From parasite genomes to one healthy world: Are we having fun yet?

In 1990, the Human Genome Sequencing Project was established. This laid the ground work for an explosion of sequence data that has since followed. As a result of this effort, the first complete genome of an animal, Caenorhabditis elegans was published in 1998. The sequence of Drosophila melanogaster was made available in March, 2000 and in the following year, working drafts of the human genome were generated with the completed sequence (92%) being released in 2003. Recent advancements and next-generation technologies have made sequencing common place and have infiltrated every aspect of biological research, including parasitology. To date, sequencing of 32 apicomplexa and 24 nematode genomes are either in progress or near completion, and over 600k nematode EST and 200k apicomplexa EST submissions fill the databases. However, the winds have shifted and efforts are now refocusing on how best to store, mine and apply these data to problem solving. Herein we tend not to summarize existing X-omics datasets or present new technological advances that promise future benefits. Rather, the information to follow condenses up-to-date-applications of existing technologies to problem solving as it relates to parasite research. Advancements in non-parasite systems are also presented with the proviso that applications to parasite research are in the making

A multiplex PCR assay for differentiating economically important gastrointestinal nematodes of cattle

A multiplex polymerase chain reaction (PCR) test was developed for identifying gastrointestinal (GI) nematodes that commonly infect cattle. This assay was developed using adult-derived genomic DNA and shown capable of discriminating parasite eggs from the feces of experimentally-infected animals at both the species and genus levels. Sequence data from internal (ITS) and external (ETS) transcribed spacers of the ribosomal DNA (rDNA) repeats as well as the 3′-end of the small subunit rDNA and 5′-end of the large subunit rDNA were used to generate five primer sets which, when used simultaneously in a multiplex PCR, produce a unique electrophoretic DNA banding pattern charac- terized by a single DNA fragment for Ostertagia ostertagi (257 bp), Haemonchus placei (176 bp),Oesophagostomum radiatum (329 bp), Trichostrongylus colubriformis (243 bp) and Cooperia on- cophora (151 bp). In a similar manner, the constructed primer sets amplified DNA from Ostertagia lyrata, Haemonchus contortus, Trichostrongylus axei, Cooperia surnabada and Cooperia punctata. With respect to H. contortus, a closely migrating doublet was generated suggesting size heterogene- ity in the ETS which is consistent with multiple rDNA repeat units within this species. PCR analyses using mixtures of monospecifically-purified nematode eggs indicated a sensitivity of less than 0.5 egg-DNA equivalent per species. Although, not designed as a quantitative technique, relative PCR signal intensities corresponded to relative egg burdens within the DNA samples from mixed species of eggs. Published by Elsevier Science B.V

New application of powder injection molded product in medical field

Nowadays, majority part of powder injection molding (PIM) market in Europe consists in automotive (43 %). In contrast, to medical applications only 13 % of market is devoted. This paper is focused on a new design and production technology of the adenoid cutting curette used in otorhinolaryngology. In the theoretical part, the present design issues of the cutting curette are shown, and time consumption and wear problems of sterilisation are described. Experimental part consists in selection of suitable metal powder for medical application, computeraided engineering (CAE) Moldflow analysis of proper gating system followed by construction of injection mold and production of real samples. The new design of replaceable cutting edge is easily customized according to various shapes of patient oral cavity and for doctor's need. © 2016. Published by Manufacturing Technology

Thermal Model Correlation for Mars Reconnaissance Orbiter

The Mars Reconnaissance Orbiter (MRO) launched on August 12, 2005 and began aerobraking at Mars in March 2006. In order to save propellant, MRO used aerobraking to modify the initial orbit at Mars. The spacecraft passed through the atmosphere briefly on each orbit; during each pass the spacecraft was slowed by atmospheric drag, thus lowering the orbit apoapsis. The largest area on the spacecraft, most affected by aeroheating, was the solar arrays. A thermal analysis of the solar arrays was conducted at NASA Langley Research Center to simulate their performance throughout the entire roughly 6-month period of aerobraking. A companion paper describes the development of this thermal model. This model has been correlated against many sets of flight data. Several maneuvers were performed during the cruise to Mars, such as thruster calibrations, which involve large abrupt changes in the spacecraft orientation relative to the sun. The data obtained from these maneuvers allowed the model to be well-correlated with regard to thermal mass, conductive connections, and solar response well before arrival at the planet. Correlation against flight data for both in-cruise maneuvers and drag passes was performed. Adjustments made to the model included orientation during the drag pass, solar flux, Martian surface temperature, through-array resistance, aeroheating gradient due to angle of attack, and aeroheating accommodation coefficient. Methods of correlation included comparing the model to flight temperatures, slopes, temperature deltas between sensors, and solar and planet direction vectors. Correlation and model accuracy over 400 aeroheating drag passes were determined, with overall model accuracy better than 5 C

Melhoramento genético: a nova arma no controle de doenças.

bitstream/item/117255/1/cnpc-2000-Melhoramento.pd

Thermal Modeling of the Mars Reconnaissance Orbiter's Solar Panel and Instruments during Aerobraking

The Mars Reconnaissance Orbiter (MRO) launched on August 12, 2005 and started aerobraking at Mars in March 2006. During the spacecraft s design phase, thermal models of the solar panels and instruments were developed to determine which components would be the most limiting thermally during aerobraking. Having determined the most limiting components, thermal limits in terms of heat rate were established. Advanced thermal modeling techniques were developed utilizing Thermal Desktop and Patran Thermal. Heat transfer coefficients were calculated using a Direct Simulation Monte Carlo technique. Analysis established that the solar panels were the most limiting components during the aerobraking phase of the mission

A Comparison of Platforms for the Aerial Exploration of Titan

Exploration of Titan, envisioned as a follow-on to the highly successful Cassini-Huygens mission, is described in this paper. A mission blending measurements from a dedicated orbiter and an in-situ aerial explorer is discussed. Summary description of the science rationale and the mission architecture, including the orbiter, is provided. The mission has been sized to ensure it can be accommodated on an existing expendable heavy-lift launch vehicle. A launch to Titan in 2018 with a 6-year time of flight to Titan using a combination of Solar Electric Propulsion and aeroassist (direct entry and aerocapture) forms the basic mission architecture. A detailed assessment of different platforms for aerial exploration of Titan has been performed. A rationale for the selection of the airship as the baseline platform is provided. Detailed description of the airship, its subsystems, and its operational strategies are provided

The CALIPSO Integrated Thermal Control Subsystem

The Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation (CALIPSO) is a joint NASA-CNES mission to study the Earth's cloud and aerosol layers. The satellite is composed of a primary payload (built by Ball Aerospace) and a spacecraft platform bus (PROTEUS, built by Alcatel Alenia Space). The thermal control subsystem (TCS) for the CALIPSO satellite is a passive design utilizing radiators, multi-layer insulation (MLI) blankets, and both operational and survival surface heaters. The most temperature sensitive component within the satellite is the laser system. During thermal vacuum testing of the integrated satellite, the laser system's operational heaters were found to be inadequate in maintaining the lasers required set point. In response, a solution utilizing the laser system's survival heaters to augment the operational heaters was developed with collaboration between NASA, CNES, Ball Aerospace, and Alcatel-Alenia. The CALIPSO satellite launched from Vandenberg Air Force Base in California on April 26th, 2006. Evaluation of both the platform and payload thermal control systems show they are performing as expected and maintaining the critical elements of the satellite within acceptable limits

Thermal Analysis Methods for Aerobraking Heating

As NASA begins exploration of other planets, a method of non-propulsively slowing vehicles at the planet, aerobraking, may become a valuable technique for managing vehicle design mass and propellant. An example of this is Mars Reconnaissance Orbiter (MRO), which will launch in late 2005 and reach Mars in March of 2006. In order to save propellant, MRO will use aerobraking to modify the initial orbit at Mars. The spacecraft will dip into the atmosphere briefly on each orbit, and during the drag pass, the atmospheric drag on the spacecraft will slow it, thus lowering the orbit apoapsis. The largest area on the spacecraft, and that most affected by the heat generated during the aerobraking process, is the solar arrays. A thermal analysis of the solar arrays was conducted at NASA Langley, to simulate their performance throughout the entire roughly 6-month period of aerobraking. Several interesting methods were used to make this analysis more rapid and robust. Two separate models were built for this analysis, one in Thermal Desktop for radiation and orbital heating analysis, and one in MSC.Patran for thermal analysis. The results from the radiation model were mapped in an automated fashion to the Patran thermal model that was used to analyze the thermal behavior during the drag pass. A high degree of automation in file manipulation as well as other methods for reducing run time were employed, since toward the end of the aerobraking period the orbit period is short, and in order to support flight operations the runs must be computed rapidly. All heating within the Patran Thermal model was combined in one section of logic, such that data mapped from the radiation model and aeroheating model, as well as skin temperature effects on the aeroheating and surface radiation, could be incorporated easily. This approach calculates the aeroheating at any given node, based on its position and temperature as well as the density and velocity at that trajectory point. Run times on several different processors, computer hard drives, and operating systems (Windows versus Linux) were evaluated

A multiplex PCR assay for differentiating economically important gastrointestinal nematodes of cattle

A multiplex polymerase chain reaction (PCR) test was developed for identifying gastrointestinal (GI) nematodes that commonly infect cattle. This assay was developed using adult-derived genomic DNA and shown capable of discriminating parasite eggs from the feces of experimentally-infected animals at both the species and genus levels. Sequence data from internal (ITS) and external (ETS) transcribed spacers of the ribosomal DNA (rDNA) repeats as well as the 3′-end of the small subunit rDNA and 5′-end of the large subunit rDNA were used to generate five primer sets which, when used simultaneously in a multiplex PCR, produce a unique electrophoretic DNA banding pattern charac- terized by a single DNA fragment for Ostertagia ostertagi (257 bp), Haemonchus placei (176 bp),Oesophagostomum radiatum (329 bp), Trichostrongylus colubriformis (243 bp) and Cooperia on- cophora (151 bp). In a similar manner, the constructed primer sets amplified DNA from Ostertagia lyrata, Haemonchus contortus, Trichostrongylus axei, Cooperia surnabada and Cooperia punctata. With respect to H. contortus, a closely migrating doublet was generated suggesting size heterogene- ity in the ETS which is consistent with multiple rDNA repeat units within this species. PCR analyses using mixtures of monospecifically-purified nematode eggs indicated a sensitivity of less than 0.5 egg-DNA equivalent per species. Although, not designed as a quantitative technique, relative PCR signal intensities corresponded to relative egg burdens within the DNA samples from mixed species of eggs. Published by Elsevier Science B.V