22 research outputs found
Controlling the denaturation and aggregation of whey proteins using Îș-casein and caseinomacropeptide
Whey proteins ingredients are extensively used in a variety of product formulations
such as dairy beverages, infant formula and sport nutritional beverages, due to their
nutritional and functional properties. Dairy protein-containing beverages are
thermally processed, typically to ensure microbiological safety. However, whey
proteins denature and aggregate at temperatures greater than 60°C, which can lead to
fouling of industrial equipment and/or uncontrolled gelation, depending on
formulation and heating conditions. The presence of caseins has been previously
reported to limit the extent of aggregation of whey proteins. The objective of this
study was to investigate the effect of Îș-casein and caseinomacropeptide (CMP) on
the denaturation and aggregation of whey proteins, with a view to developing
practical strategies for controlling whey protein denaturation and aggregation for
ingredient applications. This study demonstrated that both Îș-casein and CMP have
the ability to improve the heat stability of whey proteins. The inclusion of Îș-casein
reduced the size of the aggregates of whey protein after a first heat treatment (90°C
for 25 min at pH 7.2) and enhanced their solubility during subsequent heating (90°C
for 1 h at pH 7.2). The presence of CMP during heating increased the temperatures
of denaturation and gelation of whey proteins and prevented the formation of solid
whey protein gels when combined with a low heating rate. The presence of CMP
also resulted in a lower turbidity of whey protein solutions after heating and an
enhanced solubility of whey protein aggregates. The effect of glycosylation of CMP
on the denaturation and aggregation of whey proteins was pH-dependent; a transition
occurred at pH 6, below which the glycosylation of CMP reduced its stabilizing
properties. This thesis provides new insights into the interactions of whey proteins
with Îș-casein and CMP, with potential for novel applications in improving the
heat-stability and solubility of whey proteins. The outcomes of this study have
applications for the manufacture of clear, heat-stable beverages containing whey
proteins
Isolation and characterisation of Îș-casein/whey protein particles from heated milk protein concentrate and role of Îș-casein in whey protein aggregation
peer-reviewedMilk protein concentrate (79% protein) reconstituted at 13.5% (w/v) protein was heated (90 °C, 25 min, pH 7.2) with or without added calcium chloride. After fractionation of the casein and whey protein aggregates by fast protein liquid chromatography, the heat stability (90 °C, up to 1 h) of the fractions (0.25%, w/v, protein) was assessed. The heat-induced aggregates were composed of whey protein and casein, in whey protein:casein ratios ranging from 1:0.5 to 1:9. The heat stability was positively correlated with the casein concentration in the samples. The samples containing the highest proportion of caseins were the most heat-stable, and close to 100% (w/w) of the aggregates were recovered post-heat treatment in the supernatant of such samples (centrifugation for 30 min at 10,000 Ă g). Îș-Casein appeared to act as a chaperone controlling the aggregation of whey proteins, and this effect was stronger in the presence of αS- and ÎČ-casein.This work was supported by Dairy Levy Research Trust (project MDDT6261 âProPartâ). S. J. Gaspard was funded under the Teagasc Walsh Fellowship Scheme (reference number 2012211
Influence of Chaperone-Like Activity of Caseinomacropeptide on the Gelation Behaviour of Whey Proteins at pH 6.4 and 7.2
The effect of caseinomacropeptide (CMP) on the heat-induced denaturation and gelation of whey proteins (2.5â10%, w/v) at pH 6.4 and 7.2, at a whey protein:CMP ratio of 1:0.9 (w/w), was investigated using differential scanning calorimetry (DSC), oscillatory rheology (90 °C for 20 min) and confocal microscopy. Greater frequency-dependence in the presence of CMP suggested that the repulsive interactions between CMP and the whey proteins affected the network generated by the non-heated whey protein samples. At pH 6.4 or 7.2, CMP increased the temperature of denaturation of ÎČ-lactoglobulin by up to 3 °C and increased the gelation temperature by up to 7 °C. The inclusion of CMP strongly affected the structure of the heat-induced whey protein gels, resulting in a finer stranded structure at pH 6.4 and 7.2. The presence of CMP combined with a lower heating rate (2 °C/min) prevented the formation of a solid gel of whey proteins after heating for 20 min at 90 °C and at pH 7.2. These results show the potential of CMP for control of whey protein denaturation and gelation
Influence of desialylation of caseinomacropeptide on the denaturation and aggregation of whey proteins
peer-reviewedABSTRACT The effect of the addition of caseinomacropeptide (CMP) or desialylated CMP on the heat-induced denaturation and aggregation of whey proteins was investigated in the pH range 3 to 7 after heating at 80°C for 30 min. The rate and temperature of denaturation, the extent of aggregation, and the changes in secondary structure of the whey proteins heated in presence of CMP or desialylated CMP were measured. The sialic acid bound to CMP favored the denaturation and aggregation of whey proteins when the whey proteins were oppositely charged to CMP at pH 4. A transition occurred at pH 6, below which the removal of sialic acid enhanced the stabilizing properties of CMP against the denaturation and aggregation of the whey proteins. At pH >6, the interactions between desialylated CMP and the whey proteins led to more extensive denaturation and aggregation. Sialic acid bound to CMP influenced the denaturation and aggregation behavior of whey proteins in a pH-dependent manner, and this should be considered in future studies on the heat stability of such systems containing CMP
Detection chain and electronic readout of the QUBIC instrument
The Q and U Bolometric Interferometer for Cosmology (QUBIC) Technical Demonstrator (TD) aiming to shows the feasibility of the combination of interferometry and bolometric detection. The electronic readout system is based on an array of 128 NbSi Transition Edge Sensors cooled at 350mK readout with 128 SQUIDs at 1K controlled and amplified by an Application Specific Integrated Circuit at 40K. This readout design allows a 128:1 Time Domain Multiplexing. We report the design and the performance of the detection chain in this paper. The technological demonstrator unwent a campaign of test in the lab. Evaluation of the QUBIC bolometers and readout electronics includes the measurement of I-V curves, time constant and the Noise Equivalent Power. Currently the mean Noise Equivalent Power is ~ 2 x 10â»ÂčⶠW/âHz
Detection chain and electronic readout of the QUBIC instrument
The Q and U Bolometric Interferometer for Cosmology (QUBIC) Technical Demonstrator (TD) aiming to shows the feasibility of the combination of interferometry and bolometric detection. The electronic readout system is based on an array of 128 NbSi Transition Edge Sensors cooled at 350mK readout with 128 SQUIDs at 1K controlled and amplified by an Application Specific Integrated Circuit at 40K. This readout design allows a 128:1 Time Domain Multiplexing. We report the design and the performance of the detection chain in this paper. The technological demonstrator unwent a campaign of test in the lab. Evaluation of the QUBIC bolometers and readout electronics includes the measurement of I-V curves, time constant and the Noise Equivalent Power. Currently the mean Noise Equivalent Power is ~ 2 x 10â»ÂčⶠW/âHz
Ge/Si and Si isotopes in thermal waters and rivers draining the Yellowstone Plateau Volcanic Field, USA
Yellowstone Plateau Volcanic Field, USA: Ge concentrations measured by inductively coupled plasma mass spectrometry (ICP-MS), Si concentrations measured by inductively coupled plasma optical emission spectrometry (ICP-OES), Ge/Si ratio, Si isotope compositions (d30Si and standard deviation SD) measured by Multicollector ICP-MS, concentrations in Ca, Na, Mg, K measured by ICP-OES, and concentrations in SO4 and Cl measured by ion chromatography in thermal waters, major rivers draining the Yellowstone Plateau Volcanic Field, and creeks flowing into Yellowstone Lake
The Holocene silicon biogeochemistry of Yellowstone Lake, USA
Silicon (Si) is an essential macronutrient for diatoms, an important component of lacustrine primary productivity that represents a link between the carbon and silicon cycles. Reconstructions of lake silicon cycling thus provide an underexploited window onto lake and catchment biogeochemistry. Silicon isotope geochemistry has potential to provide these reconstructions, given the competing source and process controls can be deconvolved. The silicarich volcanic and hydrothermal systems in Yellowstone National Park are a great source of dissolved silicon into Yellowstone Lake, a system with high silicon, and thus carbon, export rates and the formation of diatomârich sediment. Yellowstone Lake sediments should be an archive of past silicon biogeochemistry, although the effect of sublacustrine hydrothermal activity or hydrothermal explosion events is unclear. Here, we analysed lake water, tributaries, and hydrothermal vent fluids from Yellowstone Lake for their dissolved Si concentrations, isotope composition (30Si) and Ge/Si ratios to evaluate the sources of variability in the lakeâs Si cycle. Bulk elemental composition and biogenic SiO2 (bSiO2) content, together with 30Si and Ge/Si ratios from a single diatom species, Stephanodiscus yellowstonensis, were analysed in two sediment cores spanning the last 9880 cal. yr BP. We investigate these datasets to identify long term Holocene changes in hydrothermal activity and effects of large and short-term events i.e., hydrothermal and a volcanic eruption. Combinations of bSiO2, 30Si and Ge/Si with XRF and lithology data revealed that Yellowstone Lake has a resilient biogeochemical system: hydrothermal explosions are visible in the lithology but have no identifiable impact on bSiO2 accumulation or on the 30Si signature. Both cores show similarities that suggest a stable and homogeneous dSi source across the entire lake. A narrow range of 30Si and Ge/Si values suggests that the productive layer of the lake was well mixed and biogeochemically stable, with consistently high hydrothermal inputs of Si throughout the Holocene to buffer against the disturbance events. Variation in bSiO2 concentration through time is weakly correlated with an increase towards younger sediment in the 30Si fossil diatom record in both cores. This increase mirrors that seen in ocean records, and follows changes known in summer insolation, summer temperatures and lake water-column mixing since the deglaciation. This suggests that climate forcing, and soil formation ultimately govern the silicon isotope record, which we suggest is via a combination of changes in weathering stoichiometry, diatom production, and relative proportion of dSi sources
Quantifying nonâthermal silicate weathering using Ge/Si and Si isotopes in rivers draining the Yellowstone Plateau Volcanic Field, USA
In active volcanic regions, high-temperature chemical reactions in the hydrothermal system consume CO2 sourced from magma or from the deep crust, whereas reactions with silicates at shallow depths mainly consume atmospheric CO2. Numerous studies have quantified the load of dissolved solids in rivers that drain volcanic regions to determine chemical weathering rates and atmospheric CO2 consumption rates. However, the balance between thermal and non-thermal components to riverine fluxes in these areas remains poorly constrained, hindering accurate estimates of atmospheric CO2 consumption rates. Here we use the Ge/Si ratio and the stable silicon isotopes (ÎŽ30Si) as tracers for quantifying non-thermal silicon contributions in rivers draining the Yellowstone Plateau Volcanic Field, USA. The Ge/Si ratio (”mol.mol-1) was determined for 7 thermal water samples (183 ± 22), 8 rivers (35 ± 23) and 6 creeks flowing into Yellowstone Lake (5 ± 3) during base flow and during peak water discharge following snowmelt. The ÎŽ30Si value (â°) was determined for thermal waters (-0.09 ± 0.04), Yellowstone River at Yellowstone Lake outlet (1.91 ± 0.23) and creek samples (0.82 ± 0.29). The calculated atmospheric CO2 consumption associated with non-thermal waters flowing through Yellowstoneâs rivers during peak discharge is âŒ3.03 ton.km-2.yr-1, which is âŒ2 % of the annual mean atmospheric CO2 consumption in other volcanic regions. This study highlights the significance of quantifying seasonal variations in chemical weathering rates for improving estimates of atmospheric CO2 consumption rates in active volcanic regions