Study of solids conveying in single screw extruders based on flow dynamics and structure of solid pellets

Flow of granular matter is presently a subject of extensive research, due to the characteristics of this type of systems (e.g., dilatancy, segregation, arching, clustering) and relevance to various application areas, such as civil construction, agriculture, food processing, geophysics, pharmacology [1, 2]. The plasticating process in single screw polymer extrusion is one of the areas where this research can help to increase the existing knowledge. In the initial turns of an Archimedes-type screw, loose pellets are conveyed forward. However, traditional analyses assume the movement of an elastic solid plug at constant velocity. This work follows previous efforts to predict the characteristics of this flow using the Discrete Element Method (DEM) [3, 4]. Two boundary conditions are considered: a) open-discharge, implying that no compaction of the solids occurs and b) close-discharge, leading to a pressure increase. The dynamics and the structure of the flow were studied by computing the cross- and down channel velocity profiles, the coordination number distribution, the output rate, the residence time distribution and the density profile, as a function of the friction force grain-wall, screw speed and pellet size. The model is able to capture the process of plug formation towards the discharge, and its predictions provide an insight into possible flow fluctuations

Flow dynamics and structure of solid pellets along the channel of a single screw extruder

Plasticating single screw extrusion involves the progressive compaction and heating of loose solid pellets that eventually melt, form a relatively homogenous stream and are subsequently pumped through a shaping tool. Traditional analyses of the solids conveying stage assume the sliding of an elastic solid plug due to differential wall friction coefficients. However, not only the corresponding predictions may fail considerably, but it is also well known that, at least in the initial screw turns, pellets are far from compact. This work follows previous efforts to model the flow of solids in the hopper and initial screw turns using the Discrete Element Method (DEM). The model considers the development of normal and tangential forces resulting from the inelastic collisions between the pellets and between them and the neighbouring metallic surfaces. As an example of the capability of the model to capture detailed features of granular flow, the effect of pellet size on flow is discussed.Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BPD/39381/2007

Incorporating tool deformation in the design of extrusion dies for complex hollow profiles

The potential of OpenFOAM to design extrusion dies, incorporating the Fluid Structure Interaction (FSI)Fundação para a Ciência e a Tecnologia (FCT) - FCOMP-01-0124-FEDER-015126 (Refª. FCT PTDC/EMEMFE/ 113988/2009), FCOMP-01-0124-FEDER-010190 (Refª. FCT PTDC/EME-FME/102729/2008) and PEst-C/CTM/LA0025/2011 (Strategic Project - LA 25 – 2011-2012), SFRH/BD43632/2008, FCT-SFRH/ BPD/ 77467/ 201

Study of the piezoresistivity of doped nanocrystalline silicon thin films

The piezoresistive response of n- and p-type hydrogenated nanocrystalline silicon thin films, deposited by hot-wire (HW) and plasma-enhanced chemical vapor deposition (PECVD) on thermally oxidized silicon wafers, has been studied using four-point bending tests. The piezoresistive gauge factor (GF) was measured on patterned thin-film micro-resistors rotated by an angle θ with respect to the principal strain axis. Both longitudinal (GFL) and transverse (GFT) GFs, corresponding to θ = 0° and 90°, respectively, are negative for n-type and positive for p-type films. For other values of θ (30°, 45°, 120°, and 135°) GFs have the same signal as GFL and GFT and their value is proportional to the normal strain associated with planes rotated by θ relative to the principal strain axis. It is concluded that the films are isotropic in the growth plane since the GF values follow a Mohr’s circle with the principal axes coinciding with those of the strain tensor. The strongest p-type pirezoresistive response (GFL = 41.0, GFT = 2.84) was found in a film deposited by PECVD at a substrate temperature of 250 °C and working pressure of 0.250 Torr, with dark conductivity 1.6 Ω−1cm−1. The strongest n-type response (GFL =− 28.1, GFT =− 5.60) was found in a film deposited by PECVD at 150 °C and working pressure of 3 Torr, with dark conductivity 9.7 Ω−1cm−1. A model for the piezoresistivity of nc-Si is proposed, based on a mean-field approximation for the conductivity of an ensemble of randomly oriented crystallites and neglecting grain boundary effects. The model is able to reproduce the measured GFL values for both n- and p-type films. It fails, however, to explain the transversal GFT data. Both experimental and theoretical data show that nanocrystalline silicon can have an isotropic piezoresistive effect of the order of 40% of the maximum response of crystalline silicon.Fundação para a Ciência e a Tecnologia (FCT) - PTDC/CTM/66558/2006, bolsa de investigação SFRH/BSAB/883/200

Bianchi VIII Empty Futures

Using a qualitative analysis based on the Hamiltonian formalism and the orthonormal frame representation we investigate whether the chaotic behaviour which occurs close to the initial singularity is still present in the far future of Bianchi VIII models. We describe some features of the vacuum Bianchi VIII models at late times which might be relevant for studying the nature of the future asymptote of the general vacuum inhomogeneous solution to the Einstein field equations.Comment: 22 pages, no figures, Latex fil

Changing concepts in plant hormone action

Summary: A plant hormone is not, in the classic animal sense, a chemical synthesized in one organ, transported to a second organ to exert a chemical action to control a physiological event. Any phytohormone can be synthesized everywhere and can influence different growth and development processes at different places. The concept of physiological activity under hormonal control cannot be dissociated from changes in concentrations at the site of action, from spatial differences and changes in the tissue's sensitivity to the compound, from its transport and its metabolism, from balances and interactions with the other phytohormones, or in their metabolic relationships, and in their signaling pathways as well. Secondary messengers are also involved. Hormonal involvement in physiological processes can appear through several distinct manifestations (as environmental sensors, homeostatic regulators and spatio-temporal synchronizers, resource allocators, biotime adjusters, etc.), dependent on or integrated with the primary biochemical pathways. The time has also passed for the hypothesized ‘specific' developmental hormones, rhizocaline, canlocaline, and florigen: root, stem, and flower formation result from a sequential control of specific events at the right places through a coordinated control by electrical signals, the known phytohormones and nonspecific molecules of primary and secondary metabolism, and involve both cytoplasmic and apoplastic compartments. These contemporary views are examined in this revie

Modelling pellet flow in single extrusion with DEM

Plasticating single-screw extrusion involves the continuous conversion of loose solid pellets into a pressurized homogeneous melt that is pumped through a shaping tool. Traditional analyses of the solids conveying stage assume the movement of an elastic solid plug at a fixed speed. However, not only the corresponding predictions fail considerably, but it is also well known that, at least in the initial screw turns, the flow of loose individual pellets takes place. This study follows previous efforts to predict the characteristics of such a flow using the discrete element method. The model considers the development of normal and tangential forces resulting from the inelastic collisions between the pellets and between them and the neighbouring metallic surfaces. The algorithm proposed here is shown to be capable of capturing detailed features of the granular flow. The predictions of velocities in the cross- and down-channel directions and of the coordination number are in good agreement with equivalent reported results. The effect of pellet size on the flow features is also discussed

Control and Monitoring of the Online Computer Farm for Offline Processing in LHCb

ISBN 978-3-95450-139-7 - http://accelconf.web.cern.ch/AccelConf/ICALEPCS2013/papers/tuppc063.pdfInternational audienceLHCb, one of the 4 experiments at the LHC accelerator at CERN, uses approximately 1500 PCs (averaging 12 cores each) for processing the High Level Trigger (HLT) during physics data taking. During periods when data acquisition is not required most of these PCs are idle. In these periods it is possible to profit from the unused processing capacity to run offline jobs, such as Monte Carlo simulation. The LHCb offline computing environment is based on LHCbDIRAC (Distributed Infrastructure with Remote Agent Control). In LHCbDIRAC, job agents are started on Worker Nodes, pull waiting tasks from the central WMS (Workload Management System) and process them on the available resources. A Control System was developed which is able to launch, control and monitor the job agents for the offline data processing on the HLT Farm. This control system is based on the existing Online System Control infrastructure, the PVSS SCADA and the FSM toolkit. It has been extensively used launching and monitoring 22.000+ agents simultaneously and more than 850.000 jobs have already been processed in the HLT Farm. This paper describes the deployment and experience with the Control System in the LHCb experiment

In Vitro CRISPR/Cas9 Transfection and Gene-Editing Mediated by Multivalent Cationic LiposomeDNA Complexes

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated nuclease 9 (Cas9) gene-editing offers exciting new therapeutic possibilities for disease treatment with a genetic etiology such as cancer, cardiovascular, neuronal, and immune disorders. However, its clinical translation is being hampered by the lack of safe, versatile, and effective nonviral delivery systems. Herein we report on the preparation and application of two cationic liposome–DNA systems (i.e., lipoplexes) for CRISPR/Cas9 gene delivery. For that purpose, two types of cationic lipids are used (DOTAP, monovalent, and MVL5, multivalent with +5e nominal charge), along with three types of helper lipids (DOPC, DOPE, and monoolein (GMO)). We demonstrated that plasmids encoding Cas9 and single-guide RNA (sgRNA), which are typically hard to transfect due to their large size (>9 kb), can be successfully transfected into HEK 293T cells via MVL5-based lipoplexes. In contrast, DOTAP-based lipoplexes resulted in very low transfection rates. MVL5-based lipoplexes presented the ability to escape from lysosomes, which may explain the superior transfection efficiency. Regarding gene editing, MVL5-based lipoplexes achieved promising GFP knockout levels, reaching rates of knockout superior to 35% for charge ratios (+/−) of 10. Despite the knockout efficiency being comparable to that of Lipofectamine 3000® commercial reagent, the non-specific gene knockout is more pronounced in MVL5-based formulations, probably resulting from the considerable cytotoxicity of these formulations. Altogether, these results show that multivalent lipid-based lipoplexes are promising CRISPR/Cas9 plasmid delivery vehicles, which by further optimization and functionalization may become suitable in vivo delivery systems.This research was funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte and the Project FCOMP-01– 0124-FEDER-021053 (PTDC/SAU-BMA/121028/2010). This research was also supported by the Microfluidic Layer-by-layer Assembly of Cationic Liposome—Nucleic Acid Nanoparticles for Gene Delivery project (032520) co-funded by FCT and the ERDF through COMPETE2020. Diana A. Sousa (D.A.S) and Celso J.O. Ferreira (C.J.O.F) acknowledge FCT for the grants PD/BD/139083/2018 and SFRH/BD/149199/2019, respectively.info:eu-repo/semantics/publishedVersio

Mismatch repair SNPs and thyroid cancer susceptibility: a potential role for the MSH6 rs1042821 (Gly39Glu) polymorphism

Abstract of the Poster presented at the 3rd ESPT Conference (European Society of Pharmacogenomics and Personalised Therapy / European Society of Pharmacogenomics and Theranostics), 7-9 October 2015, Budapest