A randomised trial of the bone formation after maxillary sinus floor augmentation with bovine hydroxyapatite (Cerabone®) and Photobiomodulation: histomorphometric and immunohistochemical analysis

The use of non-autogenous biomaterial to increase bone height in the maxillary sinus has been shown to be effective, but the results are still inconclusive.Eight participants were selected and included in the research. After surgical access with osteotomy on the lateral wall of both maxillary sinuses, these were filled with Cerabone®. Then, by blind randomization, they received one of the following treatments: Filling with Cerabone® (Control group); treatment with Photobiomodulation (PBM), filling with Cerabone® and treatment with low-power laser (PBM group). Biopsies were obtained 30 days after the surgery, using a 2.8 mm internal diameter trephine bur. Qualitative and quantitative histological analyzes were performed and immunohistochemical analyzes of osteocalcin (OCN) and tartrate-resistant acid phosphatase (TRAP) were performed with scores for each of the biological events.The Cerabone® biomaterial demonstrated a high degree of biocompatibility. New bone formation was observed in both groups. In the PBM group, there was greater bone formation and newly formed tissue in an advanced state of bone maturation. The immunostaining of OCN was greater at 30 days in the PBM group than in the control. There was no significant difference in TRAP immunostaining at 30 days between the groups.Low-power laser-mediated by PBM promoted greater bone formation; the newly formed tissue showed a more advanced state of bone maturation in maxillary sinuses filled with Cerabone® biomaterial and treatment with PBM, within the 30-day evaluation period

In vivo comparative study of the effects of using the enamel matrix derivative and/or photobiomodulation on the repair of bone defects

The repair of bone defects has been the subject of many studies that have shown inconclusive results as to what is the best bone substitute. Bone defects (Ø 2 mm) were induced on the tibia of seventy-two rats, which were distributed into the following

Experimental periodontal disease treatment by subgingival irrigation with tetracycline hydrochloride in rats

OBJECTIVE: The aim of this study was to compare subgingival irrigation with tetracycline hydrochloride (TTC-HCL) as adjunctive treatment to scaling and root planning (SRP) on induced periodontitis in rats. MATERIAL AND METHODS: In 60 rats, periodontal disease was ligature-induced at the mandibular left first molar. After 7 days, the ligature was removed and all animals were submitted to SRP, and divided into 2 groups according to the following treatment: C (n=30) - subgingival irrigation with 1 mL of saline; T (n=30) - subgingival irrigation with 1 mL of TTC-HCL (50 mg/mL). Ten animals in each group were euthanized at 7, 15 and 30 days posttreatment. The histometric values were statistically analyzed (p<0.05). RESULTS: In the histometric analysis, at 7, 15 and 30 days, Group T (0.72±0.05 mm(2), 0.57±0.14 mm(2), 0.62±0.07 mm(2)), showed less bone loss (p<0.05) than Group C (1.35±0.25 mm(2); 1.40±0.31 mm(2); 1.29±0.27 mm(2)), respectively. CONCLUSIONS: Subgingival irrigation with TTC-HCL was an effective adjunctive treatment for periodontal disease induced in rats

Effects of a single near-infrared laser treatment on cutaneous wound healing: Biometrical and histological study in rats

Background: Low intensity laser therapy has been recommended to support the cutaneous repair; however, so far studies do not have evaluated the tissue response following a single laser treatment. This study investigated the effect of a single laser irradiation on the healing of full-thickness skin lesions in rats.Methods: Forty-eight male rats were randomly divided into three groups. One surgical lesion was created on the back of rats using a punch of 8 mm in diameter. One group was not submitted to any treatment after surgery and it was used as control. Two energy doses from an 830-nm near-infrared diode laser were used immediately post-wounding: 1.3 J cm(-2) and 3 J cm(-2). The laser intensity 53 mW cm(-2) was kept for both groups. Biometrical and histological analyses were accomplished at days 3, 7 and 14 post-wounding.Results: Irradiated lesions presented a more advanced healing process than control group. The dose of 1.3 J cm(-2) leaded to better results. Lesions of the group irradiated with 1.3 J cm(-2) presented faster lesion contraction showing quicker re-epithelization and reformed connective tissue with more organized collagen fibers.Conclusions: Low-intensity laser therapy may accelerate cutaneous wound healing in a rat model even if a single laser treatment is performed. This finding might broaden current treatment regimens. (c) 2007 Elsevier B.V. All rights reserved

Periodontal Tissue Response to Coverage of Root Cavities Restored With Resin Materials: A Histomorphometric Study in Dogs

Background: The purpose of this study was to histomorphometrically evaluate the response of periodontal tissues covering Class V resin restorations in dogs. Methods: After raising a mucoperiosteal flap, bony defects measuring 5 · 5 mm were created on the buccal aspect of the canines of five dogs followed by cavity preparations on the root surface measuring 3 · 3 · 1 mm. Before repositioning the flap to cover the bone defect, the cavities were restored with composite resin (CR) or resin-modified glass ionomer cement (RMGIC) or were left unrestored as control (C). The dogs were euthanized 90 days after surgery. Specimens comprising the tooth and periodontal tissues were removed, processed routinely, cut into longitudinal serial sections in the buccolingual direction, and stained with hematoxylin and eosin (H&amp;E) or Masson&apos;s trichrome. The most central sections were selected for histomorphometric analysis. Results: Histomorphometric analysis revealed apical migration of epithelial tissue onto the restorative materials (RMGIC and CR). The C group presented significantly longer connective tissue attachment (P &lt;0.05) than the RMGIC and CR groups and significantly higher bone regeneration (P &lt;0.05) compared to the RMGIC group. Histologically, the cervical third (CT) of all groups had the most marked chronic inflammatory infiltrate. Conclusions: Within the limits of this study, it can be concluded that the restorative materials used exhibit biocompatibility; however, both materials interfered with the development of new bone and the connective tissue attachment process

Antibiotics plus probiotic as an adjunct to the treatment of periodontitis in smokers: a short-term study

Aim: The purpose of this study was to perform a short-term evaluation of the effects of a probiotic, associated with the use of antibiotics as an adjuvant treatment of periodontitis in smokers. Materials and Methods: Thirty-four patients were randomly divided into: PRO group (n = 17), patients received a single session of scaling and root planing (SRP) associated with the systemic administration of Lactobacillus reuteri tablets; ANT+PRO group (n=17), patients received a single session of SRP followed by the systemic administration of Amoxicillin and Metronidazole 3x/day for 7 days and administration of Lactobacillus reuteri seven days after conclusion of antibiotic therapy. Clinical periodontal parameters were evaluated. Results: After treatment, both groups showed a reduction in BOP, PI, and residual pockets compared to baseline, while in the ANT+PRO group there was a significant reduction in PD and CAL gain (p5mm was observed in the ANT+PRO group (p<0.05). Conclusion: Both adjuvant treatments reduced the residual pockets and controlled inflammation after treatment. Only the association of antibiotic with probiotic therapy was able to efficiently reduce mean PD, the number of pockets with PD >5 mm, and promote additional CAL gains in a short-term evaluation period

Effectiveness of two methods for preparation of autologous platelet-rich plasma: an experimental study in rabbits

The purpose of this study was to compare the quantity and quality of platelets in platelet-rich plasma (PRP) samples prepared using either the single- or the double-centrifugation protocol. Ten adult white New Zealand rabbits were used. Ten ml of blood were drawn from each animal via cardiac puncture. Each blood sample was divided into two equal parts for PRP preparation: 5 ml of blood were centrifuged according to a single-centrifugation protocol (Group I), and 5 ml were centrifuged according to a double-centrifugation protocol (Group II). Manual platelet counts were performed on the whole blood and PRP samples of each group. Smears were also done on all samples in order to see the morphology of the platelets. The data obtained in the manual platelet count were submitted to statistical analysis (repeated measures ANOVA, Tukey, P<.05). The average whole blood platelet count was 446,389/μl. The PRP samples in Group II presented an average platelet amount significantly higher than that of Group I (1,986,875 ± 685,020/μl and 781,875 ± 217,693/μl, respectively). The PRP smears from Group II were the only one to present platelets with altered morphology (75% of the smears). A few lymphocytes with increased cytoplasm were observed in the PRP smears of both Groups I (25% of the smears) and II (62.5% of the smears). Within the limits of this study, it can be concluded that the double-centrifugation protocol resulted in higher platelet concentrations than did the single-centrifugation protocol. However, the double-centrifugation protocol caused alterations in platelet morphology and was more sensitive to small processing errors

Effect of platelet-rich plasma on bone healing of autogenous bone grafts in critical-size defects

AimThis study histologically analysed the effect of autogenous platelet-rich plasma (PRP), prepared according to a new semiautomatic system, on healing of autogenous bone (AB) grafts placed in surgically created critical-size defects (CSD) in rabbit calvaria.Material and MethodsSixty rabbits were divided into three groups: C, AB and AB/PRP. A CSD was created in the calvarium of each animal. In Group C (control), the defect was filled by blood clot only. In Group AB (autogenous bone graft), the defect was filled with particulate autogenous bone. In Group AB/PRP (autogenous bone graft with platelet-rich plasma), it was filled with particulate autogenous bone combined with PRP. All groups were divided into subgroups (n=10) and euthanized at 4 or 12 weeks post-operatively. Histometric and histologic analyses were performed. Data were statistically analysed (anova, t-test, p 0.05). It is notable that the amount of new bone formation in Group AB/PRP at 4 weeks was similar to that of Group AB at 12 weeks (p > 0.05).ConclusionWithin its limitation, the present study has indicated that (i) AB and AB/PRP significantly improved bone formation and (ii) a beneficial effect of PRP was limited to an initial healing period of 4 weeks