56 research outputs found
Evolutionary and Experimental Assessment of Novel Markers for Detection of Xanthomonas euvesicatoria in Plant Samples
BACKGROUND: Bacterial spot-causing xanthomonads (BSX) are quarantine phytopathogenic bacteria responsible for heavy losses in tomato and pepper production. Despite the research on improved plant spraying methods and resistant cultivars, the use of healthy plant material is still considered as the most effective bacterial spot control measure. Therefore, rapid and efficient detection methods are crucial for an early detection of these phytopathogens. METHODOLOGY: In this work, we selected and validated novel DNA markers for reliable detection of the BSX Xanthomonas euvesicatoria (Xeu). Xeu-specific DNA regions were selected using two online applications, CUPID and Insignia. Furthermore, to facilitate the selection of putative DNA markers, a customized C program was designed to retrieve the regions outputted by both databases. The in silico validation was further extended in order to provide an insight on the origin of these Xeu-specific regions by assessing chromosomal location, GC content, codon usage and synteny analyses. Primer-pairs were designed for amplification of those regions and the PCR validation assays showed that most primers allowed for positive amplification with different Xeu strains. The obtained amplicons were labeled and used as probes in dot blot assays, which allowed testing the probes against a collection of 12 non-BSX Xanthomonas and 23 other phytopathogenic bacteria. These assays confirmed the specificity of the selected DNA markers. Finally, we designed and tested a duplex PCR assay and an inverted dot blot platform for culture-independent detection of Xeu in infected plants. SIGNIFICANCE: This study details a selection strategy able to provide a large number of Xeu-specific DNA markers. As demonstrated, the selected markers can detect Xeu in infected plants both by PCR and by hybridization-based assays coupled with automatic data analysis. Furthermore, this work is a contribution to implement more efficient DNA-based methods of bacterial diagnostics
Relevance of Dynamic Strain Aging under Quasi-Static Tension on AISI 304 Stainless Steel
High Temperature Plastic Instability and Dynamic Strain Aging in the Tensile Behavior of AISI 316 Stainless Steel
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The orbit and stellar masses of the archetype colliding-wind binary WR 140
10 pages, 5 figuresThis is the final version. Available from Oxford University Press via the DOI in this recordData availability: All measurements used in this analysis are tabulated either in this paper or included in cited references.We present updated orbital elements for the Wolf-Rayet (WR) binary WR 140 (HD 193793; WC7pd + O5.5fc). The new orbital elements were derived using previously published measurements along with 160 new radial velocity measurements across the 2016 periastron passage of WR 140. Additionally, four new measurements of the orbital astrometry were collected with the CHARA Array. With these measurements, we derive stellar masses of and . We also include a discussion of the evolutionary history of this system from the Binary Population and Spectral Synthesis model grid to show that this WR star likely formed primarily through mass-loss in the stellar winds, with only a moderate amount of mass lost or transferred through binary interactions.University of ToledoNASAEuropean Union Horizon 202
Contrasting effects of ultraviolet radiation on the growth efficiency of freshwater bacteria
KVĖPAVIMO PRO BURNĄ IR KITŲ NETAISYKLINGŲ ĮPROČIŲ PAPLITIMAS TARP IKIMOKYKLINIO AMŽIAUS VAIKŲ
Prevalence of bruxism and temporomandibular disorders among orphans in southeast Uganda: A gender and age comparison
Oral Habits: Prevalence and Effects on Occlusion Among 7 to 13 Years Old School Children in Aseer, Saudi Arabia
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