5 research outputs found
ANALISIS KERAGAMAN GENETIK PADA IKAN KERAPU MACAN (Epinephelus fuscoguttatus) YANG TAHAN TERHADAP PENYAKIT YANG DISEBABKAN BAKTERI Vibrio alginolyticus
The research was purposed to analyse genetic similarity of tiger grouper (Epinephelus fuscoguttatus) inorder to select grouper that was resistant to Vibrio alginolyticus. In this study, fish were challenged with Vibrioalginolyticus and genetic similarity was examined by a PCR-RAPD method. Eight RAPD primers were used forPCR-RAPD analysis. The results showed that only three primers of RAPD primer (YNZ-22, UBC-456, danUBC-457) generated high number of RAPD fragments. The resistant group of tiger groupers generated higherpolymorph RAPD fragments than the susceptible groups. RAPD primer YNZ-22 and UBC-457 generated 82 %and 71 % of RAPD polymorph fragments from resistant group of fish and 71% and 60 % from susceptiblegroups, respectively. Primer YNZ-22 is a best genetic marker to analyse genetic similarity of tiger groupersproduced specific marker ranging from 1,2-2,0 kb. Genetic distances between the population of resistant fishand population of susceptible fish was 0.5091 and between individual of the resistant fish and susceptible fishwas 0.7032. Keywords: Genetik diversity, Epinephelus fuscoguttatus, V. alginolyticus, PCR, RAPD
Analisis Keragaman Genetik Pada Ikan Kerapu Macan (Epinephelus Fuscoguttatus) Yang Tahan Terhadap Penyakit Yang Disebabkan Bakteri Vibrio Alginolyticus
The research was purposed to analyse genetic similarity of tiger grouper (Epinephelus fuscoguttatus) inorder to select grouper that was resistant to Vibrio alginolyticus. In this study, fish were challenged with Vibrioalginolyticus and genetic similarity was examined by a PCR-RAPD method. Eight RAPD primers were used forPCR-RAPD analysis. The results showed that only three primers of RAPD primer (YNZ-22, UBC-456, danUBC-457) generated high number of RAPD fragments. The resistant group of tiger groupers generated higherpolymorph RAPD fragments than the susceptible groups. RAPD primer YNZ-22 and UBC-457 generated 82 %and 71 % of RAPD polymorph fragments from resistant group of fish and 71% and 60 % from susceptiblegroups, respectively. Primer YNZ-22 is a best genetic marker to analyse genetic similarity of tiger groupersproduced specific marker ranging from 1,2-2,0 kb. Genetic distances between the population of resistant fishand population of susceptible fish was 0.5091 and between individual of the resistant fish and susceptible fishwas 0.7032
Penggunaan Marka DNA dalam Seleksi Induk Ikan Kerapu Macan (Epinephelus fuscoguttatus) yang Tahan Terhadap Bakteri Vibrio alginolyticus
The objective of the present study was to use selected RAPD markers (Primer UBC-122 and YNZ-22) in parental selection to the bacterial Vibrio alginolyticus resistance in Tiger groupers (Epinephelus fuscoguttatus). 聽Eighteen fish parent samples were challenged with Vibrio alginolyticus and parental selection was examined by a PCR-RAPD method.聽 聽The results showed that from 18 fish samples, primer YNZ-22 generated 18 fragments while primer UBC-122 generated 8 fragments only (KHP, KB, HB, PB, MKH, MH, K and MK). 聽In addition, the average number of fragments from each samples generated from primer-YNZ was higher (3,2) than from UBC-122 (2,3). This indicates that primer YNZ-22 is better than primer UBC-122 to use in parental selection to the Vibrio alginolyticus resistance in Tiger groupers. 聽From the parental selection, five fish parents (KHP, PB, MH, K and聽 MK) were selected for their high resistance to the bacterial Vibrio alginolyticus
Prognostic model to predict postoperative acute kidney injury in patients undergoing major gastrointestinal surgery based on a national prospective observational cohort study.
Background: Acute illness, existing co-morbidities and surgical stress response can all contribute to postoperative acute kidney injury (AKI) in patients undergoing major gastrointestinal surgery. The aim of this study was prospectively to develop a pragmatic prognostic model to stratify patients according to risk of developing AKI after major gastrointestinal surgery. Methods: This prospective multicentre cohort study included consecutive adults undergoing elective or emergency gastrointestinal resection, liver resection or stoma reversal in 2-week blocks over a continuous 3-month period. The primary outcome was the rate of AKI within 7 days of surgery. Bootstrap stability was used to select clinically plausible risk factors into the model. Internal model validation was carried out by bootstrap validation. Results: A total of 4544 patients were included across 173 centres in the UK and Ireland. The overall rate of AKI was 14路2 per cent (646 of 4544) and the 30-day mortality rate was 1路8 per cent (84 of 4544). Stage 1 AKI was significantly associated with 30-day mortality (unadjusted odds ratio 7路61, 95 per cent c.i. 4路49 to 12路90; P < 0路001), with increasing odds of death with each AKI stage. Six variables were selected for inclusion in the prognostic model: age, sex, ASA grade, preoperative estimated glomerular filtration rate, planned open surgery and preoperative use of either an angiotensin-converting enzyme inhibitor or an angiotensin receptor blocker. Internal validation demonstrated good model discrimination (c-statistic 0路65). Discussion: Following major gastrointestinal surgery, AKI occurred in one in seven patients. This preoperative prognostic model identified patients at high risk of postoperative AKI. Validation in an independent data set is required to ensure generalizability