The efficacy of paravertebral blockades in the complex treatment of patients with the combination of neurological symptoms of cervical-thoracic osteochondrosis and ischemic disease

Sixty-seven patients with clinical symptoms of cervical-thoracic osteochondrosis and ischemic disease have been studied. In the main group included 43 patients, paravertebral blockades (PVB) were applied and in the control group (24 patients) - antianginal and metabolic therapy was used. Treatment efficacy was assessed by the dynamics of pain syndrome parameters as measured with the visual analogue scale and by changes of heart rhythm variability, ischemic disturbances and arrhythmia measured by Holter monitoring. The higher therapeutic efficacy of PVB in the complex treatment of these patients comparing to traditional treatment of ischemic disease was found

The efficacy of paravertebral blockades in the complex treatment of patients with the combination of neurological symptoms of cervical-thoracic osteochondrosis and ischemic disease

Sixty-seven patients with clinical symptoms of cervical-thoracic osteochondrosis and ischemic disease have been studied. In the main group included 43 patients, paravertebral blockades (PVB) were applied and in the control group (24 patients) - antianginal and metabolic therapy was used. Treatment efficacy was assessed by the dynamics of pain syndrome parameters as measured with the visual analogue scale and by changes of heart rhythm variability, ischemic disturbances and arrhythmia measured by Holter monitoring. The higher therapeutic efficacy of PVB in the complex treatment of these patients comparing to traditional treatment of ischemic disease was found

Detection of the PRAME Protein on the Surface of Melanoma Cells Using a Fluorescently Labeled Monoclonal Antibody

Abstract: Determination of the expression level of tumor marker PRAME protein is important both for predicting the course of the disease and for monitoring the effectiveness of anticancer therapy. A fluorescently labeled monoclonal antibody to the PRAME protein was obtained by periodate oxidation of glycans followed by modification with a bifunctional azido-oxyamine reagent and a “click” reaction with alkyne-modified sulfonated cyanine dye Cy3. A new approach to the synthesis of a bifunctional azido-oxyamine reagent using the ethoxyethylidene protecting group for oxyamine is proposed. The labeled antibodies were characterized with UV/Vis absorption spectra and the stoichiometry of the modification was determined. It has been demonstrated that the fluorescent antibodies retain affinity and can be used as a diagnostic tool for detecting the residual marker (the PRAME protein) after anticancer therapy. © 2021, Pleiades Publishing, Ltd

Supplementary Material for: The Trajectory of Successful Aging: Insights from Metagenome and Cytokine Profiling

Introduction: The longevity is influenced by of genetic, environmental, and lifestyle factors. The specific changes that occur in the gut microbiome during the aging process, and their relationship to longevity and immune function, have not yet been fully understood. The ongoing research other microbiome based to longevity cohort in Kazakhstan, provides preliminary information on longevity-related aging, where cytokine expression is associated with specific microbial communities and microbial functions. Methods: Metagenomic shotgun sequencing study of 40 long-lived individuals aged 90 years and over were carried out, who were conditionally healthy and active, able to served themselves, without a history of serious infection and cancer, who had not taken any antimicrobials, including probiotics. Blood serum weas analyzed to clinical and laboratory characteristics. The cytokine and chemokine profile in serum and stool samples was assessed using Multiplex analysis. Results: We found a significant increase in the expression of pro-inflammatory cytokines IL-1a, IL-6, 12p70, IP-10, IFN-α2, IL-15 TNFa, as well as chemokines MIP-1a/CCL3 and MIP-1b/CCL4, chemokine motif ligands MCP-3/CCL7 and MDC/CCL22(1c). Nonagenerians and centenarians demonstrated a greater diversity of core microbiota genera and showed an elevated prevalence of the genera Bacteroides, Clostridium, Escherichia and Alistipes. Conversely, there was a decrease in the abundance of the genera Ruminococcaceae, Fusicatenibacter, Dorea, as well as the species Fusicatenibacter saccharivorans. Furthermore, functional analysis revealed that the microbiome in long-lived group has a high capacity for lipid metabolism, amino acid degradation, and potential signs of chronic inflammatory status. Conclusion: Long-lived individuals exhibit an immune system imbalance and observed changes in the composition of the gut microbiota at the genus level between to the two age groups. Age-related changes in the gut microbiome, metabolic functions of the microbial community, and chronic inflammation all contribute to immunosenescence. In turn, the inflammatory state and microbial composition of the gut is related to nutritional status