ESTUDO DA PARTIÇÃO DE ALBUMINA DE SORO BOVINO EM SISTEMAS AQUOSOS BIFÁSICOS

Neste trabalho, estudou-se a aplicação da extração líquido-líquido de albumina do soro bovino (BSA), por meio de sistemas aquosos bifásicos (SAB), compostos por polietilenoglicol (PEG), sulfato de sódio e água. Foram determinados dados de equilíbrio de fases para sistemas aquosos bifásicos PEG/Sulfato de sódio em temperatura ambiente.  Também foram avaliadas a influência dos fatores pH, temperatura, concentração de PEG e concentração de sal sob a partição da BSA, aplicando-se, inicialmente, um delineamento fatorial fracionado. Os resultados obtidos foram submetidos à análise de variância, sendo verificada a influência do pH, da temperatura e da concentração de Na2SO4 na partição da BSA. Para as variáveis significativas, foi empregado um delineamento composto central rotacional (DCCR). Os resultados obtidos no DCCR foram submetidos à análise de regressão e superfície de resposta, sendo determinadas as melhores condições para a otimização da partição da proteína. A extração da BSA pelo SAB utilizando PEG/Sulfato de sódio mostrou-se eficaz, utilizando-se de reagentes e etapas de processos bastante simples

<b>Optimization of protein extraction process from jackfruit seed flour by reverse micelle system

The extraction of protein from flour of jackfruit seeds by reverse micelles was evaluated. Reverse micelle system was composed of sodium dodecyl sulfate (SDS) as surfactant, butanol as solvent, and water. The effects of stirring time, temperature, molar ratio H2O SDS-1, concentration of butanol (mass percentage) and flour mass were tested in batch systems. Based on the adjusted linear regression model, only butanol concentration provided optimum extraction conditions (41.16%). Based on the analysis of surface response, the best extraction yield could be obtained at 25°C, stirring time of 120 min, mass of flour of 100 mg, and a ratio H2O SDS-1 of 50. Experimental results showed that a 79.00% extraction yield could be obtained

Optimization of protein extraction process from jackfruit seed flour by reverse micelle system

The extraction of protein from flour of jackfruit seeds by reverse micelles was evaluated. Reverse micelle system was composed of sodium dodecyl sulfate (SDS) as surfactant, butanol as solvent, and water. The effects of stirring time, temperature, molar ratio [H.sub.2]O [SDS.sup.-1], concentration of butanol (mass percentage) and flour mass were tested in batch systems. Based on the adjusted linear regression model, only butanol concentration provided optimum extraction conditions (41.16%). Based on the analysis of surface response, the best extraction yield could be obtained at 25[degrees]C, stirring time of 120 min, mass of flour of 100 mg, and a ratio [H.sup.2]O [SDS.sup.-1] of 50. xperimental results showed that a 79.00% extraction yield could be obtained.Nesse trabalho foi avaliado o processo de extracao de proteina da farinha de semente de jaca utilizando micelas reversas. O sistema de micelas reversas utilizado foi composto por dodecil sulfato de sodio (SDS) como surfactante, butanol como solvente, e agua. Foram testados os efeitos do tempo de mistura, temperatura, razao molar [H.sub.2]O DS.sup.-1], concentracao de butanol (porcentagem em massa) e massa de farinha. Baseado no odelo de regressao linear ajustado, apenas o fator concentracao de butanol apresentou uma condicao otima de extracao que foi de 41,16%. Baseado na analise de superficie de esposta, em 25[degrees]C, no tempo de mistura de 120 min, na massa de farinha de 100 mg e na razao [H.sub.2]O [SDS.sup.-1] de 50, e possivel obter o melhor rendimento de extracao. Os resultados experimentais mostraram que foi possivel alcancar rendimentos de extracao de 79,00%

Effect of the incorporation of lysozyme on the properties of jackfruit starch films

Jackfruit starch based biodegradable films containing lysozyme were characterized for their antimicrobial activity, thickness, solubility, water vapor permeability and mechanical properties. The biodegradable films had good appearance and antimicrobial activity against Micrococcus lysodeikticus. The thickness of the biodegradable films were not affected by the variation in pH, but the addition of lysozyme increased the thickness, the thickest films being those with the highest lysozyme concentrations. The variation in pH of the filmogenic solutions affected the solubility of the biodegradable films, water solubility being greatest at pH 7.0 and with the highest lysozyme concentration. The permeability of the biodegradable films was increased by incorporating lysozyme. The lysozyme concentration and pH variation caused changes in the mechanical properties. The addition of 8% lysozyme increased the tensile strength and Young’s modulus for all the pH values studied. With respect to the release of antimicrobial activity, the diffusion of lysozyme was shown to follow Fickian transport mechanism