546 research outputs found
Temporal tracking of mineralization and transcriptional developments of shell formation during the early life history of pearl oyster Pinctada maxima
Molluscan larval ontogeny is a highly conserved process comprising three principal developmental stages. A characteristic unique to each of these stages is shell design, termed prodissoconch I, prodissoconch II and dissoconch. These shells vary in morphology, mineralogy and microstructure. The discrete temporal transitions in shell biomineralization between these larval stages are utilized in this study to investigate transcriptional involvement in several distinct biomineralization events. Scanning electron microscopy and X-ray diffraction analysis of P. maxima larvae and juveniles collected throughout post-embryonic ontogenesis, document the mineralogy and microstructure of each shelled stage as well as establishing a timeline for transitions in biomineralization. P. maxima larval samples most representative of these biomineralization distinctions and transitions were analyzed for differential gene expression on the microarray platform PmaxArray 1.0. A number of transcripts are reported as differentially expressed in correlation to the mineralization events of P. maxima larval ontogeny. Some of those isolated are known shell matrix genes while others are novel; these are discussed in relation to potential shell formation roles. This interdisciplinary investigation has linked the shell developments of P. maxima larval ontogeny with corresponding gene expression profiles, furthering the elucidation of shell biomineralization
A review of trauma and orthopaedic randomised clinical trials published in high-impact general medical journals
Acknowledgements The authors are grateful for Shaun Treweek for his expert insight into the nuances of Randomised Clinical Trial design. Funding The authors did not receive support from any organisation for the submitted work.Peer reviewedPublisher PD
The influence of obesity on falls and quality of life
This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens
H-Diplo Roundtable XXII-30 on Nichter. The Last Brahmin: Henry Cabot Lodge Jr. and the Making of the Cold War
A set of reviews of Luke A. Nichter\u27s The Last Brahmin: Henry Cabot Lodge Jr. and the Making of the Cold War, with a response from the author
- ā¦