm atividade anti-C. dubliniensis, o objetivo deste estudo foi avaliar o efeito de um óleo essencial de Rosmarinus officinalis sobre a formação de tubo germinativo em C. dubliniensis. Seis isolados de C. dubliniensis foram coletados de cavidades orais (n = 5/ base da dentadura e n = 1/mucosa palatal) de seis usuários de dentaduras com (n = 4) ou sem (n = 2) estomatite. A formação do tubo germinativo foi induzida em caldo Sabouraud dextrose, contendo soro fetal bovino a 10% na presença (teste) ou ausência (controle) de óleo essencial de R. officinalis a 4%. Observou-se a completa inibição da formação do tubo germinativo de todos os isolados de C. dubliniensis (p = 0,004). Nossos resultados demonstraram o potente efeito anti-C. dubliniensis do óleo essencial R. officinalis, indicando a necessidade maiores investigações sobre o uso farmacêutico do óleo essencial R. officinalis, particularmente o seu impacto sobre a colonização da resina para dentaduras e a estomatite

Actividad antifúngica de Rosmarinus officinalis Linn. aceite esencial contra Candida albicans, Candida dubliniensis, Candida parapsilosis y Candida krusei

Universidade Federal do Pará. Instituto de Ciências Biológicas. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brasil / Universidade Federal do Pará. Instituto de Ciências da Saúde. Faculdade de Odontologia. Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências da Saúde. Faculdade de Odontologia. Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências da Saúde. Faculdade de Odontologia. Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Programa de Pós-graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brasil.Museu Paraense Emílio Goeldi. Laboratório de Botânica. Belém, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Laboratório de Extração. Belém, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.The antifungal and anti-germ tube formation activity of Rosmarinus officinalis Linn. essential oil was tested against four Candida strains (C. albicans, C. dubliniensis, C. parapsilosis and C. krusei). Inhibition halo sizes and minimal inhibitory concentrations (MIC) were obtained using radial diffusion and micro dilution tests, respectively. The minimal fungicidal concentration (MFC) was obtained from the MIC assay. Additionally, the effect of the essential oil on germ tube formation in C. albicans and C. dubliniensis was evaluated. The MIC50 ranged from 0.5% to 2%, while the MFC ranged from 1% to 2%. We observed total inhibition of germ tube formation in C. albicans and C. dubliniensis. The R. officinalis Linn. essential oil displayed powerful inhibitory and fungicidal activity against specific Candida strains.A atividade de formação do tubo antifúngico e antigerme do óleo essencial de Rosmarinus officinalis Linn. foi testado contra quatro cepas de Candida (C. albicans, C. dubliniensis, C. parapsilosis e C. krusei). Halos de inibição e concentração inibitória mínima (MIC) foram obtidos utilizando os testes de difusão radial e de microdiluição, respectivamente. A concentração fungicida mínima (MFC) foi obtida por meio de ensaio da MIC. Além disso, o efeito do óleo essencial na formação do tubo germinativo de C. albicans e C. dubliniensi foi avaliado. A MIC50 variou de 0,5% a 2%, enquanto a MFC variou de 1% a 2%. Observou-se a inibição total do crescimento do tubo germinativo em C. albicans e C. dubliniensis. O óleo essencial de R. officinalis Linn. demonstrou potente atividade inibitória e fungicida contra cepas específicas de Candida

Effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers

Introduction The aim of this study was to investigate the effects of Rosmarinus officinalis essential oil on germ tube formation by Candida albicans isolated from denture wearers. Methods Ten C. albicans isolates recovered from denture wearers were tested using 10% fetal bovine serum with or without 4% R. officinalis essential oil. Results The essential oil from R. officinalis completely inhibited germ tube formation in the investigated C. albicans isolates. Conclusions The results demonstrate that the essential oil of R. officinalis modulates C. albicans pathogenicity through its primary virulence factor (i.e., germ tube formation was suppressed)